Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 62 for “"Autophosphorylation"”.
-
Autoregulatory and structural control of CaMKII substrate specificity
… with calcium signaling. Important sites of autophosphorylation, namely T287 and T306/7 (δ isoform numbering), reside within the ARD and control either CaM dependence or ability to bind to CaMKII respectively, thus determining various activation states of the kinase. Because …
-
Structure-function analysis of phototropin receptor kinases
… consequence of phototropin light stimulation is autophosphorylation. Phototropin autophosphorylation has previously been studied using a baculovirus/insect cell expression system, with the consequences of phototropin mutation on phototropin kinase activity within this heterologous system having …
-
Predicting and testing determinants of histidine-kinase functions by leveraging protein sequence information
… between separate signaling pathways, and how autophosphorylation occurs is central to kinase activity. In my thesis, I have taken biochemical and evolutionary approaches to identify molecular determinants for both dimerization specificity and autophosphorylation. To study dimerization …
-
Characterisation of CCaMK in symbiosis signalling
… to the visinin-like domain stimulates autophosphorylation, in particular at T271 (in Medicago truncatula CCaMK). However, the molecular mechanism by which this occurs was not well-established. By contrast, the mechanism of autophosphorylation of CaMKII, a similar animal protein, has …
-
Involvement of Platelet-Derived Growth Factor, PDGF Receptor Signaling, Focal Adhesion Kinase and SRC in Pressure-Induced Vascular Smooth Muscle Hypertrophy
… antibodies revealed that Src-pY<sup>418</sup> (autophosphorylation site) was elevated 3-fold over control values at 1 minute, whereas significant FAK-Y <sup>397</sup>(autophosphorylation site) activation was observed only following 3 minutes of pressure stimulus, and was blocked entirely by PP1, …
-
Protein phosphatases and protein kinases in dictyostelium
… least 0.03% of the total cellular protein. The autophosphorylation reaction can be inhibited by EDTA but not by EGTA. Following inhibition by EDTA, autophosphorylation can be reactivated by the addition of Mn²⁺, Mg²⁺, or Ca²⁺. Western blotting evidence is presented that shows cross reactivity of …
-
Analysis of the regulation mechanism of LmxMPK1, a Leishmania mexicana MAP kinase homologue
… results showed that it was a target for kinase autophosphorylation. The in vitro results also hinted that at least one other residue might also be subject to autophosphorylation, although to a lesser degree.;An inhibitor-sensitised system for studying LmxMPK1 was optimised, with the …
-
Defining the mechanisms of specificity in the symbiosis signalling pathway of Medicago truncatula
… A model for the activation of CCaMK via autophosphorylation has previously been proposed, but is unable to explain symbiosis signalling specificity. A mutational approach was therefore undertaken to determine the importance of calcium- and calmodulin-binding during the activation of …
-
An investigation of the activation of protein kinase complexes in the MyD88 signalling network
… protein kinases in some reports, but by autophosphorylation in others. In the first part of this thesis, I describe my work that has resolved this controversy by demonstrating that the activation of IKK induced by IL-1 (interleukin-1) or TNF (tumour necrosis factor) in embryonic …
-
Characterization of Hpk2-Rrp2, two-component regulatory system in Treponema denticola
… growth phase dependent manner. Tde1970 undergoes autophosphorylation and transfers phosphate to Tde1969. Henceforth the proteins encoded by these ORFs are designated as Hpk2 and Rrp2 respectively. Hpk2 autophosphorylation kinetics was influenced by environmental conditions and by the presence or …
-
The Functional Characterization of the Quorum Sensing E. Coli Regulators B and C in EHEC
… phosphate to the response regulator, QseB. The autophosphorylation of QseC is not affected by the addition of autoinducer-2 or intestinal hormones, including gastrin, galanin, and secretin. Additionally, autophosphorylation can be antagonized upon the addition of phentolamine, an a-adrenergic …
-
Cloning and characterisation of a Dictyostelium STE20-like protein kinase DST2
… and the regulatory domain (aa174) showed that autophosphorylation of DST2 occurson the regulatory domain and phosphorylates severin in the presence of a Mn2+ or Mg2+. Purified catalytic domain of PKA phosphorylated the regulatory domain of DST2 and caused an increase in the basal …
-
Crosstalk Between R1175 Methylation and Y1173 Phosphorylation Negatively Modulates Egfr-Mediated Erk Activation
… specifically enhances EGF-induced EGFR autophosphorylation at Y1173 residue. This novel modification crosstalk increases SHP1 recruitment to EGFR and suppresses EGFR-mediated ERK activation, resulting in inhibition of cell proliferation, migration, and invasion of EGFR-expressing cells. …
-
The Regulatory Role of Mixed Lineage Kinase 4 Beta in MAPK Signaling and Ovarian Cancer Cell Invasion
… activity. Moreover, MLK4beta; undergoes autophosphorylation and has kinase activity towards histone H2A, but has no kinase activity towards the MAP2K, MEK4/SEK1, a known substrate for MLK3 and other MAP3Ks. Furthermore, MLK4beta interacts with MLK3 and inhibits MLK3 activation. In …
-
Regulation of the oncogenic PIM1 kinase by SUMOylation
… that the K169R mutant is incapable of undergoing autophosphorylation similar to the K67M mutant of PIM1. This indicates that both K169 and K67 residues are critical for the intrinsic kinase activity, and may not represent SUMO-specific effects. On the other hand, autophosphorylation of E171A was …
-
Investigation of phototropin blue light receptor function and signalling in arabidopsis
… changes throughout the protein resulting in autophosphorylation of the N-terminal region by the kinase domain. Phototropins function redundantly to mediate a number of physiological responses in planta which serve to promote plant fitness and maximise photosynthetic potential. Phototropism, …
-
The effect of insulin-like growth factor-I (IGF-I), and an IGF-I-like factor secreted by human lung fibroblasts, on the growth of human lung carcinoma cells in vitro
… stimulated [1] a dose-dependent increase in the autophosphorylation of the type I IGF receptor, and [2] a dose-dependent increase (1.5- to 4-fold) in the phosphorylation of a tyrosine kinase-specific substrate. These results suggest that the growth promoting activity of IGF-I for the lung …
-
Serine/threonine phosphorylation in mycobacterium tuberculosis : identification of protein kinase B (PknB) substrates
… encoded by the ORF Rv1436, which demonstrates autophosphorylation activity and which phosphorylation is independent of PknB. Overall, the current study has contributed to advance our understanding of the signal transduction pathways and phosphoproteome in Mycobacterium tuberculosis.
-
Mechanism and specificity of bacterial two-component signaling systems
… and found that mutating them altered the rate of autophosphorylation. These analyses will allow rational rewiring of two component systems in vivo, and permit us to examine the fitness consequences of this altered specificity, providing insight into the evolutionary pressures on TCS systems.
Page 1 of 4