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Showing 1 to 15 of 15 for “"B-Galactosidase"”.
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ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΜΕΛΕΤΗ ΙΔΙΟΤΗΤΩΝ ΜΙΑΣ Β-ΓΛΥΚΟΣΙΔΑΣΗΣ ΚΑΙ ΜΙΑΣ Β-ΓΑΛΑΚΤΟΣΙΔΑΣΗΣ ΑΠΟ ΣΠΕΡΜΑΤΑ ΚΡΙΘΑΡΙΟΥ. ΕΦΑΡΜΟΓΗ ΤΩΝ ΕΝΖΥΜΩΝ ΣΤΗΝ ΥΔΡΟΛΥΣΗ ...
TWO ENZYMES A B-GLUCOSIDASE AND A B-GALACTOSIDASE WERE ISOLATED AND CHARACTERIZED FROM BARLEY SEEDS. THE B-GLUCOSIDASE IS A SINGLE BASIC POLYPEPTIDE (PI>8,5) WITH A MR OF 53.000 ACTING OPTIMALLY AT PH 4.5-5.0. THE B-GALACTOSIDASE IS COMPOSED OF TWO SUBUNITS WITH A MR OF 42.000 AND 33.000 …
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Structural and Functional Analysis of Secondary Structure Block to mRNA Utilization
… abolishes splicing in vivo and inhibits B-galactosidase production. This is the first demonstration of the splicing inhibitory effect of secondary structures occurring in exons in the absence of splice site sequestration. Our goal is to understand the mechanism by which utilization of this …
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Development of a Rapid Coliphage Assat
… (RCDA), based on the phage-induced release of b-galactosidase from cells of Escherichia coli (Ijzerman, M., J.O. Falkinham III and C. Hagedorn. (1993) [A liquid, colorimetric presence-absence coliphage detection method. J. Virol. Meth. 45:229-234] was modified to reduce the number of steps …
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Interaction of Bdellovibrio Bacteriovorus and Host Bacteria
… or proteins were being released. No protein or B-galactosidase could be detected in the culture fluid of the two-membered culture. The infected E. coli cells were more permeable than uninfected cells to o-nitrophenyl-B-D-galactoside, the substrate for B-galactosidase, and to the fluorescent dye …
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Untersuchungen zum molekularen Wirkmechanismus der antiinflammatorischen Aktivität von Sesquiterpenlactonen
… von NF-kB-kontrolliertes Luciferase-Gen oder b-Galactosidase-Gen enthielten. In ex-vivo und in-vitro Untersuchungen konnte gezeigt werden, dass SLs und NEM, ein Reagenz zur selektiven Alkylierung von Cys-Resten in Proteinen, die Luciferase-Aktivität durch einen direkten Angriff des Enzyms …
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Characterization of components that increase secretion of recombinant proteins in pichia pastoris
… integration that enabled P pastoris to secrete Pgalactosidase at higher levels than the wild type strain. This study focuses on characterizing the random pREMI-Z mutations in the genomic DNA and examining their secretory phenotype, in hopes of creating a super secretor strain. The ah3 mutant was …
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Gene Therapy for Osteosarcoma : "In Vitro" Characterization of Five Tumor Cell Lines and Development of Treatment Models "In Vivo"
… kinase (HS-tk), human interleukin 2 (IL2). and B-galactosidase (BAG) genes. Each osteosarcoma cell line was stably transduced, and the HS-tk gene effectively conferred ganciclovir (GCV) susceptibility to transduced cells. Each cell line showed a bystander effect (whereby wild type tumor cells are …
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In Vivo Regulation Of Transcription Of The Escherichia Coli Rna Polymerase Genes Rpo(b) And Rpo(c)
… cloned fragment was examined by measuring the b-galactosidase level in a lysogen carrying a transcriptional fusion phage. Two strong promoters, rplKp and rplJp, were identified along with a weak promoter, rplLp. A transcriptional attenuator, atn, located between rplL and rpoB, terminated 60 …
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Haematopoietic progenitor cells in carotid disease
… and by staining for senescence associated B-galactosidase (SA-Bgal). HPC number and function was then measured in 62 patients undergoing CEA pre-operatively, 1 day post operatively and 6 weeks post-operatively. Restenosis was assessed by duplex scanning at 3, 6 and 12 months. The circulating …
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Regulation of the speC gene encoding ornithine decarboxylase in Escherichia coli by putrescine, spermidine and cAMP
… with cAMP, putrescine, or spermidine, the B-galactosidase activity encoded by the speC’-lacZ fusion was compared to the ODC activity encoded by spec. Only cyclic AMP and putrescine repressed the speC’-lacZ fusion. Cyclic AMP, putrescine, and spermidine all repressed the spec gene. A 444 bp …
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REGULATORY PROCESSES IN ULTRA SLOW GROWING MICROORGANISMS (ESCHERICHIA COLI, BACILLUS POLYMYXA)
… rates of degradation of the inducible enzymes, B-galactosidase and tryptophanase. The activity of this system in phase 3 cells is interpretable as signifying the presence of a chromosome replicating fork on only 38% of the population.</p>
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Role of region 4 of the sigma 70 subunit of RNA polymerase in transcriptional activation of the lux operon during quorum sensing
… from a luxI-lacZ translational fusion via b-galactosidase assays in recombinant E. coli. In vitro work was performed with LuxRDN, the autoinducer-independent C-terminal domain (amino acids 157 to 250) of LuxR because purified, full length LuxR is unavailable. Single-round transcription assays …
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Evaluating the Expression of Angiogenic Mediators in a Mouse Model of Tumor Metastasis
… with the lacZ gene, which expresses B-galactosidase activity. These cells, injected intravenously, form tumor nodules in the lung. A pilot study revealed development of lung nodules in mice nine days after intravenous injection with 105 cells. In a second study, twenty-five 10- week-old …
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Towards Accessible Diagnostics: Developing CRISPR-Based Colorimetric Tools for Low- and Middle-Income Countries
… immobilising an oligo covalently linked to a β-galactosidase (LacZ) enzyme, which is cleaved in the presence of DNA target-activated CRISPR-Cas12a. This method demonstrated a lower limit of detection than standard fluorescent assays while remaining active for four weeks after lyophilisation at …
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Indocyanine Green J-aggregate Nanoparticles for Detection of Senescent Cells
… such as the lysosomal senescence-associated b-galactosidase (SA-β-gal) activity and to colocalize with the lysosome. As senescent cells possess a higher lysosomal mass and amount, we hypothesized that this was the reason for the selectivity of the NanoJaggs (Chapter 5). The evaluation of the …