Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"BHMT"”.
-
The Effect of High Salt Intake on Betaine-Homocysteine S-Methyltransferase Expression in the Guinea Pig
BHMT is the only enzyme known to metabolize betaine, leading to the hypothesis that BHMT is down-regulated under conditions requiring the accumulation of betaine, such as high salt intake. BHMT is expressed in the livers of mammals and only a few species such as pig, guinea pig, and primates …
-
Developmental and evolutionary analysis of betaine homocysteine methyltransferase genes
… Betaine-homocysteine methyltransferase (BHMT) and BHMT-2 methylate homocysteine to form methionine using betaine and S-methylmethionine, respectively. Betaine is produced de novo from choline and is also found in the diet, whereas S-methylmethionine is only made in plants and fungi and so …
-
Kinetic characterization of methyl donor substrates and inhibitors of human, pig, and rat liver betaine homocysteine S-methyltransferase (BHMT)
This Thesis was approved for publication on 2015-05-01 at 08:42.
-
Betaine-Homocysteine Methyltransferase: Tissue Distribution, Nutrient Regulation, Genomic Organization, and Promoter Isolation
… and the nucleotide sequence of the human kidney BHMT has been shown to be identical to liver BHMT. Initial rate data was plotted according to the method of Hanes and the Michaelis Constants were estimated to be 2160 muM and <10 muM for betaine and L-Homocysteine, respectively. These constants …
-
Role of Betaine-Homocysteine S-Methyltransferase in Homocysteine Methylation
… but 5 mumol/L D,L-CBHcy completely inhibited BHMT activity indicating, that D,L-CBHcy was a specific inhibitor of BHMT. To address whether BHMT inhibition caused hyperhomocysteinemia, mice were injected with 1mg of D,L-CBHcy. This reduced BHMT activity (87%) and elevated total plasma Hcy …
-
The Role of Potassium in Betaine-Homocysteine Methyltransferase
… and betaine-homocysteine methyltransferase (BHMT). Methionine synthase function depends on adequate availability of vitamin B12 and folate which links cardiovascular disease risk to nutritional status. The Hcy remethylation pathway through BHMT, however, requires only the substrate betaine …
-
New Insights Into the Structure and Function of Betaine-Homocysteine S-Methyltransferase
… Betaine-homocysteine S-methyltransferase (BHMT) is one of two enzymes capable of remethylating Hcy to methionine. We report that BHMT is susceptible to conformation-dependent oxidative inactivation. Two oxidants, methyl methanethiosulfonate (MMTS) and hydrogen peroxide (H2O2), cause a loss …
-
Interrelationships Among Sulfur Amino Acids, Choline and Betaine in the Chicken: Effects on Betaine-Homocysteine Methyltransferase Activity, Homocysteine Utilization and Choline Bioavailability
… hepatic betaine-homocysteine methyltransferase (BHMT) activity. In Assay 1, use of a methionine-deficient purified diet caused a 3-fold increase $(P<0.05)$ in BHMT activity, and addition of choline or betaine further increased $(P<0.05)$ activity. In Assay 2, use of a methionine-deficient …
-
Recombinant Human Betaine -Homocysteine S -Methyltransferase: Discovery as a Zinc Metalloenzyme, and Regulation of Activity by Redox Status
… amount of 5Cys/Ala in solution. Finally, when BHMT-5Cys/Ala is in the presence or absence of reducing agent, and in the former condition the reducing agent is removed by gel filtration, the number of DTNB-modifiable residues is three and one, respectively. These data indicate that the redox …
-
Maternal rumen-protected methionine supplementation alters dairy calf development, postnatal growth, plasma biomarkers, and liver one-carbon metabolism
… of betaine-homocysteine S-methyltransferase (BHMT), methionine synthase (MTR), and cystathionine-beta-synthase (CBS) in liver tissue. The activity of BHMT and CBS increased in MET calves between d 4 and 14, with a peak at 28 d. Despite a linear increase from d 4 to 28, the activity of MTR in …
-
Physiological and molecular mechanisms associated with performance, immunometabolic status, and liver function in transition dairy cows fed rumen protected methionine or choline
… response to CHOL supplementation, expression of (BHMT) and MTR and BHMT enzyme activity did not change (P > 0.05). Furthermore, overall MTR enzyme activity was lower (23.5 vs. 29.6 nmol product h-1 mg protein-1; SEM = 2.9; P = 0.05) in CHOL cows. In terms of AA profile, MET supplementation led to …