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Showing 1 to 20 of 20 for “"Bacillus species"”.
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Recombinant Protein Production in Bacillus Species, Isolation and Methionine Production in Methionine-producing Microorganisms
… acnes<em> LAI were cloned into <em>Bacillus<em> species as the expression hosts. Unfortunately, the expressed <em>Bifodobacterium<em> LAI formed inclusion bodies and did not exhibit detectable enzymatic activities. Propeptide <em>Staphylococcus hyicus<em> lipase fused with <em>P. …
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Isolation and taxonomic analysis of Bacillus species in Anthrax negative cases at Central Veterinary Laboratory, Namibia
… emergence of anthrax-like infections caused by Bacillus spp. other than Bacillus anthracis is quickly becoming a topical issue. This brought more concern to the scientific community, guardians of public health, and veterinarians around the world. Some bacillus species that cause anthrax-like …
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Isolation, identification, and characterization of antibiotic-producing microorganisms from soil
… isolates 1 and 2 were identified as strains of Bacillus pumilus, whilst isolate 3 was found to be Bacillus subtilis (Chapter 3). This study further explored the chemical composition of the bacterial extract using ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass …
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Preserving spore dormancy
… germination during spore development. In Bacillus subtilis, L-alanine and L-valine create a metabolic conflict: they are required for biosynthesis during sporulation but also act as strong germinants. Wild-type populations resolve this by collectively depleting extracellular alanine and …
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Spore-forming bacteria as pharmaceutical factories
… of doping spores with extra DPA. Various Bacillus species, in the main B. subtilis and B. thuringiensis, have been engineered to produce, for the first time, fragments of a humanized monoclonal antibody: kindly provided by Medimmune. It is possible to produce antibodies inside the spore …
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Bacterial poly-gamma-Glutamic Acid (y-PGA) - a promising biosorbent of heavy metals
… investigated the production of HMW γ-PGA by five Bacillus species (B. licheniformis 1525, B. licheniformis NCTC 6816, B. licheniformis ATCC 9945a, B. licheniformis ATCC 9945a and B. subtilis (natto) ATCC 15245) in GS, C and E media for the removal of heavy metals in wastewaters. The highest γ-PGA …
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Isolation, identification and characterisation of novel actinobacteria from Zambian hot-springs
… and a potentially novel Verrucosispora species was identified. Screening the isolate identified a Streptomyces species which has hydantoinase, carbamoylase, amidase and nitrilase activities.The Streptomyces sp. hydantionase was cloned and functionally expressed in E.coli. The recombinant …
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Effect of Ultrasonication on Biofilm Forming Ability of Common Dairy Sporeformers
… is affected by these sporeformers such as Bacillus species, which are a significant cause of spoilage of dairy products. Thermal processes such as pasteurization have been used to inactivate pathogens, but some of the sporeformers and their endospores are resistant to such heat treatments. …
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Characterization of Bacillus Spore Membrane Proteomes and Investigation of Their Roles in the Spore Germination Process
… apparatus are well conserved in spore-forming species, such as the spoVA operon, each species may possess a different and possibly unique germinant recognition mechanism. The significance of several individual proteins in the germination process has been characterized. However, the mechanisms …
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The Roles of the Germination-Specific Lytic Enzymes CwlJ1, CwlJ2, and SleB in Bacillus anthracis Spores
The Bacillus anthracis spore is highly resistant to environmental stresses, but cannot cause anthrax until it successfully germinates. An essential step of germination, degradation of the cortex peptidoglycan layer, is carried out by germination-specific lytic enzymes (GSLEs). While the GSLEs of …
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Substrate Regulated Microaerophily and Chemotaxis by Pseudomonas jessenii strain VT10
… was also exhibited by five Pseudomonas and two Bacillus type strains from culture collection. Isolates identified with LSRMB formed a typical band of growth below the surface of low substrate (10 mg/l of peptone, tryptone, yeast extract and glucose) semi-solid medium. Surface growth was obtained …
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Arginase from Bacillus caldovelox
Nine Thermus strains, six thermophilic Bacillus species, six extremely thermophilic archaebacteria and other thermophilic bacteria were cultured in arginine-supplemented media and screened for arginase, arginine deiminase and arginine decarboxylase activity by a reversed-phase liquid chromatography …
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Sustainable management of tannery hair waste through composting
… crude enzyme, expressed by the bacteria of Bacillus species, improved the biodegradation of hair during the controlled composting process. The compost stability evaluated by monitoring the humification parameters and carbon to nitrogen ratio, indicated that the final product reached the …
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In planta studies of the corn pathogen Pantoea stewartii subsp. stewartii and applications of a corn-based industrial byproduct
… that the highest growth rate and yield was from Bacillus species, some of which may have probiotic benefits to fish. Ultimately, the collective outcomes from these projects in basic research about a bacterial corn pathogen and applied research about beneficial microbes grown on a corn-based …
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The role of γ-polyglutamic acid in a circular economy: cost-effective production and wide-ranging applications of a bio-derived, biodegradable, and nonimmunogenic biomaterial
… microorganisms, especially members of the Bacillus species. γ-PGA is synthesised through several enzymes and complexes which racemise and polymerise L- glutamic acid or D- glutamic acid. This non-toxic microbial product can have variable viscosity, is water soluble, biodegradable, and …
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The Infection Control Risks Associated with the Use of Mobile Technology in Healthcare
… Rothia terrae, Corynebacterium freneyi and Bacillus although the majority were Staphylococci, many exhibiting antibiotic resistance. The culture independent analysis revealed a far greater species diversity than initially indicated, with Bacillus species being particularly abundant. The …
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Selection of bacterial species from wastewater for potential production of poly (γ-glutamic acid): isolation, characterisation and growth kinetics
… commonly, γ-PGA production has been studied in Bacillus species, such as B. subtilis and B. licheniformis. Bacillus species are ubiquitous in the environment, including an association with domestic wastewater treatment. Its enrichment has been associated with improved treatment processes. The …
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Effect of Arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria on glomalin production
… to examine the effect of fungal and host plant species on glomalin production by comparing the amounts of glomalin produced by Glomus clarum, G. intraradices, and G. mosseae in association with corn (Zea mays L.), in addition to examining differences in the ability of corn, pea, and wheat …
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Construction and analysis of novel controllable expression vectors for Bacillus subtilis
Summary The gram-positive bacterium Bacillus subtilis is well-known for its contributions to agricultural, medical, and food biotechnology and for the production of recombinant proteins. At present, about 60% of the commercially available technical enzymes are produced by Bacillus species. …