Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"BMDMs"”.
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The role of macrophage intracellular lipid partitioning in glucose and lipid homeostasis during obesity
… rate in bone marrow-derived macrophages (BMDMs) and ATMs, and that loss of rate-limiting enzyme in de novo PC synthesis pathway, CTP:phosphocholine cytidylyltransferase a (CCTa) diminished saturated FFA-induced inflammation in BMDMs. In the second part, I show that macrophage-specific CCTa …
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Phagosome proteomes in activated macrophages
… of all, a comparison of phagosome proteomes of BMDMs and RAW 264.7 cells was performed, suggesting that there are significant differences for a large number of proteins including important receptors such as mannose receptor 1 and Siglec-1. Moreover, BMDM phagosomes mature considerably faster …
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Elucidating the mechanism of mitochondrial superoxide production in pro-inflammatory macrophages
… I also used bone marrow derived macrophages (BMDMs) expressing the alternative oxidase (AOX) to determine the influence of an oxidised CoQ pool on mitochondrial superoxide production. Using this approach, I demonstrated that LPS induced mitochondrial superoxide production is driven by an …
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Exploring Parkinson’s disease associated LRRK2 and PINK1/Parkin signal transduction pathways within immune cells
… and mouse bone marrow derived macrophages (BMDMs). All infection experiments were conducted using P. aeruginosa, a Gram-negative opportunistic bacterium. In addition, I aimed to explore LRRK2 activation within neutrophils isolated from the sputum samples of cystic fibrosis (CF) patients, who …
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Transcriptome-Wide Characterization of APOBEC1-Catalyzed RNA Editing Events in Macrophages
… activity in bone marrow derived macrophages (BMDMs). Using a comparative RNA-Seq screen, I identified more than 100 novel APOBEC1 editing events in BMDMs. This APOBEC1 activity occurred in two distinct editing patterns and fell within evolutionarily conserved regions of transcript 3'UTRs. …
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Battling Bacteria: Characterizing the NOD-Like Receptor (NLR) Immune Response to <i>Brucella abortus</i> and <i>Borrelia burgdorferi</i> in Host-pathogen Interactions
… models and bone marrow-derived macrophages (BMDMs) challenged with Brucella abortus, we found that canonical inflammasomes in murine models were protective against brucellosis through the initiation of inflammatory cell death called pyroptosis. In contrast, the inhibition of inflammation by …
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Transcriptional Regulation of NLRC4 Inflammasome by IRF8
… activation in bone marrow-derived macrophages (BMDMs) is greatly dependent on interferon regulatory factor 8 (IRF8). NLRC4-mediated caspase-1 activation and subsequent production of the inflammasome-dependent cytokines IL-1β and IL-18 and cell death were impaired in IRF8-deficient cells. IRF8 …
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HP Turns 17: T helper 17 cell response during hypersensitivity pneumonitis (HP) and factors controlling it
… phagocytosis ability of both WT and TLR2/9 DKO BMDMs (Bone Marrow Derived Macrophages) or BMDCs (Bone Marrow Derived Dendritic Cells). In vivo, there was no significant difference in the phagocytosis ability of both WT and TLR2/9 DKO alveolar macrophages. Although we found that both TLRs 2 and 9 …
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Mechanistic Insights into the Effects of Exercise on Inflammatory Responses
… programs were performed by the exercise group. BMDMs were isolated and were then stimulated with several inflammatory inducers. Our data from qPCR and Western Blotting demonstrate that NF-B mediated genes have been downregulated following moderate exercise and up-regulated following high …
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The Hypoxic Adenosine Response Modulates Macrophage Differentiation and Contributes to Lung Disease
… fibrosis in bone marrow derived macrophages (BMDMs) and in vivo models of bleomycin-induced pulmonary fibrosis. These players will be valuable as potential clinical targets to halt differentiation of macrophages into the reparative AAM subtype and attenuate their subsequent pro-fibrotic role. …
