Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"B-cell line"”.
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A lentiviral screen for novel regulators of IL-7R[alpha] in a pre-B cell line
… this receptor is essential for murine T and B cell development as well as human T cell development. IL-7R[alpha] signaling is also responsible for homeostatic proliferation of T cells in lymphopenic hosts, as well as maintenance of naïve and memory CD8+ T cells in the periphery. A number of …
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Proteins mediating somatic hypermutation and class switch recombination at the immunoglobulin locus
Summary Antibodies are produced by B cells before antigen contact. A given B cell synthesizes antibodies of one specificity only. However, the large number of B lymphocytes allows for a large diversity, which stands ready to meet the antigenic universe. Upon contact with the cognate antigen, the B …
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Molekularbiologische und funktionelle Charakterisierung neuer "Chicken Ig-like" Rezeptoren
… receptor families on myeloid and lymphoid cells in man and mouse have both inhibitory and activating receptors with important functions in the regulation of the immune system. Due to the lack of information regarding these receptors in other vertebrates, in the present thesis seven new …
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Cll Metabolism Is Regulated By Prognostic Factors, Modulated By Stroma and Abrogated By Pi3K Inhibition
… by the relentless accumulation of mature B cells has been little explored. Bone marrow stromal cells provide a survival benefit to CLL cells, in part through PI3K/AKT pathway. Compared with proliferative B-cell lines, metabolic fluxes of oxygen and lactate were low in quiescent malignant B …
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Characterization of downstream target genes regulated by ABF-1 in different states of B cell development
… nodes, and appendix, as well as transformed B cells lines infected with Epstein-Barr virus. This study investigates the role of ABF-1 in regulating downstream target genes in the human mature B cell line RAJI, as well as the plasma cell line, ARH-77. Quantitative real-time polymerase chain …
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Maus-Herpesvirus-68-(MHV-68)-Infektion : Modell für die Epstein-Barr Virus-Infektion des Menschen
… there was to develop a MHV-68-permissive cell line which is suitable for in vitro investigations. The following results were achieved:- infection of the CNS succeeded after intranasal application of MHV-68- detection of viral antigens in the CNS and other organs succeeded by means of …
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Expression and function of the αVβ5 integrin during human B lymphopoiesis
… interaction sustains proliferation of the pre-B cell line SMS-SB. This thesis describes further investigation into the role of αVβ5 integrin during human B cell development. B cell development in the bone marrow involves stepwise maturation of progenitor cells through different defined stages. A …
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Experimental methods by optical trapping for investigation of leukocyte cell rolling mechanics
… the mechanical behavior of the leukocyte cell membrane as it undergoes cell rolling. Membrane tethers extracted from the cell during rolling function to slow the cell down and to maintain contact with the endothelial wall. Understanding the dynamic behavior of the leukocyte membrane during …
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The Indirect Effect of BVDV on Immune Suppression: The Role of Infected Macrophages in Lymphocyte Apoptosis
… the fresh peripheral blood lymphocytes or BL-3 B-cell line. We found that only supernatant from the high virulent BVDV strain induced lymphocyte apoptosis. Furthermore, direct infection of lymphocytes with the same virulent strain did not induce significant apoptosis. We investigated the …
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In vitro diversification of chimeric human/chicken antibodies
Cells that constitutively diversify their immunoglobulin genes can be used for selection of novel antibodies and refining affinities and specificities of existing ones (Cumbers et al., 2002). The chicken derived DT40 B cell line has been adapted for the expression and diversification of humanized …
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Characterising the function of CDK5RAP2 in the vertebrate centrosome
… microtubule organising centre in vertebrate cells. CDK5RAP2 is a human protein that localises to the centrosome. At the start of this thesis work, the function of CDK5RAP2 was uncharacterised. Significantly, cdk5rap2 is one of several centrosomal genes that are mutated in the developmental …
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Die Bedeutung von Zinkionen für mononucleäre Leukocyten
… important influences of zinc on mononuclear cells were examined.For the first time, a direct stimulation of cells by zinc could be shown, which was dependent on zinc concentration and could not be induced by other cations. Even in physiological doses, zinc interacts significantly with …
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The Role of Foxo Transcription Factors in B Cell Development and Activation
… system depends on a diverse, self tolerant B cell repertoire. Mature B cells distributed throughout secondary lymphoid organs respond to antigenic stimuli by dividing and differentiating into plasma cells and other effector cell types. Signaling from the B cell receptor (BCR) plays a critical …
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Mechanism of Mismatch Repair Induced Mutagenesis in Somatic Hypermutation
B cells produce a diverse array of antibody specificities that are of low affinity during the initial phase of a humoral immune response. However, somatic hypermutation of the rearranged V region in the immunoglobulin locus generates new antibody affinities, accompanied by the selection of B cells …
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The role of the adapter protein TSAd in T cell activation
Introduction: T cells are a part of the adaptive immune system, which is designed to attack pathogens in the body. T cells recognize antigen presented by HLA molecules on the surface of antigen presenting cells (APC) through their T cell receptor. Conjugation formation between T cells and APC turns …
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Role of O-GlcNAc in the Vertebrate Secretory Pathway
… we used a combination of chemical, biochemical, cellular and genetic approaches to demonstrate that site-specific O-GlcNAcylation of COPII proteins mediates their protein-protein interactions and modulates cargo secretion. We demonstrate that individual O-GlcNAcylation sites of SEC23A are …
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Identification of Metabolic Markers as Potential Early Predictors of PFAS- Induced Immunotoxicity
… acid, PFOS) suppress both the T-cell-dependent (TDAR) and T-cell-independent antibody responses (TIAR). Previous studies have demonstrated that exposure to PFAS is associated not only with suppression of the TDAR but also with changes in overall B cell numbers, suggesting …
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Charakterisierung des Zytokins BAFF als wichtiger Regulator der B-Zellfunktion beim Haushuhn
… chicken homologue of the mammalian cytokine “B cell activating factor belonging to the TNF family” (BAFF) in a chicken EST-database. The cDNAs encoded for the complete open reading frame of chicken BAFF (chBAFF) including a transmembrane domain and a potential furine cleavage side. ChBAFF shows …
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Aqueous extracts of Dodonaea viscosa induce potent and selective cytotoxicity in DLBCL cells
… among both males and females. Diffuse large B cell lymphoma (DLBCL), an aggressive B-cell derived cancer is the most prevalent cancer within the NHL group of cancers. DLBCL can be grouped into two main subtypes, namely the germinal-centre B cell (GCB) and the activated-B cell (ABC) subtypes, …