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Showing 1 to 7 of 7 for “"BCECF"”.
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Die pH-Regulation isoliert in vitro perfundierter Kolonkrypten der Ratte
… des pH-empfindlichen Fluoreszenzfarbstoffes BCECF untersucht. Die Perfusionstechnik erlaubte es, Transportprozesse eindeutig der luminalen oder basolateralen Membran zuzuordnen. Da im Dickdarm hohe Konzentrationen schwacher organischer Säuren und Basen vorliegen, wurde untersucht, welchen …
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Intracellular pH, the Proximate Signal for Cell Volume Changes that are Mediated by the Actin Cytoskeleton
… taste receptor cells (TRCs) loaded with BCECF in vitro. Ammonium pulses caused a brief, reversible alkalinization in pHi and induced cell swelling. Sodium-acetate pulses reversible decreased TRC pHi and induced cell shrinkage. Removal weak acids and return to Control Ringer's solution …
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Die Regulation des Na+2Cl-K+-Cotransporters in der basolateralen Membran der Rattenkolonkrypte
… wurde die NH3/NH4+-Puls Technik zusammen mit der BCECF-Fluoreszenzmessung verwendet. Dabei stellt die langsame Azidifizierung des Zytosols in der zweiten Phase des NH3/NH4+-Pulses ein Maß für den NH4+-Einstrom in die Zelle dar. <br>Vorexperimente ergaben, daß neben dem Na+2Cl-K+-Cotransporter auch …
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Flowzytometrische Erfassung der Phagozytose schwarzer Hefen durch humane Granulozyten
… in standardized media. Using fluorescently (BCECF-AM) labelled yeasts we had been able to distinguish yeast-cells that had been phagocytised (or adhered to) by PNG from free (non-phagocytised) yeast cells. The further quantitative analysis focused on human neutrophils participating in …
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Regulation of Intracellular pH and Plasma Membrane Potential in Trypanosoma Cruzi and Trypanosoma Brucei
… utilizing the pH sensitive fluorescent dye BCECF and the potentiometric fluorescent dye bisoxonol. pH i in T. cruzi was found to be regulated primarily by a H+-ATPase with support from a Cl- conductive channel in all stages and an inwardly directed K+ channel in the mammalian stages. The …
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Sodium entry pathways in isolated goldfish hair cells
… measured using the fluorescent probes SBFI and BCECF, respectively. The total steady-state Na⁺ entry, determined by measuring the initial rate of increase in [Na⁺]i during inhibition of Na⁺,K⁺-ATPase, was 9 mM/min, a rate higher than in many other epithelial cells involved in rapid ion …
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Regulation of acid-stimulated H+ secretion by Pyk2 and MAPK signaling pathways in the outer medullary collecting duct
… SB203580. The pH-sensitive fluorescent probe BCECF-AM was used to assay H+ transporter activity. The activity of H+ transporters was measured as the rate of intracellular pH recovery after an NH4Cl pre-pulse. We show that Pyk2 is endogenously expressed and activated by acid pH in mouse-derived …