Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Atg8"”.
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ATG8 Regulation and Function in Non-Canonical Autophagy
… degradation. In a defining step of this pathway, ATG8 subfamily proteins (LC3s and GABARAPs) are recruited to double membrane autophagosomes where they facilitate substrate capture, membrane biogenesis and maturation. LC3 proteins can also be recruited to single membrane, endolysosomal …
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Biochemical and Structural Characterization of the Atg8/LC3 Lipidation Pathway
Atg8 and its eukaryotic orthologues LC3 and GARBARAP family proteins (referred here to Atg8 family proteins) play crucial roles in autophagy through their covalent ligation to lipids, typically phosphatidylethanolamine (PE), in a process known as lipidation. Lipidation of Atg8 family proteins …
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Autophagy and EVs in cancer cells. Effect of ATG8 knockdown on the biogenesis of CD81+ extracellular vesicles.
… or without autophagy disruption and determine if ATG8 gene knockout, or knockout of its sub-families LC3 and GABARAP, impacts the number and size of EVs. The sub-objective was to positively identify EVs as the isolated particles by examining protein markers specific to EVs and imaging the vesicles …
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Regulators of autophagy in Leishmania major
… cycle progression and virulence of Leishmania. ATG8 is a ubiquitin like protein that is required for the formation of autophagosomes, and Leishmania uniquely possesses a set of ATG8-like proteins in addition to ATG8, that are distributed in three multi-gene families called ATG8A, ATG8B and …
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The role of CASM in the endolysosomal damage response
… and establishes the role of Conjugation of ATG8 to Single Membranes (CASM) in the lysosomal damage response. Findings conclusively showed that following lysosomal damage, CASM was rapidly and potently activated, and subsequently mediated the lipidation of ATG8 (e.g. LC3A) family proteins …
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Novel Insights into Ubiquitin-like Protein E1-E2 Interactions
… (Sumo utilizes a canonical E1 and E2) and the Atg8 pathway (Atg8 is a UBL involved in autophagy that utilizes a non-canonical E1‑E2 pair).</p> <p>Sumo conjugation is initiated by the heterodimeric Aos1‑Uba2 E1 enzyme (in humans called Sae1‑Uba2), which activates Sumo's C-terminus, binds the …
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Estudio funcional de la autofagia por estrés específico en el cloroplasto de la microalga modelo Chlamydomonas reinhardtii
… de edición genético CRISPR/Cas9. El mutante atg8 presenta una inserción en el gen ATG8 que codifica una proteína clave en el mecanismo central de la autofagia. Nuestros resultados indican que esta estirpe mutante presenta un retraso en su crecimiento al entrar en fase estacionaria e …
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An investigation of the interaction between the immune system GTPase GIMAP6 and an autophagy gene 8 homologue GABARAPL2
… homologue of the autophagy-related protein 8 (Atg8). Using bacterially expressed GABARAPL2 and GIMA- P6 I have shown that the interaction between the two proteins is direct. My studies have attempted to gain an understanding of the molecular requirements for this interaction using site-directed …
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Molecular analysis of vesicle biogenesis during autophagy
… candidate to follow the membrane flow is Atg8 that is covalently coupled to membranes via phosphatidylethanolamine (PE). This study describes for the first time a peripheral pool of Atg8 located at endosomes. Its formation is dependent on Atg1 and Atg27 as described for the Atg9 peripheral …
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Visualization and quantification of autophagy in primary gastrointestinal epithelia cell cultures.
… cultured from human intestinal biopsy material. Atg8/LC3 is the major structural protein of the autophagosome. Images of GFP-Atg8/LC3 puncta generated in response to starvation, or NOD2 signalling, were taken by fluorescence microscopy, and pixel densities were rendered to generate digital …
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Identification of components required for mitophagy and mitochondrial inheritance by genetic screens in yeast
… with the autophagic membrane expansion factor Atg8, which suggests that ERMES plays a role during growth of this membrane. Consequently, ERMES mutants show aberrant autophagic membrane structures, which can again be rescued by artificial mitochondria-ER tethering. It can thus be hypothesized …
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Cardiolipin Regulates Mitophagy Through The Pkc Pathway
… lethal/sick with the general autophagy mutants atg8Δ, atg18Δ and mitophagy mutant atg32Δ, suggesting that autophagy/mitophagy may be deficient in cells lacking CL. Microscopic examination of mitophagy revealed decreased translocation of GFP-tagged mitochondrial proteins into the vacuole of crd1Δ …
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Modelling neurodegenerative diseases in human iPSC-derived neurons
… is sufficient to reduce transcription of the ATG8 gene family, which are core components of macroautophagy. This work exemplified both the strengths and weaknesses of iPSC-derived neurons; namely, they are tractable and able to recapitulate neural phenotypes, but deriving new model lines …
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MIT/TFE FACTORS CONTROL ER-PHAGY VIA TRANSCRIPTIONAL REGULATION OF FAM134B
… as CALCOCO1 and SQSTM1), that bind to cytosolic Atg8-family proteins via the LC3-Interacting Region (LIR) mediating the delivery of specific ER subdomains to lysosomes for degradation. Although the role of the ER-phagy in the regulation of ER size and cellular proteostasis has been well …
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Autophagy Delivers Viral Antigens for MHC Class II Presentation and is Regulated by Viral Infection
… B and dendritic cells. The autophagosome marker Atg8/LC3 strongly overlapped with markers of MHC class II loading compartments (MIICs) by confocal and immuno-electron microscopy, suggesting that autophagosomes frequently fuse with MIICs. Furthermore, this pathway was of functional relevance, …