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Showing 1 to 20 of 53 for “"Axenic"”.
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Growth and root inhibition in Axenic hydrilla verticillata cultures
… and Ward (1981) developed a method for producing axenic cultures from tubers. The process by which axenic cultures were produced involves surface treatment of the tuber with sodium hypochlorite and removal of outer bud scales. Tubers were grown in a mineral salts media containing 15 ug/ml …
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Influence of Trichoderma Harzianum Rifai on growth, chemical compositions and root exudation of axenic tobacco
… tabacum</i> L. 'Virginia Gold') grown axenically in gnotobiotic flexible vinyl isolators, were inoculated after 44 days growth with washed conidia of <i>Trichoderma harzianum</i> Rifai by introducing conidia into the rooting medium at a concentration of 1.08 X 10<sup>6</sup> to 1.44 X …
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The effect of nutrient limitation and oxidative stress on bromoform production from axenic cultures of marine diatoms
… oxidative stress on bromoform production from axenic cultures of marine diatoms.
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Pharmacological studies of C. elegans cultured in axenic media: nicotine and its effects on growth, body morphology, and reproduction
… pharmaceuticals introduced to the system. Liquid axenic media is chemically synthetic, optically transparent, and can be utilized in automated microfluidic devices and closed systems. However, there has been no quantification of changes in morphology, development, reproductive fitness, or lifespan …
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Influence of Penicillium simplicissimum (Oud.) Thom and Penicillium citrinum Thom on growth, chemical composition and root exudation of axenic marigold
Axenic marigold (Tagetes erecta L.) were grown in quartz sand free of organic compounds and one-half strength Hoagland and Arnon nutrient solution plus chelated iron, in individual plant containers, designed for collecting root exudates, inside sterile gnotobiotic flexible plastic isolators. Plant …
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Effects of kinetin on distribution and exudation of free sterols and free fatty acids in Arachis hypogaea L. 'Argentine' under axenic conditions
The effect of kinetin concentrations (10⁻⁶M and 10⁻⁴M) on distribution of free fatty acids and sterols in plant parts and root exudates of 57-day-old peanut plants grown in nutrient solutions under gnotobiotic conditions was studied. Kinetin was applied to the roots in the culture medium five days …
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Observations of the growth of two strains of Trichomonas augusta from amphibians
… the effects of various antibiotics upon axenic cultures, and the third, was to compare division rates in various media which did not contain antibiotics. Since the few axenic cultures which were obtained lasted a maximum of four hours, the emphasis of the problem was shifted to the …
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Assessment of wastewater algae for use in biofuel production
… that under mixotrophic growth (14mM glucose) non-axenic algae accumulated significantly higher concentrations of neutral lipids compared to the same algal strains under axenic conditions. The result of which is thought to be caused by bacteria creating a nutrient deprived media causing the algae …
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Syntrophic relationships between algae and bacteria in a fresh-water stream and in culture /
… species of a pond community were isolated in axenic culture and tests were performed to determine the nature of mixed species interactions. Isolation procedures and field studies indicated that selected strains of Chlorella and Azotobacter were closely associated in their natural habitat. With …
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Understanding The Mechanism and Extent of Vlse Recombination In Borrelia Burgdorferi Using Next-Generation Sequencing
… we were able to detect<em> vlsE</em> variants in axenic cultures of <em>B. burgdorferi</em> as well as co-cultures with mouse skin and heart tissues; these results were compared with those from mice infected for 7 days. Analysis of PacBio single molecule real-time (SMRT) sequencing indicated the …
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Micropropagation of Begonia and a study of genome stability in Begonia rex
… leaves derived from singly and multiply recycled axenic plantlets. No significant difference was found in phenotype or quantitative vegetative characters for any of the populations assessed. The results presented from studies on the development of broad spectrum media for the propagation of a …
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A study of a cyanobacterial bloom in the Albert Dock in Preston, Lancashire, UK
… However, the maintenance of crude cultures and axenic cultures acquired from culture collections was successfully achieved using Jaworski's Medium. MALDI TOF MS (Matrix-Assisted Laser Desorption I tonisation Time Of Flight Mass Spectrometry) was used to obtain characteristic mass spectral …
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The life history of Grimmia pulvinata (Hedw.) SM. and Tetraphis pellucida Hedw. in the laboratory and in the field
… leafy buds developed after prolonged growth of axenic protonemal cultures of G. pulvinata in daylight from the north sky but not in fluorescent light. Bud formation was not dependent on the inclusion of calcium or of EDTA in the medium. T. pellucida usually reproduces asexually by gemmae …
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Purification and properties of some fresh water and marine cyanobacteria belonging to the orders Chamaesiphonales and Pleurocapsales
… and Pieurocapsales were isolated in axenic culture from marine and fresh water sources. Two strains isolated from fresh water, both assignable to the genus Chamaesiphon, are unicellular cyanobacteria which reproduce by budding; the buds being formed in succession at one pole of the …
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Survival and chemical control of Cylindrocladium spp. inciting root rot of black walnut seedlings
… phenomenon exists in nature. Germinability of axenic, laboratory-grown microsclerotia of C. crotalariae, C. floridanum, and C. scoparium incubated 4 wk at 6 C ranged from 0 to 91.3% (mean = 37.7%) of the initial germinability. Partial recovery of laboratory-grown microsclerotia from the …
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Effect of herbicides on Cylindrocladium crotalariae and the cylindrocladium black rot (CBR) disease of peanut
The effects of herbicides on axenic growth of Cylindrocladium crotalariae and on the development of CBR in the greenhouse and in the field was studied. In laboratory tests, dinitramine at 1, 5, and 10 ug/ml increased axenic growth of two isolates of C. Crotalariae in herbicide-amended …
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Mikrobielle Ökologie N-Acyl-L-Homoserinlacton-produzierender Bakterien in der Rhizosphäre von Tomatenpflanzen
… plants cultivating systems were used: a defined axenic system and a complex soil system. The characterisation of the root colonisation behaviour was performed using confocal laserscanning microscopy (CLSM) and cell counting of bacteria. Fluorescence in situ hybridisation (FISH) and terminal …
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The growth promoting effect of 2,4-Dichlorophenoxyacetic acid (2,4-D) and 2,4,5-Trichlorophenoxyacetic acid (2,4,5-T) on Microcystis aeruginosa
… of this study showed that batch cultures of non-axenic Microcystis aeruginosa containing 2,4-D and 2,4,5-T exhibited significantly more growth than controls over a wide range of herbicide concentrations. Growth in 2,4-D treated cells was statistically significant from the control at a = 0.05 at …
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Dock180/Elmo proteins are critical regulators of phagocytosis,macropinocytosis and chemotaxis in Dictyostelium
… phototaxis. dock8""`l cells grow slowly in the axenic medium and are defective in chemotaxis, macropinocytosis and phagocytosis. dockA/8 double mutant cells show stronger defects in chemotaxis than either mutant by itself. In the dockN8""ll double mutant chemotaxis is almost completely inhibited …
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Environmental factor differences related to the growth of three species of Monoblepharella
Axenic cultures were made of three species of Monoblepharella. These were subsequently used in a physiological and nutritional investigation of the organisms. Growth of Monoblepharella laruei Springer (1945), Monoblepharella taylori Sparrow (1940) and Monoblepharella elongata Springer (1945) during …
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