Global ETD Search
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Showing 1 to 8 of 8 for “"Artificial Enzymes"”.
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Novel imprinted polymers as artificial enzymes
Derivatives of L-histidine were investigated as suitable models for the Asp-His couple found in the catalytic triad of serine proteases. A combination of molecular dynamics and IH NMR spectroscopy suggested that the most populous conformations of N-acetyl-L-histidine and the N-acetyl-L-histidine …
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The synthesis of stable nanotags for the detection of biomolecules using surface enhanced Raman scattering
Artificial enzymes have become an increasingly interesting area of research due to their many advantages over natural protein enzymes which are expensive, difficult to isolate and unable to stand harsh environments. An important area of this research involves using metal nanoparticles as artificial …
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Molecularly imprinted nanoparticles to mimic natural enzymes for substrate-selective catalysis
Enzymes are extraordinary catalysts with remarkable catalytic efficiency and selectivity. One of the biggest challenges in mimicking natural enzymes is constructing a substrate-tailored active site with accurately positioned catalytic groups. The molecular imprinting technique in doubly …
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Development of a synthetic enzyme for detection and quantitation of Thiols
… $\rm M\sp{-1}s\sp{-1}$ at pH 7.0. Synthesis of artificial enzymes by coupling these catalysts with the primary or secondary monotosylate of $\beta$-cyclodextrin yielded unique species based on RP-HPLC, and UV-Visible spectroscopy. After isolation by RP-HPLC, synzymes were examined for thiol …
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Characterization and evolution of artificial RNA ligases
Enzymes enable biocatalysis with minimal by-products, high regio- and enantioselectivity, and can operate under mild conditions. These properties facilitate numerous applications of enzymes in both industry and research. Great progress has been made in protein engineering to modify properties such …
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PNA-Ligand Bioconjugates as Potential Building Blocks for Sequence-Specific, Metal-Mediated DNA-/RNA-Cleavage
… bioconjugates is described. The design of artificial enzymes for sequence-specific DNA and RNA cleavage is one of the most challenging problems in modern biotechnology, since the commercially available biotools are restricted to a limited number of promoter sequences. Possible nuclease …
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Gene editing in Aedes aegypti
… segments from the Ae. aegypti genome. All four enzymes were found to be active in Ae. aegypti; however, the activity of Y2-I-AniI was higher compared to the other three enzymes. Single-strand annealing (SSA) and non-homologous end-joining (NHEJ) pathways were identified as mechanisms to repair …
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Defining the substrate scope of DNAzyme catalysis for reductive amination with aliphatic amines
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-05-01