Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"B-DNA"”.
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Mutagenesis by the primary aflatoxin B₁ DNA adduct in Escherichia coli
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1996.
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Interaction of B-DNA and Monovalent Cations: Theory and Practice in X-Ray Crystallography
… Finally, the structures of two modified B-DNA dodecamers are determined to explore the effect of monovalent cations on B-DNA structure. The modifications in these structures harbor tethered cations that are covalently linked to the DNA in the major groove. In one structure, the tethered …
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The involvement of non-B DNA forming sequences in mediating missense mutations, micro-deletions and micro-insertions in human inherited disease
The involvement of the local DNA sequence features (repetitive elements capable of adopting non-B structures, hotspot motifs, mononucleotide runs and tandem repeats) and epigenetic marks in mediating germline missense and nonsense mutations, micro-deletions and micro-insertions causing human …
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Structural determinants of mutability across cancer genomes
… of this variation. Here the role of alternative DNA structures was investigated across a multitude of whole-genome sequenced cancers. Sequences that are predisposed to fold in alternative DNA structures can be identified by the primary DNA sequence of the human genome and are collectively known …
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A structural study of M-DNA
In alkaline conditions, a complex called M-DNA is formed between a divalent metal ion, cobalt, nickel or zinc, and duplex DNA. The rate of formation and stability of M-DNA is dependent on many factors, including but not limited to temperature, pH, DNA sequence, and metal or DNA concentrations. It …
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DNA and RNA synthesis in leaves during TYMV replication
… during the period of rapid virus production. 6. DNA from the nuclei plus chloroplast fraction of healthy Chinese cabbage leaf has been fractionated into two components by HAP column chromatography: Component A-DNA which represents the bulk DNA of the cell, and component B-DNA, a minor component. …
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Genetic interactions of repriming and translesion synthesis
… It has been previously shown to restart DNA synthesis past replication impediments such as DNA damage or non-B-DNA secondary structures. To probe the genetic interactions of PRIMPOL, genome-wide CRISPR/Cas9 knockout screens were undertaken in the human TK6 cell line. The most interesting …
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Double-Strand Break Repair Pathways In Dna Structure-Induced Genetic Instability
… In the absence of exogenous sources of DNA damage, the DNA structure itself has been implicated in genetic instability. When the canonical B-DNA helix is naturally altered to form a non-canonical DNA structure such as a Z-DNA or H-DNA, this can lead to genetic instability in the form of …
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Localization of metal ions in DNA
M-DNA is a novel complex formed between DNA and transition metal ions under alkaline conditions. The unique properties of M-DNA were manipulated in order to rationally place metal ions at specific regions within a double-stranded DNA helix. Investigations using thermal denaturation profiles and the …
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The Influence of Osmolytes on Nucleic Acid Helical Conformations
… on the transition of helical conformation from B-DNA to Z-DNA. Distinct from the familiar, right-handed B-DNA, Z-DNA is a left-handed double helical structure with its phosphodiester backbone arranged in a pronounced zig-zag pattern. This pattern, unique to Z-DNA is formed from alternating …
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Solution structure of unusual DNA sequences by high resolution NMR spectroscopy
Unusual DNA sequences are known to adopt interesting three-dimensional structures which might have important biological roles. For my thesis, I have tried to study the influence of various degrees of perturbations of DNA backbone, sequences, pH etc. on DNA structure by a quantitative NMR procedure …
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Metallo-Supramolecular complexes and their interactions with biomolecules and cells
… of these assemblies are outlined with a focus on DNA recognition. In chapter 2 the synthesis and characterisation of an array of palladium(II) and platinum(II) tetra-stranded, dinuclear complexes is described. The synthesis of two palladium(II) triangles and a palladium(II) pyramid are also …
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Taxonomy, phylogenetic and biogeographical relationships of African grassland Francolins (Genus: Scleroptila)
… sensli lalo). Mitochondrial cytochrome-b DNA sequence data (±250 b.p.) from 50 individuals and 19 morphological characters extracted from reports in published literature were employed to achieve these aims. These characters were analysed separately and also in combination using maximum …
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DNA topology and Pol II CTD phosphorylation as stepwise regulators of chromatin architecture in differentiating human stem cells
… from heterochromatin, and repositioned through DNA looping. While each of these features of Pol II regulation has been well studied in individual cell types, it remains unclear how a single genome can support hundreds of distinct fates. Combinatorial transcription factor binding explains part of …
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Computational analyses of non-canonical architectural and structural features associated with alternative splicing
… related to atypical exon-intron structures and DNA/RNA conformations. First, I studied a group of extremely small exons, known as microexons (≤30 nucleotides), which were shown to be part of an evolutionarily conserved network of neuronal alternative splicing events that play essential roles in …
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Taxonomy, phylogeny and biogeography of francolins ('Francolinus' spp.) Aves: Order Galliformes, Family Phasianidae
… based on partial mitochondrial Cytochrome-b DNA sequences and Restriction Fragment Length Polymorphisms (RFLPs) also challenged the monophyly of the genus and that of some of Hall's (1963) species groups. These findings suggested that francolins may form at least two distantly related …
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The application of scanning tunneling microscopy (STM) and related techniques to the study of organic and biomolecular targets
… adsorbed onto highly uniform Au/mica films, (b) DNA adsorbates on different substrates prepared by different deposition techniques. In addition, surface preparation techniques have been investigated. Two strongly oxidizing solutions (sulfochromic and piranha acids) have been studied for the …
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The Influence of the Cellular Environment on Z-DNA Formation
… influence of cosolutes on the transition from B-DNA to Z-DNA in model DNA duplexes. Distinct from the familiar right-handed BDNA helical conformation, Z-DNA is a left-handed double helical structure with its phosphodiester backbone arranged in a zig-zag pattern that is unique to Z-DNA. Moreover, …
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Phytochemische und pharmakologisch-biologische Untersuchungen von Arnica montana und Vernonia triflosculosa
… eine starke hemmende Aktivität die NF-kapha-B-DNA-Bindung und auf die IL-8-Produktion.
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