Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 3 of 3 for “"AviTag"”.
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Elucidating the molecular mechanisms of p110δ activation in T cell antigen receptor signalling
… TCR signalling. This work has optimised the AviTag affinity purification system for the isolation of endogenous p110 isoforms from primary murine lymphocytes. A quantitative proteomics approach was subsequently used to identify endogenous proteins that interact with p110δ in primary activated …
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DISSECTING THE FUNCTION AND INTERACTOME OF PLEKHS1 IN PROSTATE TUMORIGENESIS
… could be specifically biotinylated in vivo (AviTag technology) and hence efficiently purified. Using this methodology, we have found that PLEKHS1 was specifically recovered with Class IA PI3Ks and that association was dramatically increased in Pten -/- prostate. To further investigate the …
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A dissection of class I phosphoinositide 3-kinase signalling in mouse embryonic fibroblasts and prostate organoids
… genetically-modified mice expressing endogenous, AviTagged class I PI3K subunits and used in experiments to define the subcellular localisation of class I PI3Ks. We found that following stimulation with PDGF, class IA PI3K subunits were unexpectedly depleted from the adherent basal membrane, in …