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Showing 1 to 13 of 13 for “"BAR domain"”.
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Linking WRP/srGAP3 to the Cognitive Deficits in 3p- Syndrome and Its Role in the Regulation of Dendritic Filopodia Formation
… WRP is a member of the srGAP family of Rho GAP domain containing proteins, which share a characteristic domain organization and are expressed throughout the brain. Recently, one of the members of this family was found to contain a newly described inverse F-BAR (IF-BAR) domain of unknown function …
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Computational investigations of protein dynamics and its implication in cellular functions: two cases on membrane sculpting by protein complexes and molecular origin of Parkinson's disease
… approaches: membrane sculpting by F-BAR domains and transient β-hairpin structure in α-synuclein. Interplay between cellular membranes and their peripheral proteins drives many processes in eukaryotic cells. Proteins of the Bin/Amphiphysin/Rvs (BAR) domain family, in particular, play …
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Computational Investigation of Protein-Membrane Systems Involved in Cellular Trafficking
… membrane-bound compartments. To overcome the barrier the membranes pose for cellular trafficking between the outside and the inside, or between different compartments of the cell, nature has evolved elaborate ways of transporting targets across the membranes, utilizing specially designed …
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Cell Biology of the ICA69 protein family in Neurosecretory cells
… neurosecretion. Interestingly, ICA69 contains a BAR domain, present in several proteins involved in intracellular transport. The BAR domain functions as a dimerization motif, provides a general binding interface for different types of GTPases, and is a membrane binding/bending module. Its …
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Self-assembly of filopodia-like structures on supported lipid bilayers
… is not a result of templating by PI(4,5)P2 microdomains but instead by the self-organization of tip complex assembly on uniform PI(4,5)P 2-enriched regions. BAR domain protein toca-1 recruits N-WASP then the Arp2/3 complex and actin assembly follow. Elongation proteins Diaphanous-related formin, …
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Coarse-Grained Molecular Dynamics Simulations of Macromolecular Complexes
… to investigate concerted action of multiple BAR domain proteins, which sculpt curved membranes in cells. Various arrangements of BAR domains on the membrane surface are found to lead to distinct membrane curvatures and bending dynamics. Also, a study on a much larger scale is performed, where …
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Lipid and Protein Sorting by, and Generation of, Membrane Curvature in Model Systems
… the two-dimensional thermal fluctuations of domain boundaries. These parameters are essential determinants of domain stability, and their quantification will yield an increased understanding of the physical processes responsible for aspects of lateral phase separation. Furthermore, …
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Linking Architecture and Function of Endoplasmic Reticulum - Plasma Membrane Contact Sites
… methods for this study were rst established on BAR domain- containing proteins at the PM. These methods, in combination with cryo-focussed ion beam milling, were subsequently used to visualise ER-PM contact site protein organisation in situ. The tricalbins are lipid transfer proteins integral to …
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Understanding Membrane Curvature Sensing
… to track fluorescently labelled lipid-binding domains bound to liposomes of different sizes moving freely in solution. The comparison of the size distribution of the total liposomes with the fluorescently labelled population allowed me to determine their curvature preferences. To validate the …
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Model Systems For Studies At The Plasma Membrane: Investigating Lipid Phase Behavior And Protein-Mediated Membrane Remodeling
… mixture of lipids and proteins. Functional domains ("lipid rafts") that arise from nonrandom mixing of membrane components are believed to be important in governing the spatial organization of lipids and proteins. Proper compartmentalization of lipids and proteins is vital to facilitate …
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Engineered Cytoskeletal Arrays Reveal Mechanisms Of Membrane Transport And Tubulation
… membrane, sorting cargo to distinct subcellular domains. Next, we investigated the interplay between Myo1c and kinesin-1 on deformable giant unilamellar vesicles (GUVs) at physiologically relevant micropatterned arrays of sparse microtubules crossing dense actin filaments. We found that the lipid …
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Dissecting Synaptic Vesicle Endocytosis
… phosphatase, calcineurin. Removal of the F-Bar domain protein syndapin 1, a dynamin 1 binding partner that requires dynamin 1s dephosphorylation, also distorted the acceleration. We examined the setpoint of synaptic vesicle endocytosis between boutons, between cells, and under different …
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Molecular mechanisms of Sorting nexin-9 in membrane triggered actin polymerisation
Sorting nexin-9 (SNX9) is a SH3-PX-BAR domain-containing adaptor protein, func- tionally implicated in regulating actin polymerisation in clathrin mediated endocy- tosis and filopodia formation. Alterations in SNX9 expression correlate with several stages of breast cancer metastasis, and poor …