Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"B-catenin"”.
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INVESTIGATING ALTERNATIVE SPLICING REPROGRAMMING REGULATED BY THE E-CADHERIN/B-CATENIN AXIS
… this dysregulation, pinpointing E-cadherin and β-catenin as central players in myosin VI AS regulation. β-catenin's nuclear translocation induces myosin VIshort expression, while its depletion rescues myosin VIlong. Furthermore, we uncovered β-catenin's broader involvement in AS oncogenic …
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Investigating the Anti-Tumor Effects of Two Novel B-Catenin Inhibitors in Ovarian Cancer Cells
… challenge in treating ovarian cancer. The Wnt/-catenin pathway, which plays a critical role in developmental and physiological processes, have been indicated to contribute to EOC development. Aberrant activation of this pathway was reported to promote cancer stem cell self-renewal, metastasis, …
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Signaling Pathway Inhibitor Library Screening Reveals B-Catenin/TCF4 as a Novel Telomerase Regulator in Cancer cell lines
… for some of the major types of cancer. ?-catenin/TCF4 complex was identified as a novel TA regulator from the screen and was later found to inhibit TA via transcription regulation of hTERT (human TERT). Activation of the Wnt pathway either by Wnt ligand (Wnt-3a) or LiCl (activates Wnt …
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Novel interactions and functions of the Adenomatous polyposis coli protein
… to its role in Wnt signalling where it limits b-catenin levels in the absence of wnt ligands.<br/><br/>The aim of this project was the identification of additional proteins that bind to and are regulated by APC. I applied an untargeted global approach using immunoprecipitation of endogenous, …
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Charakterisierung des mit der Alzheimer Krankheit assoziierten Genprodukts Presenilin 1 und Interaktion mit beta-Catenin
… als auch intrazellulär wichtigen Protein bCatenin. Zur Verifizierung der Interaktion wurden die in dieser Arbeit hergestellten Antikörper verwendet. Mit Hilfe von Koimmunpräzipitationsexperimenten ist es gelungen, sowohl die Interaktion zwischen PS1-NTF und PS1-CTF nachzuweisen als auch die …
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Wnt-/beta- Catenin Zielgene der frühen Mausentwicklung
… entwicklungsrelevante Zielgene der Wnt-/b- Catenin- Kaskade identifiziert. <br>Es wurde ein Zellkultursystem etabliert, mit dem es möglich war, unter einfachen experimentellen Bedingungen, die Wnt- Kaskade in Zellen induzieren zu können. Für das benutzte Zellkultursystem aus Embryonalen …
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Mechanisms Regulating The P120-Catenin/Kaiso Pathway
… in cancer and developmental biology, with β-catenin being a key canonical component. P120-catenin, which is structurally similar to β-catenin, regulates the expression of certain Wnt target genes, relieving repression conferred by the POZ/ zinc-finger transcription factor Kaiso. In my first …
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Dissecting Pluripotency and Mammalian Embryonic Development via Droplet Microfluidics
… I then identified plakoglobin, a homologue of b-catenin, as one of the strongest upregulated proteins upon microgel encapsulation. However, molecular functions of plakoglobin in embryonic stem cells remain largely elusive. To investigate plakoglobin’s potential role during naïve pluripotency, I …
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Synthetic Essentiality of Tdo2 In Apc-Mutated Colorectal Cancer
… <p>Mechanistically, APC loss results in TCF4/b-catenin-mediated activation of</p> <p>TDO2 gene transcription. TDO2 in turn activates the Kyn-AhR pathway</p> <p>which boosts glycolysis, promotes proliferation, and upregulates CXCL5</p> <p>which recruits macrophages into the tumor microenvironment …
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Modulating ADAM-10 activity and expression in cervical and oesophageal cancer cells
… in epithelial cell markers (E-Cadherin, B-Catenin) and a decrease in mesenchymal marker expression (Vimentin) post treatment with the compounds tested strongly suggested that ADAM10 plays a role in mesenchymal cell transition. These results suggest that ADAM10 activity is necessary for the …
