Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Asparaginase"”.
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Investigating the Therapeutic Potential of L-asparaginase in Myeloid Leukemia
… existing drugs or identifying new targets. L-asparaginase, a component of standard acute lymphocytic leukemia (ALL) treatment, is an enzyme that degrades extracellular asparagine and glutamine. Interestingly, a growing body of evidence has demonstrated cancer metabolism is a distinctive trait …
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Adverse Effects of Asparaginase in Pediatric Patients with Acute Lymphoblastic Leukemia
… is the most common type of childhood cancer. Asparaginase is a critical treatment component for ALL. However, its use is complicated by adverse effects, such as hypersensitivity, osteonecrosis and pancreatitis.</p> <p>Hypersensitivity to asparaginase typically requires discontinuation of …
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The effect of degradation on the efficacy and utility of the biopharmaceutical enzyme Erwinia Chrysanthemi L-Asparaginase
… case. This problem is relevant to the enzyme L-asparaginase from the plant pathogen Erwinia chrysanthemi (ErA, or Erwinase), which is used as a treatment for acute lymphoblastic leukaemia (ALL). This research programme has addressed this issue by examining the effect of potentially adverse …
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ΜΕΛΕΤΗ ΤΗΣ L-ΑΣΠΑΡΑΓΙΝΑΣΗΣ ΣΤΟ ΠΡΩΤΟΖΩΟ T. PYRIFORMIS
L-ASPARAGINASE OF TETRAHYMENA PYRIFORMIS IS A MEMBRANE BOUND ENZYME. MOST OF ITS ACTIVITY ( 80%) IS ASSOCIATED WITH MICROSOMES AND THE REMAINING WITH THE PELLICLES. INTACT PROTOZOA CAN HYDROLYZE L- ASPARAGINE AT A RATIO OF 60 NMOLE/10 6 CELLS/HR WHILE THE INFLUX OF THIS AMINOACID IS ONLY 8 NMOLE/10 …
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Process integration of cell disruption and fluidised bed adsorption of microbial enzymes: application to the retro-design of the purification of L-asparaginase
… Pilot-scale purification of the enzyme L-asparaginase from unclarified Erwinia chrysanthemi disruptates exploiting this novel approach yielded an interim product which rivalled or bettered that produced by the current commercial process employing discrete operations of alkaline lysis, …
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The Essential Role of the Non-Essential Amino Acid Asparagine in Lymphoid Malignancies
… Currently, asparagine depletion via L-Asparaginase in acute lymphoblastic leukemia (ALL) remains the only clinically approved therapy to date. In the first project, we showed that ALL cells are auxotrophic for asparagine and rely on exogenous sources for this non-essential amino acid. …
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Discovery and characterization of genes controlling nitrogen utilization in maize
… of autophagy and nutrient remobilization, and L-asparaginase that mediates nutrient sensing and rate of N flux The functional effects of ZmHVA22 and L-asparaginase on N utilization were investigated in both inbred and hybrid genotypes with different genetic variants for these two genes. Field …
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Genome editing with CRISPR-Cas9 in the Illinois long term selection experiment
… A second CRISPR experiment targeted the L-Asparaginase (ASNase) gene, which exhibits reduced gene expression in IHP1 compared to ILP1. The goal was to test whether reducing ASNase gene function can increase grain protein concentration in the ILP1 background. Four guide RNAs were designed …
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Effect of nitrogen form and genetic variations of the Asparagine-cycling pathway on maize ear growth
… genes, Asparagine synthetase (AS) and Asparaginase (ASNase). We examined the effect of these two genes on ear growth using a range of populations that contain variants of these genes. Greater AS expression was associated with improved ear growth, whereas very low ASNase activity was …
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Modulating Tumor Immune Microenvironment Using Heterologous Proteins
… for the systemic and selective depletion (no asparaginase activity) of circulating glutamine in live animals. In the second part of the thesis, we have engineered T cells to increase their adhesion to endothelial cells with the overall objective of increasing their tumor infiltration. We …
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Modulating Tumor Immune Microenvironment Using Heterologous Proteins
… for the systemic and selective depletion (no asparaginase activity) of circulating glutamine in live animals. In the second part of the thesis, we have engineered T cells to increase their adhesion to endothelial cells with the overall objective of increasing their tumor infiltration. We …
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The Role of Nutrient Availability in Therapeutic Response of Leukemia
… metabolic modifiers of the response to L-asparaginase (ASNase), a therapy that depletes plasma asparagine and targets leukemic cells with insufficient asparagine synthesis. Our approach revealed thiamine pyrophosphate kinase 1 (TPK1), which converts vitamin B1 (thiamine) into the cofactor …
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Characterization of novel virulence factors of Shigella flexneri and development of Caenorhabditis elegans as an animal model for Shigella infection
… of this study is to investigate the role of L-asparaginase (AnsB) and gamma-glutamyltranspeptidase (GGT) in S. flexneri pathogenesis. Using a reverse genetic approach I found that AnsB and GGT are required for bacterial adherence to host cells. In vivo studies in both the Caenorhabditis elegans …
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Characterization of novel virulence factors of Shigella flexneri and development of Caenorhabditis elegans as an animal model for Shigella infection
… of this study is to investigate the role of L-asparaginase (AnsB) and gamma-glutamyltranspeptidase (GGT) in S. flexneri pathogenesis. Using a reverse genetic approach I found that AnsB and GGT are required for bacterial adherence to host cells. In vivo studies in both the Caenorhabditis elegans …
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Transcriptional Regulation of the Metabolic Response to Therapy in Leukemia
… vitro but are also sensitive to treatment with L-asparaginase, a chemotherapy that reduces serum asparagine, in in vivo xenograft models of ALL. Additionally, this work clarifies the metabolic stress induced by CPI-613, a lipoic acid analog designed to inhibit the function of Pyruvate …
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Discovery of novel regulators and genes in nitrogen utilization pathways in maize
… when ILP1 alleles for asparagine synthetase 3 or asparaginase were introgressed. Protein concentration in the ILP1 background increased commensurately, by 0.8% on average in the near isogenic lines. This change in grain protein was significant, but was still insufficient to account for all of the …
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The Mechanism of Activation of Potassium Dependent and Potassium Independent Asparaginases from Common Bean (Phaseolus vulgaris)
L-asparaginases play an important role in nitrogen mobilization in plants. This study investigated the biochemical and biophysical properties of potassium dependent (PvAspG1) and potassium independent (PvAspG-T2) L-asparaginases from P. vulgaris. Previous studies revealed that PvAspG1 requires …