Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"BMAL1"”.
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Non-Photic Mechanisms of Entrainment In Bmal1 Deficient Conditions
… clock protein brain and muscle ARNT-like factor (BMAL1), which is a transcription factor critical for maintaining cellular rhythmicity. Loss of the protein in the PVN of the hypothalamus can lead to arrhythmicity, but can be rescued by exercise. In the case of acute myeloid leukemia, this protein …
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The role of circadian rhythms in reproduction: development and fertility in the bmal1 null mouse.
… including reduction of fertility. The bmal1 mouse provides an animal model for central and peripheral loss of rhythmicity. Herein the reproductive function and postnatal development in the bmal1 knockout mouse has been evaluated. The reproductive capability of the heterozygous breeding …
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The Role of the Circadian Clock in the Paraventricular Nucleus of the Hypothalamus in Diurnal Energy Balance
… loss of the circadian transcription factor, BMAL1, in the paraventricular nucleus (PVN) of the hypothalamus has revealed its importance in driving rhythms in energy intake and metabolism, its transcriptional functions across time and in individual cell types in the PVN are not well …
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Circadian Secretion of Glucagon-like Peptide-1
… parallels the expression of the core clock gene, Bmal1. However, the mechanisms by which the GLP-1 secretory rhythm is established and its role in maintaining diurnal glycemic homeostasis under normal and obesogenic conditions remain largely elusive. Thus, I hypothesized that western diet …
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Architecture of the Mammalian Circadian Repressive Complex
… loop composed of positive regulators (CLOCK/BMAL1) and repressors (CRY1/2 and PER1/2). To understand what drives periodicity within this clock, I took structural approaches with the hope of identifying atomic-level details that inform behavioral outputs. Despite high sequence identity, null …
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Screening for kinases regulating subcellular localization of clock proteins using human kinome library
… 효소 스크리닝 20 C. VRK3 27 1. hVRK3는 mCRY1/2, mPER2, BMAL1 단백질의 세포 내 위치를 조절한다 27 2. hVRK1과 hVRK2는 mCRY1의 세포 내 위치를 바꾸지 않는다 30 3. hVRK3는 mSTAT3의 세포 내 위치에는 영향을 미치지 않는다 32 4. hVRK3는 핵 안에서 작용하여 생체시계 단백질 -mCRY1, mPER2, BMAL1- 을 세포질에 머물게 한다 34 D. PRKRA 38 1. hPRKRA는 mPER2 특이적으로 세포 내 위치를 변화시키고, mCRY1과 mPER2, …
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REGULATION OF CIRCADIAN CLOCKS AND METABOLISM BY ARYL HYDROCARBON RECEPTOR
… brain and muscle AhR nuclear translocator like-1(BMAL1), disrupting circadian locomotor output cycle kaput (CLOCK)/BMAL1 complex activity, and leading to the suppression of period1 gene (Per1) expression rhythm. My studies indicate that AhR activation by its agonists …
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Circadian Timekeeping of Body Temperature by the Mammalian Suprachiasmatic Nucleus
… recordings. We show that genetic deletion of Bmal1, a non-redundant clock gene for driving the cell-autonomous circadian clock, broadly in the forebrain inclusive of the SCN sufficiently abolished the circadian rhythmicity of Tb. In contrast, genetic deletion of Bmal1 specifically from the …
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Modulation of the Hippocampal Neurogenic Program by the Circadian Clock Machinery and the small GTPase, Rasd1/ Dexras1
… feedback loop (TTFL), which includes Bmal1 and Period2. In this thesis, I demonstrate the presence of the molecular clock and of rhythmic cell proliferation in the SGZ. The absence of Period2 abolishes the gating of cell cycle entrance of QNPs, whereas the genetic ablation of Bmal1 …
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EVALUATING THE CHEMOTHERAPEUTIC EFFECTS OF SR9009 AND ESOMEPRAZOLE ON ORAL SQUAMOUS CELL CARCINOMA USING CIRCADIAN-TAILORED INNOVATIVE THERAPEUTIC APPROACHES
… we analysed the expression of clock genes, BMAL1 and REV-ERBα by Immunohistochemistry, Immunofluorescence, and RT-qPCR in the tumor samples of these cell lines inoculated in mice to generate OSCC in vivo. Chemotherapeutic effects of SR9009 and Esomeprazole were determined in vitro using …
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REGULATION OF CIRCADIAN CLOCKS AND METABOLISM BY SYNTHETIC AHR AGONIST BETA-NAPHTHOFLAVONE IN MICE
… components: Brain and Muscle Arnt-Like protein1 (BMAL1), Circadian Locomoter Output Cycles Kaput (CLOCK), Period (PER), Cryptochrome (CRY), Nuclear Receptor family1 D1 (REV-ERB) and Retinoic acid-related Orphan Receptor (ROR). Circadian clock disruptions, through environmental changes to …
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Using Translational Switching to Investigate the SCN Clockwork
… I developed novel translationally switchable Bmal1 (tsBMAL1) and Period2 (tsPER2), as well as using a translationally switchable copy of CRY1 (tsCRY1) developed previously in the lab, to investigate the SCN clockwork. First, I show how generation of tsBMAL1 provided conditional and reversible …
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Epigenomic and Transcriptional Regulation of Hepatic Metabolism by REV-ERB and Hdac3
… competes with RORα for genomic binding at Bmal1 and Npas2 genes, and drives a diurnal binding of RORα. We then identified thousands of competing sites by RORα ChIP-seq, many of which are in close proximity of clock and metabolic genes. We also discovered many RORα binding sites with no …
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Analysis of Circadian Rhythm Using a Novel SCN-Specific Cre Transgenic Mouse Line
… at the SCN. Next, I generated NMS-Cre mediated Bmal1 conditional knockout animals to study behavioral rhythm output when most of the SCN neurons are without a molecular oscillator. The NMS-Cre(+);Bmal1flox/flox animals have essentially normal circadian rhythm of locomotor activity. Then, I …
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Investigation of the Conservation of Biological Function in Transposable Elements using a Multiple Alignment Strategy
… align with structural expectations. For example, BMAL1/CLOCK-bound motifs, which contain helix-loop-helix (bHLH) domain, show accelerated patterns at the central region, which is known to be structurally flexible, flanked by more conserved positions. This pattern supports the idea that certain …
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Mathematical Modeling of Circadian Gene Expression in Mammalian Cells
… between the repressor PER and the activator BMAL1 is relaxed for robust oscillations. By studying the modified extended models, we found that the auxiliary positive feedback loop, rather than the auxiliary negative feedback loop, makes the oscillations more robust, yet they are similar when …
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Actin's role in signaling light-induced phase shifts in the mammalian circadian clock
… in mice rendered genetically arrhythmic, the BMAL1 -/- mice. Secondly, I measured actin state after light exposure, and found that brief light pulses induced a breakdown of polymerized actin. By injecting the actin-stabilizing chemical jasplakinolide directly into the mouse SCN, I demonstrated …
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Multi-omic Studies of Neutrophils Reveal Programmed Deactivation Phenotypes After Inflammatory Stimulus
… the upstream regulator of neutrophil aging, BMAL1, was consistently expressed among the top transcripts at all time points. Functional assays indicated early phagocytic activity; however, whilst ingestion and phagosome acidification continued out to at least three hours, this occurred at a …
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Development of fish embryonic stem cell lines as tools for basic and applied studies of interest in aquaculture
… main components of the molecular clock (clock, bmal1, cryptochrome1a, period1, period2 and period3) under controlled light-dark cycles and constant conditions (constant light, constant dark), as well as the response to nocturnal light pulses in this cell line. This study has allowed us to …