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EPIGENETIC MECHANISMS OF POLYCOMB REPRESSIVE COMPLEX 1 IN INTESTINAL LINEAGE COMMITMENT
… tissue homeostasis by preservation of the WNT/B-catenin activity (4). However, the contribution of PCGFs in adult intestinal homeostasis is unclear. To address this, we evaluated the role of six different PRC1 subcomplexes in transcriptional repression maintenance in intestinal cells by using …
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Casein Kinase I Transduces WNT Signals
… signaling. First, CKI expression stabilized b-catenin protein levels, a hallmark of Wnt signaling. Second, CKI induced the expression of Siamois and Xnr3, direct transcriptional targets of Wnt signaling. Finally, CKI expression dorsalized the ventral marginal zone in Xenopus, inducing markers …
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Significance of Increased Tissue Transglutaminase In Hormone Refractory Prostate Cancer
… of epithelial markers (such as E-cadherin, b-catenin). Taken together, these results suggested a new function for TG2 and revealed a novel mechanism that is responsible for the progression of prostate cancer to the aggressive hormone-refractory phenotype.</p>
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Hepatitis C Virus-Induced Hepatocellular Carcinoma: Cancer Stem Cell and Gene Therapy
… c-Myc, and c-Src, and activation of b-catenin pathway in the absence of Wnt ligand. The result presented here implicates a novel cellular internal ribosome entry site (IRES) element in the c-Src mRNA responsible for the overexpression of c-Src proto-oncogene under stressed conditions …
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Elucidating the Role of Plakoglobin in Embryonic Stem Cell Culture
… independence of Plakoglobin from its homologue b-catenin was tested with knock-out cell lines, which showed the support of naïve pluripotency similar to the parental overexpression cell line. (4) The role of Plakoglobin in the other two states of pluripotency – formative and primed – was tested. …
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Ancient origins of the chordate forebrain: Conserved patterning of the anterior neuroectoderm in amphioxus
… and hemichordates, are regulated by Wnt/b-catenin signalling and are active in the anterior neuroectoderm that forms the larval brain. As a comparative system, I also characterize the development of the apical organ in an understudied group of echinoderms, the crinoids. To follow the fate …
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Effect of the Insulin-like Growth Factor (IGF) Axis on the Transport Properties of Endothelial and Epithelial Cells In Vitro
… levels of the junction components E-cadherin, b-catenin, and occludin. The differences in parental and IGF-I secreting cells was not due to extracellular stimuli since inclusion of IGF-I, IGFBP-3, or co-culture with SV40-IGF-I cells did not alter the barrier properties of parental cells, …
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Molecular biology and biochemistry of brain tumours
… was also determined. Finally, defects in the ß-catenin gene, an important component of the developmental pathway, in a sub-set of craniopharyngiomas were investigated. Reverse transcription-polymerase chain reaction (RT-PCR), restriction digestion analysis and direct sequencing demonstrated …
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The role of Wnt signalling during embryonic salivary gland development
… formation. To locate the sites of active Wnt/p-catenin signaling, we used Axin2LacZ mice and found that the pathway is active from early stages of salivary gland development in the mesenchyme and at later stages in the ductal epithelium corresponding to the time of branching morphogenesis and …
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Osteogenesis During Early Healing Around Titanium and Titanium Zirconium Implants: Immunohistochemistry and mRNA Expression of Bone Markers in Miniature Pigs
… was foreseen for IHF analysis. The presence of B-catenin, Runx2, Osteopontin(Opn), and Osteocalcin(Ocn) were measured by IHF. RNA was isolated from two animals at 2 weeks; Osterix(Osx), Opn and Ocn mRNA expression levels were evaluated for osteoblasts differentiation by QRT-PCR. <strong>Results: …
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