Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 18 of 18 for “"Autophagosome formation"”.
-
Regulation of autophagosome formation and maturation by neurodegeneration-associated proteins
… cargoes are captured into double-membraned autophagosomes, which form as outgrowths from the Ras-related protein 11A (RAB11A)-positive recycling endosomes. Autophagosomes are closed by the endosomal sorting complex required for transport (ESCRT) complex and then released by dynamin 2 (DNM2) …
-
Identification of regulators in autophagosome formation using image-based siRNA screening
… maturation, transportation and degradation of autophagosomes, where the double-membrane autophagosomes package the cargo for degradation. Therefore, understanding how autophagosomes form and are regulated is important in this field. Here we conducted a genome-wide siRNA screen using a …
-
Investigating the role of ULK1 kinase activity during autophagy
… whereby a double membrane organelle called an autophagosome, engulfs cytoplasmic components in order to degrade them via the lysosome. While autophagy is induced in response to various stresses to promote homeostasis and cell survival, a key mechanistic step in initiation of autophagosome …
-
Novel Insights into the Regulation of Autophagy in Saccharomyces Cerevisiae
… Npr3p function in the same complex and regulate autophagosome formation. During SL-induced autophagy, Iml1p can localize to the pre-autophagosomal structures, consistent with the role of the Iml1p complex in autophagosome formation. Moreover, a conserved domain in Iml1p was identified to be …
-
Interactions between Human Two-pore Channels and Nonaspanin Proteins
… TPCs knockdown, which indicates TM9SF1-induced autophagosome formation is dependent on TPCs knockdown. Therefore, it is of great interest to pursue to discover the detailed functional interaction between TM9SF1 and TPCs on cell autophagy, which is associated with a wide range of diseases …
-
A ROLE OF PRL-3 IN AUTOPHAGY AND ITS REGULATION IN HUMAN CANCERS
… ENHANCES HVPS34-BECLIN-1-DEPENDENT AUTOPHAGOSOME FORMATION, AND ACCELERATES ATG5 DEPENDENT LC3 CONVERSION. PRL-3 OVEREXPRESSION ALSO ACCELERATES THE DEGRADATION OF SQSTM/P62, WHICH IS A KNOWN AUTOPHAGY SUBSTRATE. SURPRISINGLY, PRL-3 ITSELF IS ALSO DEGRADED BY AUTOPHAGY, FORMING A …
-
Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System.
… cannabinoid (ACEA) and endocannabinoid (AEA) on autophagosome formation, and investigated the mechanisms involved. Our findings show that all three cannabinoids induce autophagy in a dose-dependent manner in fully differentiated CaCo2 cells. ACEA and AEA induced canonical autophagy, which was …
-
Role of ATG 5 in ER stress-induced apoptosis in meniscus.
… 5, a major autophagy related protein involved in autophagosome formation. Downregulation of ATG 5 protein levels was associated with decreased lipidation of LC3 and increased activation of Cleaved Caspase 3, which promoted meniscus cell apoptosis. Pretreatment of meniscus cells with 4-phenyl …
-
An assembly model for the autophagy initiation complex
… proteins, and pathogens. This is achieved by the formation of an autophagosome, a double membrane organelle that engulfs cytoplasmic components and then fuses with the vacuole or the lysosome leading to degradation of the engulfed components. Of note, defects in autophagy have been genetically …
-
Neuroprotective Effects of Sulforaphane and the Involvement of Autophagy
… (KO) mice. Atg16L1 is a protein necessary for autophagosome formation. Immunostainings assessed that autophagy was indeed fully supressed in KO cells. Preliminary results suggest that autophagy is involved in the neuroprotective effects of SFN. Conclusively, SFN was able to significantly …
-
Autophagy Delivers Viral Antigens for MHC Class II Presentation and is Regulated by Viral Infection
… human cells and demonstrated constitutive autophagosome formation in epithelial, B and dendritic cells. The autophagosome marker Atg8/LC3 strongly overlapped with markers of MHC class II loading compartments (MIICs) by confocal and immuno-electron microscopy, suggesting that autophagosomes …
-
Evading Innate and Adaptive Immunity During Adenovirus Cell Entry
… Although both Ad-WT and Ad-M1 infection induce autophagosome formation, a greater percentage of these autophagosomes contain Ad-M1 compared to Ad-WT. Further, significantly more Ad-M1 virus is found in lysosomes than Ad-WT several hours after infection. Inhibiting autophagy using RNAi silencing …
-
Visualization and quantification of autophagy in primary gastrointestinal epithelia cell cultures.
… and proteins to be degraded by the lysosome. The autophagosome is a double membrane vesicle which is used to encapsulate this cellular content. During the cellular response to starvation, autophagy can degrade cytoplasmic organelles and long-lived proteins in lysosomes, to recover amino acids …
-
PROGRAMMED DEATH LIGAND 1 (PD-L1) IMMUNE CHECKPOINT-MEDIATED STIMULATION OF MALIGNANT PHENOTYPES IN TUMOUR CELLS
… 3 (LC3)-II, and Beclin-1, as well as increased autophagosome formation. Using autophagy inhibitors, we showed that drug resistance induced by reverse PD-1/PD-L1 signaling is causally linked to increased autophagy. In addition, results of our studies demonstrated a role for extracellular …
-
TRPML1: Role In Autophagy And Potential Target To Treat Lysosomal Storage Disorders
… of PtdIns(3)P-binding proteins to the nascent autophagosome, whereas genetic or pharmacological inhibition of TRPML1 channel inhibits autophagy initiation. Importantly, alteration of this function has pathological consequences, and thus we found that autophagosome formation is impaired in human …
-
The Recycling GTPase, RAB-10, Regulates Autophagy Flux in <i>Caenorhabditis elegans</i>
… is a dynamic and catabolic process involving the formation of a double-membrane vesicle called the autophagosome, which engulfs long-lived proteins and damaged organelles. Endocytosis involves the uptake of extracellular material into the cell through the formation of intracellular vesicles termed …
-
Molecular Determinant of Mitochondrial Shape Change
… mutants. Miro1-dependent MiST is essential for autophagosome formation that is attenuated in cells harboring Miro1 EF1 mutants. Remarkably, loss of cCa2+ sensitization by Miro1 prevented MiST and mitigated autophagy. These results demonstrate that an interplay of ions and metabolites function in …
-
Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease
… as dysfunction in any of the stages of autophagosome formation could lead to neurodegeneration. In Chapter VI we examine the ability of the antihistamine drug Latrepirdine to induce autophagy in zebrafish larvae. The findings of this chapter indicate Latrepirdine is capable of inducing …