Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 28 for “"BV2"”.
-
Role of ICAM-1, a cell adhesion molecule, in morphological changes of BV2 microglial cell
… for the microglial activation using immortalized BV2 microglial cell line. After seeding in the plastic, BV2 cells showed their morphological changes, from the round shape to bipolar or multipolar (spread) shape, according to time course. The treatment of LPS (1 ㎍/ml) influenced their …
-
LPS, A TLR-4 AGONIST AND VIPER A TLR-4 INHIBITOR UPREGULATE PHAGOCYTOSIS OF ZYMOSAN IN BV2 CELLS
… cerevisiae</em><em> </em>in microglia like BV2 cells. Furthermore, we examined the possible involvement of a β-glucan receptor on zymosan phagocytosis using laminarin, a soluble β-glucan molecule. In addition, we investigated the potential impact of TLR-4' The LPS receptor' on zymosan …
-
Obesity and neuroinflammation : the impact of adipocyte-derived extracellular vesicles on microglial cell signalling
… techniques, two microglial cell lines, HMC3 and BV2 cells were characterised under normal and inflammatory conditions (1μg/mL and 10μg/mL of LPS). AT-EVs were isolated and characterised using ultracentrifugation and light scattering techniques (dynamic light scattering and nanoparticle tracking …
-
Characterization of cathepsin B cysteine protease from Naegleria fowleri excretory-secretory proteins exerting immunomodulatory effects
… responses induced by Nf-ESPs were analyzed in BV2 microglial cells. Several cytokines, particularly IL-1α and TNF- α were highly up-regulated by Nf-ESPs. Also, activation of P38 and JNK was observed in BV2 cells treated with Nf-ESPs. The results collectively suggest that Nf-ESPs may play an …
-
Continuity Properties and Variational Problems Involving the Determinant of the Hessian
… a continuous function from the intersection of BV2 and W(1, infty)(D), with a suitable topology, into the space of distributions. Here BV2 is the space of functions whose first derivatives are functions of bounded variation. This function space is naturally chosen to investigate a variational …
-
Exploring the bidirectional interface between stress and innate immunity: a focus on glucocorticoid and TLR4-MyD88 signalling
… in immunocompetent cells. In these 2 studies, BV2 microglia-like cells, RAW264.7 macrophage-like cells and adult primary microglia were utilised. Study 2 demonstrated a biphasic innate immune response to glucocorticoids, where low concentrations of corticosterone pre-exposure primed, while a …
-
Acetate Increases Brain Purine Metabolism And Glial Cell Culture Fatty Acid Content: Studies Investigating Glyceryl Triacetate's Therapeutic Mechanism Of Action
… metabolism and inflammation, we used in vitro BV2 microglia and primary astrocyte cell cultures. These experiments showed that in vitro acetate treatment had distinct cell-type specific effects on LPS-induced changes in adenosine metabolizing enzyme and receptor levels. These data suggest that …
-
Investigation of insulinotropic and cytoprotective effects of selected amphibian skin peptides
… cells, BRIN-BD11, and microglial cells, BV2. The purity of commercially synthesised selected peptides was confirmed using reversed-phase HPLC. Insulin-releasing effects of graded concentrations of selected peptide (0 - 3μM) were studied in a 20 min acute test using BRIN-BD11 cells. For …
-
Effects of β-funaltrexamine on lipopolysaccharide-induced inflammation and behavior
… behavioral deficits using a preclinical model. BV2 murine microglial cells were co-incubated with β-FNA and LPS at various times, while osmotic drug pumps were surgically inserted into mice that continually dispensed β-FNA for six days prior to LPS injection. Depressive-, anxiety-, and …
-
The role of cytosolic phospholipase A2 in microglial signaling pathways during neuroinflammation
… inhibition and siRNA knockdown of cPLA2 in BV2 microglial cells. Interestingly, unlike macrophages, inhibition of COX1[slash]2 did not result in significant decrease in oxidative[slash]nitrosative responses in BV-2 cells. Instead, lipoxygenase (LOX) inhibition, or more specifically LOX-12 …
-
role of pentazocine in an in vitro model of hypoxia/reoxygenation
… following hypoxia/reoxygenation condition. BV2 cells were exposed to 3 hours of hypoxia and 24h of reoxygenation. Cells were treated with sigma-1 agonist (+)-Pentazocine, various bifunctional sigma-1 agonists conjugated with lipoil function and with lipoic acid alone. We assessed cell …
-
Progress Towards Understanding Of Mechanisms Of Action Of Potent Multifunctional Disease Modifying Therapeutics For Parkinson's Disease & Investigating The Methamphetamine-Induced Striatal Microglia Activation.
