Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"Autophagosome"”.
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Cationic amphiphilic drug-induced autophagosome accumulation is due to autophagosome sequestration within vimentin intermediate filament networks resulting in prolonged autophagosome half-life
<p>Accumulations of autophagosomes and non-esterified cholesterol are</p> <p>observed in several cell lines derived from lysosomal storage diseases,</p> <p>including Niemann Pick Type C (NPC). The relationship between</p> <p>autophagosome accumulation and lysosomal non-esterified cholesterol is</p> …
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Characterising Futile Autophagosome Based Toxicity and its Implications in Disease
… cytoplasmic material into vesicles called autophagosomes, which then shuttle to lysosomes for degradation. Autophagy is implicated in numerous diseases, largely in a pro-survival role. However, autophagy has also been suggested as a form of programmed cell death (PCD), from cases of dying …
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Regulation of autophagosome formation and maturation by neurodegeneration-associated proteins
… cargoes are captured into double-membraned autophagosomes, which form as outgrowths from the Ras-related protein 11A (RAB11A)-positive recycling endosomes. Autophagosomes are closed by the endosomal sorting complex required for transport (ESCRT) complex and then released by dynamin 2 (DNM2) …
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Identification of regulators in autophagosome formation using image-based siRNA screening
… maturation, transportation and degradation of autophagosomes, where the double-membrane autophagosomes package the cargo for degradation. Therefore, understanding how autophagosomes form and are regulated is important in this field. Here we conducted a genome-wide siRNA screen using a …
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Investigating the role of ULK1 kinase activity during autophagy
… whereby a double membrane organelle called an autophagosome, engulfs cytoplasmic components in order to degrade them via the lysosome. While autophagy is induced in response to various stresses to promote homeostasis and cell survival, a key mechanistic step in initiation of autophagosome …
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An assembly model for the autophagy initiation complex
… This is achieved by the formation of an autophagosome, a double membrane organelle that engulfs cytoplasmic components and then fuses with the vacuole or the lysosome leading to degradation of the engulfed components. Of note, defects in autophagy have been genetically linked with cancer, …
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Visualization and quantification of autophagy in primary gastrointestinal epithelia cell cultures.
… and proteins to be degraded by the lysosome. The autophagosome is a double membrane vesicle which is used to encapsulate this cellular content. During the cellular response to starvation, autophagy can degrade cytoplasmic organelles and long-lived proteins in lysosomes, to recover amino acids …
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Studies on the autophagy gene WIPI4 and its interactor UBR5
… WIPI4 as a regulator of the closure of autophagosomes. Although many proteins have been found to indirectly regulate autophagosome closure, the exact mechanism of this process has remained unclear. This thesis explored the regulatory mechanism of GABARAP by WIPI4. GABARAP is a potential …
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INVESTIGATING THE ROLE OF SNAP29 AND THE AUTOPHAGY PATHWAY IN DROSOPHILA MELANOGASTER MODELS OF C9ORF72 - LINKED ALS/FTD.
… G4C2 toxicity did not significantly disrupt autophagosome maturation or later stages of autophagy, despite its high toxicity levels in vivo. These findings suggest that G4C2 toxicity alone may not severely impair the autophagy pathway and, in turn, that disruption in degradative autophagy may …
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TRPML1: Role In Autophagy And Potential Target To Treat Lysosomal Storage Disorders
… of PtdIns(3)P-binding proteins to the nascent autophagosome, whereas genetic or pharmacological inhibition of TRPML1 channel inhibits autophagy initiation. Importantly, alteration of this function has pathological consequences, and thus we found that autophagosome formation is impaired in human …
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The Recycling GTPase, RAB-10, Regulates Autophagy Flux in <i>Caenorhabditis elegans</i>
… of a double-membrane vesicle called the autophagosome, which engulfs long-lived proteins and damaged organelles. Endocytosis involves the uptake of extracellular material into the cell through the formation of intracellular vesicles termed endosomes. Although both endocytosis and …
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Novel Insights into the Regulation of Autophagy in Saccharomyces Cerevisiae
… Npr3p function in the same complex and regulate autophagosome formation. During SL-induced autophagy, Iml1p can localize to the pre-autophagosomal structures, consistent with the role of the Iml1p complex in autophagosome formation. Moreover, a conserved domain in Iml1p was identified to be …
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The Role of Autophagy in Early Development and Tumor Suppression Using a Zebrafish Model System
… into a double membraned structure called the autophagosome. By using genetically manipulated autophagy-deficient models, important roles for autophagy in development and tumorigenesis have been suggested. Genetic analyses indicate that autophagy is essential for eukaryotic differentiation and …
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Biochemical and Genetic Studies of Beclin 1 Function in Autophagy
… Beclin 1 and Atg14L synergistically promote autophagosome biogenesis while over-expression of Rubicon causes formation of aberrant late endosomes/early lysosomes and blocks autophagosome maturation. Therefore Atg14L and Rubicon regulate autophagy in opposite directions and at different steps, …
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Autophagy Delivers Viral Antigens for MHC Class II Presentation and is Regulated by Viral Infection
… human cells and demonstrated constitutive autophagosome formation in epithelial, B and dendritic cells. The autophagosome marker Atg8/LC3 strongly overlapped with markers of MHC class II loading compartments (MIICs) by confocal and immuno-electron microscopy, suggesting that autophagosomes …
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Interactions between Human Two-pore Channels and Nonaspanin Proteins
… TPCs knockdown, which indicates TM9SF1-induced autophagosome formation is dependent on TPCs knockdown. Therefore, it is of great interest to pursue to discover the detailed functional interaction between TM9SF1 and TPCs on cell autophagy, which is associated with a wide range of diseases …
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A ROLE OF PRL-3 IN AUTOPHAGY AND ITS REGULATION IN HUMAN CANCERS
… ENHANCES HVPS34-BECLIN-1-DEPENDENT AUTOPHAGOSOME FORMATION, AND ACCELERATES ATG5 DEPENDENT LC3 CONVERSION. PRL-3 OVEREXPRESSION ALSO ACCELERATES THE DEGRADATION OF SQSTM/P62, WHICH IS A KNOWN AUTOPHAGY SUBSTRATE. SURPRISINGLY, PRL-3 ITSELF IS ALSO DEGRADED BY AUTOPHAGY, FORMING A …
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Atg11 and Atg9 interaction in selective autophagy
… infections. It involves the formation of an autophagosome, a double-membrane vesicle that takes the cargo to the vacuole/lysosome for degradation. Atg11, the central organizer of selective autophagy, acts as a scaffold protein, interacting with Atg9, Atg1 and other Atg proteins. Our research …
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Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System.
… cannabinoid (ACEA) and endocannabinoid (AEA) on autophagosome formation, and investigated the mechanisms involved. Our findings show that all three cannabinoids induce autophagy in a dose-dependent manner in fully differentiated CaCo2 cells. ACEA and AEA induced canonical autophagy, which was …
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Synaptojanin1 is involved in endolysosomal trafficking in cone photoreceptors
… photoreceptor development. The accumulation of autophagosomes in the nrca14 mutant is due, at least in part, to impaired autophagosome maturation that is not caused by a decrease in autophagosome mobility. The causative mutation in the nrca14mutant is in the gene encoding a polyphosphoinositide …
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