Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"BEAS-2B"”.
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MUTAGENIC EFFECTS OF CHROMIUM (IV) ON HUMAN ENDOTHELIAL AND LUNG EPITHELIAL CELLS
… (HUVEC) and human bronchial epithelial cells (BEAS-2B) were investigated through morphological, cell viability, gene expression, and cDNA sequencing assays. The cell morphology showed both HUVEC and BEAS-2B cells suffered significant but different degrees of damage and demonstrated features …
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Bone Morphogenetic Protein Signalling in Adult Lung Epithelial Cells
… included the transformed bronchial cell line, BEAS-2B, the adenocarcinoma-derived cell line, A549, and normal primary murine airway epithelial cells (MAECs). BMP2 and BMP4 inhibited E-cadherin expression in BEAS-2B cells. In addition, BMP4 induced an epithelial-mesenchymal transition (EMT) in …
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The Effect of Phosphodiesterase Inhibitors on the Induction of Gene Expression by Long-Acting beta2-Adrenoceptor Agonists and Glucocorticoids
… plus a PDE4 inhibitor successfully sensitized BEAS-2B airway epithelial cells transfected with a cyclic adenosine 3',5'-monophosphate (cAMP)-response element luciferase reporter to the LABA, formoterol. Furthermore, PDE3 plus PDE4 inhibitors in combination prolonged and/or sensitized the …
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Regulation of cAMP-Dependent Gene Expression in Airway Epithelial Cells: A Transcriptional and Pharmacodynamic Analysis
… genes with disease-relevant functions. In the BEAS-2B airway epithelial cell line and in human primary bronchial epithelial cells (HBEC), the LABAs, indacaterol and salmeterol, promoted robust transcriptional responses. Functional annotation and gene ontology analyses identified genes with both …
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Evaluation of small extracellular vesicular microRNAs as biomarkersfor exposure of nicotine and E-liquids on human lung cells
… treated lung epithelial cell lines A549 and BEAS-2B with E-liquid (with and without nicotine) and commercial nicotine (100μM) for 72hrs. After treatment, sEVs were isolated using the Size Exclusion Chromatography (SEC) method, followed by total RNA isolation. Isolated sEVs were visualized …
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In vitro analysis of volatile organic compounds in search of potential biomarkers of lung cancer
… (MMSE experiment) and normal human bronchial BEAS-2B cells(TD experiment). In addition, the VOC patterns between the growing and confluent cellsof the same cell line were compared for the first time. In the MMSE experiment, seven VOCs were produced and 14 VOCs metabolisedexclusively by the …
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NOVEL THERAPEUTIC STRATEGIES IN LUNG CANCERSTRONG BIOLOGICAL BIAS FOR ALK INTRON 19 BREAKPOINTS IN NON-SMALL LUNG CANCER
… with the EGFR inhibitor osimertinib) and BEAS-2B cells by CRISPR/Cas9. High-throughput genome-wide translocation sequencing was performed on engineered PC9 cells. NSG immunodeficient mice were used for in vivo experiments with NIH3T3 and BEAS-2B cells. The retroviral overexpression system …
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IgE-reactivity and proinflammatory cytokine -inducibility of various recombinant house dust mite allergens including novel rDer f 4
… In this study, IL-6 and IL-8 production in BEAS-2B cells (bronchial epithelial cell-line) were examined by challenging of the recombinant mite allergens. rDer f 4, rDer f 5, rDer f 10 and rDer f 21 induced IL-6 or IL-8 secretions in BEAS-2B. In particular, rDer f 21 could trigger IL-6 and …
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ROLE OF EXTRACELLULAR VESICLES IN THE ASSOCIATION BETWEEN AIR POLLUTION EXPOSURE AND MAJOR DEPRESSIVE DISORDER
… e metodi: cellule umane di epitelio bronchiale (BEAS-2B) sono state trattate con tre tipi di PM fine (ERM-CZ110, NIST2786, e PM Como) a diverse concentrazioni, e l’effetto di questi trattamenti sulla vitalità cellulare è stato valutato mediante saggio MTT e marcatura con ioduro di propidio. Le …
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Development of Aerosolizable mRNA Incorporated Chitosan Nanoparticles for Efficient Lung Delivery
… was studied on A549 (lung adenocarcinoma) and BEAS-2b (lung epithelial) cell lines. Cellular uptake and protein expression were observed using Confocal imaging on the BEAS-2b cell line. Immunogenicity studies on transfected cells revealed a dosedependent release of Inflammatory cytokines …
