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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"BAPTA"”.
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A possible role for intrinsic calcium buffers in the long- term adaptation of crayfish neurons
… terminals to exogenously applied calcium buffer, BAPTA-AM, which decreases transmitter release (Robitialle and Charlton, 1992). If LTA increases intrinsic Ca2+-buffering, the membrane permeant form of BAPTA should have less effect on adapted nerve terminals than on controls. Experiments are …
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Hair Bundle Stiffness in the Turtle Utricle: Structural and Regional Variations
… removal with exposure to the Ca²⁺ chelator BAPTA and the protease subtilisin. BAPTA treatment reportedly breaks tip, kinocilial and ankle links while subtilisin breaks the shaft and ankle links. Following BAPTA and subtilisin treatments, bundle stiffness reduced by 65 ± 10% and 63 ± 11%, …
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Calcium, actin and the spectrin skeleton: Responses to swelling and shrinking perturbations.
… the levels which would stimulate exocytosis. In BAPTA loaded neurons, resting (Ca) $\rm\sb{int}$ was about one-half its normal value and did not change with osmotic perturbations. BAPTA was therefore effective at chelating neuronal intracellular calcium. BAPTA loaded neurons, like control neurons …
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Mesures des concentrations totales en calcium et en zinc au niveau de tissus biologiques (cerveau et muscles squelettiques)
… Cette technique est basée sur les propriétés du BAPTA dont le spectre d’absorbance UV est modifié en fonction de la quantité de Ca2+ liée (Tsien, 1980; Biochemistry, 19(11): 2396-2404). Qian et Colvin, 2015 (Journal of General Physiology 147(1): 95-102) ont souligné que la liaison du BAPTA au …
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Funktionalisierte OS(T)K-Stäbe und Sensorfluorophore zur optischen Sensorik
… basierend auf einem DBD-Fluorophor und BAPTA, zur Messung der intrazellulären Calcium-Konzentration dargestellt. Für die Synthese von molekularen Sonden für abstandsabhängige Messungen wurden verschiedenste einfach- und doppelt-markierte OS(T)K-Stäbe entwickelt und spektroskopisch …
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The regulation of hypoxia-responsive gene expression by hydroxyl radicals and intracellular calcium
… während der intracelluläre Calcium Chelator BAPTA-AM HIF-1a nur transient durch Protein-Stabilisierung induziert. Zusammenfassend regulieren beide, A23187 und BAPTA-AM, die Expression des HIF-1 Zielgens PAI-1 hoch.
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Synthesis of Fluorescent Molecules and their Applications as Viscosity Sensors, Metal Ion Indicators, and Near-Infrared Probes
… bioimaging experiments. In Chapter 2, a new BAPTA (O,O*-bis(2-aminophenyl)ethyleneglycol-N,N,N*,N*-tetra acetic acid) and BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)-based calcium indicator, BAPBO-3, is reported. A new synthetic route was employed to simplify both synthesis and …
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Calcium-dependent mechanisms in the chicken retina
… -aminophenonxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA) in the recording pipette, MCU inhibition caused an increase in the current amplitude indicating that the fast buffering capability of BAPTA minimizing the Ca2+-dependent inactivation of the channels, revealing a Ca2+-dependent enhancement, …
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Studies on transient receptor potential vanilloid subtype 1 (TRPV1)-mediated degeneration of mesencephalic cellsin vivo and in vitro
… AM251 or the intracellular Ca2+ chelator BAPTA/AM. We also found that CAP or AEA increased mitochondrial cytochrome c release as well as immunoreactivity to cleaved caspase-3, and that the caspase-3 inhibitor z-DEVD-fmk protected DA neurons from CAP- or AEA-induced neurotoxicity. …
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An investigation into the role of cytosolic free Ca2+ in Salicylic acid mediation of disease resistance in Arabidopsis
… response with the extracellular Ca2+ chelator BAPTA causes a reduction in NPR1 mRNA levels. We have isolated two putative mutants that are defective in their ability to produce a SA-induced [Ca2+]c increase. Characterisation of these mutants is underway and will prove invaluable in identifying …
