Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Atoh1"”.
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Promotion of Hair Cell Fate by Atoh1 and MicroRNA-183 Family
… good candidates for regenerative studies. Atoh1 expression is widely accepted to be necessary and contextually sufficient for driving hair cell fate. Furthermore, the miRNA-183 family is known to be expressed at the time of hair cell differentiation, and mutations in miR-96 (one member of …
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Role of the ubiquitin-proteasome pathway in the inner ear : identification of an E3 ubiquitin ligase for Atoh1
Atoh1, the proneural basic-helix-loop-helix transcription factor, is critical for the differentiation of inner ear hair cells. Hair cells do not develop in mice that lack Atoh1, and overexpression of the transcription factor in embryonic ears induces differentiation of extra hair cells. The level …
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Temporal and spatial controls of cell fate specification in the cerebellum
… by the expression of the transcription factor Atoh1, which gives rise to different cell types contributing to both extra-cerebellar and cerebellar populations. In chick embryos, I have investigated the timing, morphology and gene expression of successive cohorts of rhombic lip derivatives using …
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The origin and properties of pro-oncogenic fields in the intestinal epithelium
… in vivo. Cre mediated lineage tracing of Atoh1 expressing early secretory progenitors carrying KrasG12D mutations demonstrated that KrasG12D expression does not change cell fate choices in homeostasis but appears to increase Atoh1+ stem cell contribution in the small intestine after Lgr5 …
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Characterization of the Key Mouse Cochlear Developmental Genes for Auditory Hair Cell Regeneration
… SCs into HCs, through ectopically expressing Atoh1 in SCs. Taken together, Atoh1 is able to reprogram SCs into HCs, and inactivation of p27 or Sox2 can achieve proliferation of SCs. This work builds the foundation for future HC regeneration studies. Combining these two manipulations may …
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Derivation of next generation research models of Parkinson’s disease
… a significant finding: ectopic expression of ATOH1 alone is sufficient to generate Tyrosine hydroxylase (TH)-positive neurons. Additionally, we identified several TF combinations that also induced TH-positive neurons. Single-cell RNA sequencing analysis of neuronal culture generated through …
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Conditional Loss of Engrailed 1/2 in Rhombic Lip-Derived Neurons Increases Intrinsic Rhythmicity and Decreases Overall Variability of Eupneic Respiration
… an elegant model of cerebellar neuropathology, Atoh1-En1/2 CKO, where mutant animals have conditional loss of the developmental patterning gene Engrailed 1/2 in rhombic lip-lineage neurons and exhibit a proportional scaling-down of neuron number in hypoplastic lobules of the CRB. We utilized …
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Defined populations of inner ear progenitor cells show limited and distinct capacities for differentiation into hair cells, neurons, and glia
… Whole-cell patch clamp recordings showed that Atoh1-nGFP+ cells derived from both cochlear and vestibular tissue had voltage gated ion channels that were typical of postnatal hair cells. Only vestibular-derived AtohinGFP+ cells, however, had Ih, a hyperpolarization-activated current typical of …
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Combination of Compounds A2CE and C18 for Cochlear Hair Cell Regeneration in Adult Mice
… non-canonical role in suppressing a cofactor for Atoh1, which is an upstream of POU4F3 and is an important developmental gene in hair cell development. We gave transtympanic injections of our drug cocktails to either FVB or Sox2 CreER; Tdtomato mice and collected their cochlea 4 weeks following …