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The role of PLK1 in NLRP3 inflammasome activation
… are mutually exclusive events in murine BMDMs (Bone Marrow Derived Macrophages) and that PLK1 is an important regulator of NLRP3 inflammasome activation. Using two different potent and specific PLK1 inhibitors, I demonstrated that PLK1 inhibition impairs the NLRP3 inflammasome pathway. …
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Inflammasome signalling during Salmonella Typhimurium infection
… bacteria counts and IL-1β production to that of BMDMs derived from C57BL/6 mice lacking proteins involved with, or suspected to be involved with, innate immune activity. Amongst the proteins I studied, caspase recruitment domain 9 (CARD9) inhibited inflammasome-mediated IL-1β production. Multiple …
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Assessing The Outcomes of Blocking Ccl2-Ccr2 Signaling Axis On Breast Cancer Brain Metastasis
… of bone marrow-derived myeloid cells (BMDMs), from peripheral blood into the brain tumor microenvironment (TME), eventually promoting brain metastasis outgrowth by driving immune suppression. Here, in this project we have been aiming to develop effective therapies by immune-modulating …
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Innate sensing and regulation during enteric caliciviruses infections
… response to infection with MNV1 in primary BMDMs and RAW264.7 cells, with a significant increase in viral titres following infection in RIG-I- and STING-deficient cells. We also show that STING is non-canonically activated in MNV1-infected cells partly in a RIG-I dependent manner. …
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Cluster Determinant 44 (CD44) Receptor and Gout: Mechanistic Role in Pathogenesis and Therapeutic Targets
… assess the role of CD44 using in vitro models of BMDMs of different genotypes and used Extra cellular domain shedding to study the impact on MSU phagocytosis, NF-kB translocation, NLRP3 inflammasome activation, and proinflammatory cytokine production in human macrophages. We also aimed to study …
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Nterleukin-4 responsive dendritic, macrophage/neutrophil cells are dispensable for host resistance against Leishmania Mexicana infection in mice
… IL‐12p70 and IL‐10 produced by BMDCs and BMDMs were similar. Nevertheless, nitrite/urea production was not affected. Together, this study suggests that, unlike L. major, IL-4Rα signaling on DCs and macrophage/ neutrophil cells does not contribute to the susceptibility or resistance to …
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Epigenetic regulation of gene expression by BRD4 in cancer and innate immune response
… Brd4-deficient bone marrow-derived macrophages (BMDMs) displayed blunted NLRC4 inflammasome activation with decreased caspase-1 activation, Asc oligomerization, IL-1 maturation, gasdermin-D (Gsdmd) cleavage, and pyroptosis upon bacterial infection. RNA-seq results unveiled that the ablation of …
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The role and host-directed targeting of long non-coding RNAs in macrophage polarization during Mycobacterium tuberculosis infection
… IFN-γ and IL-4/IL-13 pre-stimulated BMDMs infected with Mtb. Comparatively, gapmeR knockdown of lncRNA-612 reduced Mtb growth and increased pro-inflammatory cytokine production in IFN-γ pre-stimulated mouse and human macrophages. In mouse macrophages, these responses were mediated by …
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Mechanisms of Innate Immune Dysfunction During Mycobacterium tuberculosis and HIV Co-infection
… evidenced by greater Mtb proliferation in BMDMs and the lung of MGL-1 deficient mice. Human CLR expression was further evaluated in a humanized mouse model of Mtb, HIV, and co-infection. Surface expression of MGL was increased following early HIV infection (two weeks) but was later …
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An Investigation into the Function of CDS2 in Phosphoinositide Synthesis in Murine Macrophages
… In CDS2-KO bone marrow derived macrophages (BMDMs), basal PIPn levels are maintained, albeit with a clear steady-state accumulation of certain molecular species of PA, DAG and triacylglycerol (TAG). To interrogate the changes in pathway activity that underlie these effects, mass spectrometry …
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