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Showing 1 to 20 of 46 for “"Azotobacter"”.
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Tyrosinase and Melanin Pigmentation in Azotobacter Chroococcum
Made available in DSpace on 2014-12-05T21:45:30Z (GMT). No. of bitstreams: 1 5900534.pdf: 2458013 bytes, checksum: 3302b07bb626ef937514100c5a772253 (MD5) Previous issue date: 1958
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Nitrogen Fixation By Azotobacter Species In Mixed Culture
… accumulate in the medium of pure cultures of Azotobacter vinelandii until levels become high enough to repress nitrogenase synthesis. A. vinelandii and A. chroococcum were grown in mixed culture with yeasts and bacterial soil isolates in a nitrogen free medium. It was theorized that the yeast …
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Mutagenesis of nifE and nifN from Azotobacter vinelandii
The products of nifE and nifN from Azotobacter vinelandii, which are involved in the biosynthesis of the iron-molybdenum cofactor (FeMo-co) co) from nitrogenase, have been analyzed using a variety of mutagenic techniques. NifE was the object of several site-specific, amino acid substitutions that …
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Aerobic Hydrogen Production in Azotobacter vinelandii: Genetics, Physiology, and Optimization.
North Carolina State University Theses Microbiology.
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Characterization and site-directed mutagenesis of NifU from Azotobacter vinelandii
… nifU gene product in nitrogenase maturation in Azotobacter vinelandii. the gene product has been hyperexpressed in Escherichia coli and characterized by various biophysical techniques. Following the initial characterization, site-directed mutagenesis of conserved cysteinyl residues was performed …
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Transporte y asimilación de nitrato en la bacteria aerobia "Azotobacter chroococcum"
… hemos estudiado la asimilación de nitrato en Azotobacter chroococcum; en primer lugar se ha determinado la dependencia del proceso de una fuente de energía. Se ha puesto a punto un sistema artificial que actúa como fuente de energía que permite el consumo de nitrato en ausencia de una fuente …
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The effect of various organic compounds on the growth of azotobacter
… of various organic compounds on the growth of Azotobacter. The study was induced by the theory that the soil contains organic substances which are deleterious to plant growth and which are important factors in influencing soil fertility. It is, therefore, interesting to determine if this …
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Site-directed mutagenesis of the nitrogenase MoFe protein from Azotobacter vinelandii
… the four 4Fe-4S centers (P-clusters) within the Azotobacter vinelandii nitrogenase MoFe protein is presented. Based on interspecies and intersubunit amino acid comparisons of the α- and ß-subunits of the MoFe protein, and the FeMoco biosynthetic proteins, NifE and NifN, four conserved residues …
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Biosynthetic study of enzymes and valuable compounds produced by Psychrobacter and Azotobacter
University of Minnesota M.S. thesis. October 2012. Major: Microbial Engineering. Advisor: Dr. Brett M. Barney. 1 computer file (PDF); vi, 69 pages.
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Aislamiento y caracterización de mutantes del metabolismo del nitrogeno en "Azotobacter vinelandii"
… la regulación del metabolismo del nitrógeno en Azotobacter vivelandii es de gran interés debido a su posible utilización con fines prácticos. En esta tesis se aborda el estudio de esta regulación estableciendo la existencia características
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Reducción enzimática de nitrato a nitrito en azotobacter chrococcum dependiente de ferredoxina
Las reacciones y estructuras de las moléculas que contienen nitrógeno juegan un papel esencial en Bioquímica. Realmente la importancia de los ácidos nucleicos, proteínas, muchos coenzimas y otros compuestos nitrogenadas no puede subestimarse. Debido a que la Tierra contiene una atmósfera rica en …
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Characterization of an altered MoFe protein from a nifV- strain from Azotobacter vinelandii
… encoded by nifV. By deleting the nifV gene from Azotobacter vinelandii we were able to study the role of homocitrate in nitrogenase catalysis. A poly-histidine tail was incorporated into the C-termini of the a-subunit permitting isolation of the homocitrateless MoFe protein by using metal …
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Natural transformation and growth inhibition of azotobacter vinelandii in the presence of adsorbed oxytetracycline
… and inhibition of the soil bacterium Azotobacter vinelandii was investigated. The transformation frequency by both plasmid and chromosomal DNA was not significantly affected by the addition of adsorbed OTC, showing that the presence of OTC does not hinder gene transfer. A higher …
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Azotobacter vinelandii nitrogenase: role of the MoFe protein α-subunit histidine-195 residue in catalysis
… were used to isolate mutant strains of <i>Azotobacter vinelandii</i> that produce altered MoFe proteins where the α-subunit residue-195 position, normally occupied by a histidine residue, was individually substituted by a variety of other amino acids. Structural studies have revealed that …
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Genetic analysis of nifF and nifA and site-directed mutagenesis of nifE in Azotobacter vinelandii
… nifE (FeMo-cofactor biosynthesis) genes from <i>Azotobacter vinelandii</i> were genetically analyzed. The nucleotide sequence of the nifF gene, which encodes a flavodoxin, was determined. Specific mutation strains indicated that in <i>A vinelandii</i> flavodoxin is not the unique physiological …
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Controlled Expression and Functional Analysis of the Iron-Sulfur Cluster Biosynthetic Machinery in Azotobacter vinelandii
… for the controlled expression of genes in Azotobacter vinelandii by using genomic fusions to the sucrose catabolic regulon. This system was used for the functional analysis of the A. vinelandii isc genes, whose products are involved in the maturation of [Fe-S] proteins. For this analysis …
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Azotobacter vinelandii Nitrogenase: Multiple Substrate-Reduction Sites and Effects of pH on Substrate Reduction and CO Inhibition
Mo-nitrogenase consists of two component proteins, the Fe protein and the MoFe protein. The site of substrate binding and reduction within the Mo-nitrogenase is provided by a metallocluster, the FeMo cofactor, located in the a-subunit of the MoFe protein. The FeMo cofactor's polypeptide environment …
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