Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"BHK"”.
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Rationale Ansätze zur Prozessentwicklung eines Anti-EGF-IL2-Fusionsproteins mittels einer rekombinanten BHK-Zelllinie
… a recombinant baby hamster kidney cell line (BHK). The thesis is integrated within a cooperation project with MERCK KGaA, Darmstadt. The classical process development part includes the investigation of process parameters regarding cell growth and product formation. The second part describes …
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Three Essays in Intuitionistic Epistemology
… intuitionistic logic which reflects the intended BHK semantics of intuitionism. The fundamental assumption concerning intuitionistic knowledge and belief is that it is the product of verification. The BHK interpretation of intuitionistic logic has a precise formulation in the Logic of Proofs and …
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On Berglund-Hübsch-Krawitz Mirror Symmetry
… orbifolds that have Berglund-Hübsch-Krawitz (BHK) mirrors. These Calabi-Yau orbifolds are certain finite symplectic quotients of hypersurfaces in weighted-projective space. First, we will describe their birational geometry using Shioda maps and prove that so-called alternate mirrors are …
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Characterization of the 37-kDa/67-kDa laminin receptor as the cell surface receptor for the cellular prion protein
… these cells. In addition, baby hamster kidney (BHK) cells transfected with recombinant Semlik-Forest virus RNAs overexpressed human PrP and human LRP at their cell surface, the latter one orientated as a type II transmembrane protein with its C-terminus outside and its N-terminus inside the …
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Synthesis and Microscopic Characterizations of Virus Nanoparticle-Based Composite Materials
… in vitro cell studies of baby hamster kidney (BHK) cells. As a result, in contrast to cells grown on PVA or PVA-TMV substrate, BHK cells show better adhesion and spreading on the RGD mutant TMV-PVA fibrous mats. Similarly, the breast cancer stem cell M605 also displayed better spreading on …
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REGULATION OF GLYCOSYLATION IN MAMMALIAN CELL LINES: INSIGHTS FROM STRUCTURAL GLYCOMICS, ANALYSIS OF GLYCOSYLATION-RELATED GENES AND FUNCTIONAL RNAI SCREENS
… used mammalian cell lines, namely CHO-K1, BHK-21, HEK293, COS-7 and 3T3, were characterized by a combination of mass spectrometric and chromatographic techniques. Such glycomic profiling revealed several immunogenic non-human N-glycan structures in BHK-21 and 3T3 cells. The cell lines were …
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Studies of structure-function relationships in two human coagulation proteins: factor XII and prothrombin
… Four human FXIIcDNA constructs were expressed in BHK cells, using the pNUT vector: FXII wild type,FXIIA1-20, FXIIA5-20 and FXIIA28-69. The FXII wild type and FXIIA28-69 were secreted into the media at -5-10 [tg/mL while FXII01-20 and 5-20 were expressed in the cells but not secreted into the …
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Functional Analysis of Wild-Type LGN and T450 Mutants
… cancer. LGN was transiently expressed in BHK-570 tissue culture cells using a pCMV-LGN plasmid and LipoD 293 reagent. Protein expression of wild-type LGN was confirmed by both Western blot and immunocytochemistry of fixed, permeabilized cells using anti-GPSM2 antibody. Furthermore, the …
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Infection and Dissemination of TaV-GFP Tagged Sindbis in Aedine Mosquitoes and Cell Lines
… reveal virus transit. Virus stocks were grown in BHK-21 vertebrate cells and C7-10 mosquito cells. Two Aedes albopictus mosquito cell lines, C7-10 and C6/36, were then challenged with vertebrate and mosquito grown reporter virus. Evidence of GFP were seen as early as 6 hours post infection (p.i.) …
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Aspects of the collocational analysis of meaning with special reference to some Biblical Hebrew anatomical idioms
… expression being discussed are capitalized. BHK/S is used as the source of quotations from the Hebrew Bible, although its division of cola is not displayed; the caesura (athnach) is sometimes indicated by the use of a new line, or, if only one line of text is displayed, by a double space …
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Η μεταβολομική ως υψηλής απόδοσης βιομοριακή ανάλυση στη μηχανική κυτταροκαλλιεργειών
… στη μεταβολική φυσιολογία κυτταροκαλλιεργειών BHK (baby hamster kidney - νεφρικά κύτταρα νεογνού χοιριδίου) που ήδη χρησιμοποιούνται στη φαρμακοβιομηχανία για την παραγωγή θεραπευτικών πρωτεϊνών. Έγινε χρήση GC-MS μεταβολομικής ανάλυσης σε δείγματα που συλλέχθηκαν από υψηλής κυτταρική …
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Multi-species genome-wide CRISPR screens identify conserved suppressors of cold-induced cell death
… both a cold-sensitive (K562) and cold-resistant (BHK-21) cell line, and uncover GPX4 and related selenocysteine incorporation genes as important for protection against cold-induced cell death. Using genetic knockdowns, along with overexpression of GPX4, we confirm our findings and demonstrate that …
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Purification of glycoproteins from herpes simplex virus
… determinants. HSV-1 and HSV-2 were produced in BHK-21 cells and labelled with either (³H)-glucosamine ((³H)-gln) or a mixture of (¹⁴C)-amino acids ((¹⁴C)-aa). Analysis of the radiolabelled products by analytical sodium dodecyl sulphate polyacrylamide gel electrophoresis (SOS-PAGE) and …
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High density animal cell culture systems using porous supports
… Vero cells and transformed baby hamster kidney (BHK) cells which produced human transferrin were grown in three different reactor configurations: a fixed bed ceramic perfusion system, an airlift systemwith draft tube made of porous ceramic and a stirred tank configuration which used porous …
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Evaluation of arthropod-borne virus recombination potential and phenotype: In vitro and in vivo analysis of flavivirus and alphavirus model systems
… YFV replicon co-infection levels of ~2.4x106 in BHK-21 (vertebrate) cells and ~1.05x105 in C710 (arthropod) cells. Additionally, to address concerns that a flavivirus vaccine/wild-type recombinant virus might have a high mosquito infectivity and/or vertebrate virulent phenotype, the YFV backbone …
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The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation by HSP-90
… protein expression levels above that observed in BHK cells transfected with wild-type (WT)-CFTR and CFTRhis10. The Flag-CFTR expression was not only confirmed via immunofluorescent detection, but was also shown to be functional using a standard iodide efflux assay. The HEK-293 Flag-CFTR cells …
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Functional genomic study and bioinformatic analysis on natural bioactive peptides
… 5, the sequence modification was employed in the BHK-bombinin by replacing the glutamic acid with lysine at the 23rdposition from amino terminus, which increased the net charge and expanded thenonpolar face of the original peptide. According to the results from in vitro function alanalysis, this …
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Metabolische Veränderungen und Zelltod in neuralen Zellen durch "Advanced Glycation Endproducts"
… oder verhindern. AGEs wirken zytotoxisch auf BHK 21 Fibroblasten und humane SH-SY5Y Neuroblastoma Zellen. Die Toxizität unterschiedlicher Modell-AGEs ist abhängig von verschiedenen Faktoren, u.a. von dem zur Herstellung verwendeten Protein und vom Zucker. Die LD50 der Modell-AGEs korreliert …
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Molecular analysis of La Crosse virus transcription and replication in host cells
… 48 hours after infection of baby hamster kidney (BHK-21) cells. Two predominant 5' nonviral sequences [sequence A (S'-CAGTTACGTT- 3') and sequence B (5'-TTGGTCGTCATCG-3')] were observed on LAC mRNA. Sequence A comprised 66.0% of sequences observed and sequence B comprised 5.2% of sequences …
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