… expression profile of culture microglia cells (BV2 cells). We determined to evaluate the effect of different concentrations of methamphetamine and lipopolysaccharide (gram negative endotoxin which causes strong immune response) on cultured microglia cells alone and cultured microglia cells which …
-
Phosphatidylinositol 4-phosphate 5-kinase Involved in Neuroglial Toll-like Receptor Signaling
… PIP5K-alpha knockdown stable cell lines of BV2 microglia were developed using lentiviral short hairpin RNA (ShRNA) expression system. PIP5K-alpha ShRNA significantly reduced PIP5K-alpha protein and mRNA levels. PIP5K-alpha knockdown significantly suppressed LPS-induced production of …
-
The Role of Neuroinflammation in Alzheimer's Disease
… of a neuroinflammation assay that uses a BV2 microglial cell line to test the inflammatory potential of synthetic amyloid beta 42 aggregates. In conjunction with a liposome-permeability assay and an immunoprecipitation assay, this assay has been used to validate a proprietary amyloid beta …
-
Microglial Modulation of Neuronal Autophagy: Uncovering Cytokine-Mediated Pathways
… experiments revealed that conditioned media from BV2 microglia downregulated autophagy in SH-SY5Y neuroblastoma cell line. Two strategies to screen the microglial secretome for autophagy-modulating molecules were employed: direct analysis of secreted cytokines and functional screening via knockout …
-
Bolaamphiphiles as a novel drug delivery system in the treatment of diseases of the brain
… CNS-representative and other cell lines - BV2 microglia, SH-SY5Y neurones, LN229 glioblastoma, HEK-293T epithelia, hCMEC/D3 endothelia and HASTR/ci35 astrocytes. In vivo studies were performed using C57BL/6 male mice. A novel methodology was developed to permit synthesis of the novel BAs …
-
The Novel Long Non-Coding RNA Nostrill Regulates Intestinal Epithelial Defense Against Cryptosporidium parvum and Modulates Microglial-Mediated Neurotoxicity
… Nostrill was induced by LPS stimulation in BV2 cells, primary murine microglia, and in cortical tissue of LPS-injected mice. Induction of Nostrill is NF-κB-dependent and promotes inducible nitric oxide synthase (iNOS) expression and downstream nitric oxide (NO) production. In vitro studies …
-
Investigation of the ABI3 protein in microglia and its association with Alzheimer’s disease in TgCRND8 mouse model
… migration was seen after knocking down ABI3 in BV2 cells and similar results were observed in ABI3 KO primary microglia. The consequences of ABI3 KO could also be seen in several aspects of amyloid-beta plaques properties since the number of the plaques as well as average plaque size were …
-
Development of Novel, Brain Permeable, mPGES-1 Inhibitors for Inflammatory Conditions
… production in both human (SK-N-AS) and murine (BV2) cells better than their parent compound (C3). To follow up on our initial screen IC¬¬50 values of UT-11 and compound 19 were calculated. We obtained 0.10 and 2.00 μM for UT-11 and 0.43 and 1.55 μM for compound 19 against human and murine cells, …
-
The G-protein coupled receptor CMKLR1/ChemR23: Studies on gene regulation, receptor ligand activation, and HIV/SIV co-receptor function
… important for transcription of cmklr1b in mouse BV2 cells. Cmklr1a shows expression in organs such as heart and lung whereas cmklr1b is suggested to be an inducible transcript up-regulated by stimulation with all-trans retinoic acid (ATRA). The interaction between CMKLR1/ChemR23 and TIG2/chemerin …
Page 1 of 2