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Showing 1 to 20 of 123 for “"Asparagine"”.
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Investigating the pathway of asparagine-linked glycoprotein biosynthesis
The biosynthesis of asparagine-linked glycoproteins, highly conserved throughout all eukaryotes, requires a dolichylpyrophosphate-linked tetradecasaccharide precursor (Dol-PP-GlcNAc2Man9Glc3), from which the tetradecasaccharide is transferred co-translationally to nascent polypeptides in the lumen …
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Investigating asparagine-linked protein glycosylation in eukaryotic and prokaryotic systems
… the formation of a -glycosylamine linkage to an asparagine residue within the Asn-Xaa-Ser/Thr consensus sequence. This modification is found in organisms from eukaryotic, archaeal and bacterial domains and is implicated in numerous cellular processes. Recently, a system of N-linked glycosylation …
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Investigation of asparagine-linked glycosylation in archaeal and bacterial systems
Asparagine-linked protein glycosylation entails the stepwise assembly of an oligosaccharide onto a polyisoprenyl diphosphate carrier, followed by the en bloc transfer of the glycan onto acceptor proteins by oligosaccharyl transferase (OTase). It is now clear that this N-linked protein modification …
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Characterization of Enzymes in the L-Asparagine Metabolism Pathway in Legumes
… understanding of nitrogen metabolism. L-asparagine (Asn) is an important source of nitrogen stored and transported in higher plants, favoured in legumes due to its advantageous 2N:4C ratio. The catabolism of Asn in tissues occurs through two major pathways: deamidation and transamination. …
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Studies on the Asparagine-Linked Oligosaccharides From Cartilage-Specific Proteoglycan (Chondrocytes)
… keratan sulfate, O-linked oligosaccharides, and asparagine-linked oligosaccharides. The asparagine-linked oligosaccharides found on PG-H were investigated in these studies.
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Investigating asparagine-linked glycosylation substrate : specificity and effects on protein folding
… oligosaccharide is covalently attached the asparagine side chain of an acceptor protein. This process involves numerous enzymes, produces a diverse set of oligosaccharide structures, and results in a variety of structural and functional effects on the glycoprotein. Research discussed in this …
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The Essential Role of the Non-Essential Amino Acid Asparagine in Lymphoid Malignancies
… and metabolism therapeutically. Currently, asparagine depletion via L-Asparaginase in acute lymphoblastic leukemia (ALL) remains the only clinically approved therapy to date. In the first project, we showed that ALL cells are auxotrophic for asparagine and rely on exogenous sources for this …
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Effect of nitrogen form and genetic variations of the Asparagine-cycling pathway on maize ear growth
… dry weight as well as the transport amino acids asparagine and glutamine. This nitrogen-induction experiment was duplicated, and root, shoot, and ear tissues were profiled via RNA-Seq to characterize responses to ammonia or nitrate at four or eight hours after nitrogen treatment. Results suggest …
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Nitrogen and sulfur effects on hard winter wheat quality and asparagine concentration and survey of Kansas soil sulfur conditions
… deficiency can also lead to an increase of free asparagine concentration which indicates acrylamide forming potential of baked products. Acrylamide is a potential carcinogen; S fertilization has been shown to decrease acrylamide forming potential in wheat flour. Therefore, the objectives of this …
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Transcriptional Regulation of the Metabolic Response to Therapy in Leukemia
… as a critical regulator of the response to asparagine deprivation in acute lymphoblastic leukemia (ALL). We found that under asparagine depleted conditions ZBTB1 enables cellular proliferation by promoting the synthesis of asparagine from aspartate. Mechanistically, ZBTB1 binds directly to a …
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Behavioral Adaptation in Bacillus Subtilis: The Role of Selective Methylation Changes and Heterologous Receptors in Mcpb-Mediated Signaling
… of higher-order receptor complexes. Therefore, asparagine chemotaxis likely depends on the presence of heterologous receptors. To explore these processes, a study was performed on strains expressing mutant McpB proteins (with aspartates substituted at the sites of methylation) in genetic …
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Investigating the Therapeutic Potential of L-asparaginase in Myeloid Leukemia
… is an enzyme that degrades extracellular asparagine and glutamine. Interestingly, a growing body of evidence has demonstrated cancer metabolism is a distinctive trait that can be targeted by reducing nutrient availability. Therefore it is plausible that cancer metabolism will be affected …
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The Role of Nutrient Availability in Therapeutic Response of Leukemia
… (ASNase), a therapy that depletes plasma asparagine and targets leukemic cells with insufficient asparagine synthesis. Our approach revealed thiamine pyrophosphate kinase 1 (TPK1), which converts vitamin B1 (thiamine) into the cofactor thiamine pyrophosphate (TPP), as a metabolic …
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ΜΕΛΕΤΗ ΤΗΣ L-ΑΣΠΑΡΑΓΙΝΑΣΗΣ ΣΤΟ ΠΡΩΤΟΖΩΟ T. PYRIFORMIS
… THE PELLICLES. INTACT PROTOZOA CAN HYDROLYZE L- ASPARAGINE AT A RATIO OF 60 NMOLE/10 6 CELLS/HR WHILE THE INFLUX OF THIS AMINOACID IS ONLY 8 NMOLE/10 6 CELLS/HR. UPON THESE STUDIES L- ASPARAGINASE OF PELLICLES CAN BE CONSIDERED AS AN ECTOENZYME. L- ASPARAGINASE CAN BE SOLUBILIZED BY CHAOTROPIC …
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Development of transition state analogues targeting chitinases and oligosaccharyl transferase
… (OT) plays a central role in the biosynthesis of asparagine-linked glycoproteins in eukaryotic systems. The glycosylation step catalyzed by OT involves the co-translational transfer of a tetradecasaccharide from a dolichyl-pyrophosphate carrier to an asparagine side-chain within the …
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The asymmetric synthesis of (2S,3R)-capreomycidine and the total synthesis of capreomycin IB
… has been greatly reduced by the utilization of asparagine in the peptide preparations as a masked precursor to diaminopropanoic acid. Conversion of the asparagine residue to the diaminopropanoic residue is accomplished by a Hofmann rearrangement.
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IMPROVING GLYCOSYLATION EFFICIENCY IN ESCHERICHIA COLI BY MAMMALIAN-CELL-INSPIRED PROTEIN-PROTEIN INTERACTIONS
Asparagine linked glycosylation (N-glycosylation) is one of the most common post-translational modifications found in proteins. The central enzyme in the N-glycosylation pathway responsible for the transfer of glycans onto asparagine residues is called the oligosaccharyltransferase (OST). In …
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Biochemical and biophysical investigations of N-linked glycosylation pathways in archaea
Asparagine-linked glycosylation is an abundant and complex protein modification conserved among all three domains of life. Much is known about N-glycan assembly in eukaryotes and selected bacteria, in which the oligosaccharyltransferase (OTase) carries out the en bloc transfer of glycans from …
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Characterising mechanisms of regulation of hypoxia-inducible factors.
… abrogation of hydroxylation of a conserved asparagine residue. Hypoxia and chemical mimetics of hypoxia, such as iron chelators and analogs of 2-oxoglutarate, prevented hydroxylation of the asparagine allowing the CAD to interact with the transcriptional coactivator protein p300. We then …
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Structural specificity in coiled coils : a and d position polar residues
… more sensitive to polar residues. Two residues, asparagine and glutamine, were found to be much more destabilizing when placed at d positions than any residues at the a positions. In addition to the known ability of a single asparagine at the a position to specify coiled-coil dimers, it was found …
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