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Localization of Human Prostaglandin E2 Receptors in Polarized Epithelial Cells
… these receptors in human bronchial epithelial (BEAS-2B) cells were quite similar to the MDCK cells, suggesting that receptor localization is not dependent on cell type. Additionally, in an attempt to locate structural motifs responsible for apical or basolateral localization, receptor chimeras …
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Synthesis and evaluation of novel heterocyclic compounds as anticancer agents
… ability tosuppress the growth of A549, U-2 OS, BEAS-2B, Saos-2, Hep-G2, and A204 mammaliancell lines by MTT assay, and flowcytometry. 3-Hydroxy-4-methoxy-N-(2-((3,4,5-trimethoxybenzyl)amino)pyridin-3-yl)benzenesulfonamide, was the most potentcompound evaluated with an IC50 of 1.15µM in the …
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The Role of Bone Morphogenetic Protein (BMP) Signalling in Lung Cancer and Lung Regeneration
… to transfect BMPR-IB gene into the human BEAS-2B cell line, which is devoid of endogenous BMPR-IB expression. The effects of BMPR-IB expression on proliferation, BMP signalling, and differentiation were assessed. BMPR-IB expression caused a reduction in viable cell number, an increase in …
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Recommendations for selection efforts to improve the therapeutic quality of Echinacea angustifolia crops in British Columbia
… IL-8) secretion in human bronchial epithelial (BEAS-2B) cells challenged with rhinovirus. When used in isolation however, pure tetraene alkamide showed a significant inhibitory effect on secretion, thereby further supporting the use of high alkamide production as a selection criterion for …
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Fusion Proteins as Selective Inducers of Constitutive Activity and Tools to Discriminate the Biased Signaling Profile of β2 Adrenoceptor Ligands
… human lung non-tumorigenic epithelial cell line, BEAS-2B cells. This immortalized human bronchial epithelial cell line has immunomodulatory properties through cytokine release mediated by β2AR stimulation. Our findings suggest that each signaling pathway of the β2AR is biased towards either the …
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Aerosolizable Drug-Loaded SPIONs for Treatment and Diagnosis of Pulmonary Tuberculosis
… delivery. Cytotoxicity assays in A549 and BEAS-2B cells confirmed biocompatibility over 72 hours. The nanoparticles' extracellular antibacterial activity, coupled with their efficient uptake by macrophages, confirms that nanotechnology-enabled delivery can simultaneously enhance the safety, …
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The effect of environmental stress and selective glucocorticoid receptor modulators on chicken and human leukaemia cells
… on proliferation of normal lung bronchial cells Beas-2b and MCF-C7, whereas T and THCL showed some stimulatory effect on HACAT cells proliferation. Compounds exerted selective and differential effects on cell cycle progression, apoptosis and caspase-8 enzyme activation. Normal peripheral blood …
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Development of a methodology for applying shear stress to suspended mammalian cells
… screening, the bronchial epithelial cell line (BEAS-2b) was trained with the shear loading system. Cell integrity properties before and after the stress imparting were evaluated and compared. Cellular deformation was captured during the process with a high speed camera to relate the cellular …
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Development and characterisation of air-liquid interface exposure systems for cell and tissue cultures
… concentration. Biological testing using A549 and BEAS-2B cells also showed concentration-dependent toxicity. Additionally, the InHALES system is described, created to offer a more precise exposure platform in which the inhalation-exhalation cycle can be modelled and controlled. The research …
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Responses of respiratory system cells in vitro and in vivo to petrochemical combustion-derived ultrafine particles
Environmental contamination with airborne particles has been a human health concern for many years. Epidemiologic studies in urban communities have linked ambient particle exposure to various health effects, including chronic obstructive pulmonary disease, lung cancer, and several cardiovascular …
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