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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Arp2/3 complex"”.
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Mechanisms Of Activation And Inhibition Of Arp2/3 Complex
Arp2/3 complex nucleates branched actin networks that drive cell motility. It consists of seven proteins, including actin-related proteins (Arps) 2 and 3. In isolation Arp2/3 complex is in an inactive, splayed conformation. Two nucleation-promoting factors (NPFs) bind Arp2/3 complex during …
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Biochemical Reconstitution and Functional Characterization of the WAVE Regulatory Complex
… to stimulate the actin-nucleating activity of Arp2/3 complex in response to upstream signals from the Rho family GTPases Cdc42 and Rac1. While WASP regulation by GTPases and kinases is well characterized both biochemically and structurally, little is known about WAVE regulation. WAVE exists as …
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The characterization of collapsin response mediator protein-1 (CRMP-1) for Arp2/3 dependent actin structures
… actin assembly is the actin nucleator, the Arp2/3 complex. Upon the hierarchical regulation of the activity of the Arp2/3 complex, the cell can build the correct actin framework spatially and temporally in response to different cellular signals. Understanding the molecular mechanism of how …
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Effector-driven actin reorganisation restricts Salmonella invasion.
… activate host Rho GTPases and subsequently the Arp2/3 complex, which results in actin reorganisation. Whereas the role of SopE in promoting bacterial uptake is well-established, the precise function of SopE2 is not known. SopE2 was previously shown to activate Cdc42, which leads to the …
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Self-assembly of filopodia-like structures on supported lipid bilayers
… but instead by the self-organization of tip complex assembly on uniform PI(4,5)P 2-enriched regions. BAR domain protein toca-1 recruits N-WASP then the Arp2/3 complex and actin assembly follow. Elongation proteins Diaphanous-related formin, VASP and fascin are recruited later. The Arp2/3 …
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Regulation of Myo5p function by phosphorylation
… to induce actin polymerization through the Arp2/3 complex. Within this work, Myo5p serine 1205, located N-terminal to the sequence that interacts with and activates the Arp2/3 complex, was identified as a phosphorylation site for casein kinase II (CKII). This phosphorylation event appears to …
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The S. cerevisiae calponin homologue SCP1 regulates stability and organization of the actin cytoskeleton
… modulates Abpl-dependent activation of the Arp2/3 complex. In summary, Scpl may function in complex with Abpl to regulate actin nucleation by the Arp2/3 complex.
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Regulation of WASP in Actin Signaling: From Angstroms to Microns
… By determining the solution structure of a complex between the WASP GBD and 1R, I showed that EspFU binds to WASP by mimicking the C region of the VCA domain. 1R has a higher affinity toward the GBD than does VCA, allowing it to displace the VCA and activate WASP. Next, I examined the role …
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RhoA and Rac1 prenylation: Effects on serine/threonine signaling for actin polymerization
… promoters (i.e association of WAVE with the ARP2/3 complex and activation of JNK and ERK), and inactivation of the actin-depolymerizing protein cofilin. Overexpressing non-geranylgeranylatable (EmGFP-RhoAC190A), but not wild-type RhoA (EmGFP-RhoA), increased activation of ERK in the cytosol …
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Biochemical and Biophysical Characterization of the Allosteric Equilibrium of the Wiskott-Aldrich Syndrome Protein
… of Cdc42 is coupled to WASP activation toward Arp2/3 complex-mediated actin polymerization, and 3) to understand the implications of the GTPase nucleotide switch on effector activation. In order to expand upon the range of WASP protein folding stabilities and to, more importantly, examine the …
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Identifying a role for WASH in the endocytic pathway of Dictyostelium discoideum
… WASP protein family are direct activators of the arp2/3 complex, thereby regulating the nucleation of branched actin assemblies within the cell. Each sub-class possesses a unique N-terminal domain architecture allowing a division of labour between its members, each coupling different signal …
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Processive Acceleration of Actin Barbed End Assembly by N-WASP
… the basement layer. In addition to activating Arp2/3 complex, N-WASP binds actin filament barbed ends, and both N-WASP and barbed ends are tightly clustered in these invasive structures. We used nanofibers coated with N-WASP WWCA domains as model cell surfaces and single actin filament imaging …
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Phase Transitions of Multivalent Adaptor Proteins
… of multivalent proteins that causes these complexes to phase separate in solution. The behavior of multivalent proteins and their ligands to phase separate may be a general property that allows cells to regulate their activities in certain localized compartments. To study this larger goal, …
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Physics of Self-assembly in Complex Matter
… is based on my graduate research studying complex matter in multiple areas of biological physics. While the complex matter systems in each area differ vastly in scale and function, I use similar computational approaches on each to answer scientific queries about their structure and …
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The Role of FAM21 in the Regulation of Mitochondrial Dynamics
… including the actin-related protein 2/3 (Arp2/3)-complex, have been implicated at different stages of the complex multi-step mechanism of mitochondrial division. Recent work has also highlighted the contribution of phosphatidylinositol 4-phosphate (PI4P) trafficking, from trans-Golgi …
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Linking WRP/srGAP3 to the Cognitive Deficits in 3p- Syndrome and Its Role in the Regulation of Dendritic Filopodia Formation
… model systems for critical genes in the WAVE complex pathway, including a conditional WRP KO mouse developed in our lab. Finally, because WRP is implicated in mental retardation, behaviors of WRP heterozygous and null mice have been evaluated.</p><p>This study shows that WRP's IF-BAR domain …
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Molecular mechanisms of Sorting nexin-9 in membrane triggered actin polymerisation
… sensing to trigger actin polymerisation via the Arp2/3 complex. While membrane binding is key in in vitro purified reconstitutions of actin polymerisation, the lipid specificity and contribution of each membrane domain is under dispute and not well understood in vivo. Three models have been …
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A large scale analysis of chemoattractant induced cytoskeletal dynamics in Dictyostelium discoideum by means of proteomic and imaging approach
… disorders and cancer metastasis. It is a complex cellular process which is composed of several steps including signal detection and amplification, cell polarisation and cytoskeleton reorganisation. There are several signal transduction pathways working in parallel to control chemotaxis, …
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Probing the regulatory mechanisms of the actomyosin system in motile cells
… of the signaling process: the regulation of Arp2/3 activity by WASP (actin system), the role of cGMP in myosin II assembly (myosin system), and the influence of phosphoinositide signaling (upstream receptor pathway). The model organism chosen for this work was the social ameba Dictyostelium …
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Investigating FAM83H, a protein mutated in Amelogenesis Imperfecta
… cytoskeleton via interaction with the WASP/WAVE/Arp2/3 complex and interaction with p21 activated kinase 1 (PAK1). The interaction of NCK with FAM83H requires the SH3 domains of NCK, which suggests that FAM83H could be an effector molecule in one (or more) tyrosine kinase signalling pathway(s), …
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