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Cell biology of settlement and adhesion processes of biofouling algae
… Biolistic delivery of dextran Oregon Green BAPTA-1 and Texas Red enabled ratiometric imaging with a 5-fold greater response to Ca\(^{2+}\)-ionophores than AM-ester Ca\(^{2+}\) indicators. During settlement, zoospores exhibited both localised and diffuse increases in cytosolic calcium …
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Kurz- und Langzeitplastizität an einer hippokampalen Körnerzell-Korbzell-Synapse
… Moosfaserkollateralen. <br>Der Calcium-Chelator BAPTA beeinflußte in einer Konzentration von 10 mM <br>weder PTP noch LTP, attenuierte jedoch in einer Konzentration von 30 mM <br>in der postsynaptischen Korbzelle die LTP. Sowohl ein Adenylylzyklase-Aktivator <br>als auch ein Proteinkinase-C …
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Characterization of Palmitic Acid Induced Lipotoxicity in Schwann Cells
… of iSC cultures with the calcium chelator BAPTA-AM resulted in an increase in cell viability in iSC cultures. In addition, treatment with antioxidant MCI-186 reduced oxidative stress and increased cell viability in both euglycemic and hyperglycemic SC exposed to PA. Furthermore, PA-LTx was …
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Characterization of Cytotoxicity against Cancer Cells induced by Human Monoclonal Antibody Specific to TRAIL-R1
… cell death, Jurkat cells were pretreated with BAPTA/AM (intracellular Ca++ chelator) before treatment of DR4-4 Fab. To determine change of mitochondrial membrane potential, Jurkat cells were treated with DR4-4 Fab and stained with …
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Cyclic AMP and the Induction of Reactive Astrogliosis
… levels (as measured by Oregon Green conjugated BAPTA), and the relation of this to PKA is currently under investigation. On the basis of these results, it appears that induction of cAMP production in F98 cells results in a PKA-dependent increase in phosphorylation of cytoplasmic and nuclear …
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Antiproliferative activities of Euphorbia bicolor (euphorbiaceae) and Maclura pomifera (moraceae) extracts on estrogen receptor-positive and triple-negative breast cancer cell lines
… with capsazepine and using the calcium chelator BAPTA-AM rescued cell viability, confirming the role of TRPV1. The extracts activated multiple apoptotic pathways, including mitochondrial, ERK-induced, and endoplasmic reticulum stress-mediated apoptotic mechanisms. Key apoptotic markers such as …
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Higher order chromatin degradation induced by hydrogen peroxide in glial cells
… -tetraacetic acid tetra(acetoxymethyl) ester (BAPTA/AM) prior to H 2O2 exposure inhibited HOCD in a dose dependent manner. However, neither removing extracellular calcium, nor blocking calcium release from mitochondria and endoplasmic reticulum with …
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Mechanisms of neuronal integration in adrenomedullary sympathetic preganglionic neurons.
… was suggested by caffeine (10mM) decreasing, BAPTA-AM (15muM) and calcium free aCSF increasing the junctional conductance. Electrical stimulation of the descending fibres in both the ipsi- and contralateral funiculi evoked fast EPSPs in all AD-SPN that were mediated by both NMDA and non-NMDA …
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Reactive Oxygen Species-Induced Necrotic Cell Death
… contrast, chelation of intracellular Ca2+ with BAPTA-AM completely abrogated TGHQ-induced necrotic cell death. Ca2+ chelation not only prevented the collapse in the mitochondrial potential but also attenuated PARP-1 hyperactivation. Conversely, inhibition of PARP-1 modulated TGHQ-mediated …
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Molecular Mechanisms Underlying Differential Regulation of Platelet Dense Granule Secretion by Protein Kinase C delta
… When calcium pathways are blocked by dimethyl-BAPTA, AYPGKF-induced aggregation in PKCδ null mouse platelets and in human platelets pretreated with (δV1-1)TAT, was inhibited. In a FeCl3-induced injury in vivo thrombosis model, PKCδ-/- mice occluded similar to their wild-type littermates. Hence, …
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