Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Argonaute Proteins"”.
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RNAi-related genes in Toxoplasma gondii.
… triggers degradation of homologous transcripts. Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNAi effector complex, named RNA-induced silencing complex (RISC). Our group previously reported that dsRNA-induced specific gene silencing is …
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Small RNAs Regulate Transcription by Interacting with Noncoding RNA Transcripts
… targeted duplex RNA has been shown to recruit argonaute proteins to the promoter DNA and these proteins are necessary for duplex RNAs to regulate transcription. Argonaute proteins are known to recognize RNA:RNA interactions. However, genes targeted with duplex RNAs have no characterized …
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Sexually dimorphic Argonaute localization underlies sex-specificity of small RNA pathways during germ cell development
… during germ cell development using functional Argonaute (AGO) proteins. AGOs mediate gene expression by both licensing genes required for meiosis and epigenetic inheritance, while simultaneously silencing deleterious genomic elements. The function and localization of AGOs during C. elegans …
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Regulation of APOBEC3 activity and HIV-1 replication by P-body associated proteins
… regarding the anti-viral nature of these proteins, their cellular function, as well as mechanisms of functional regulation and cellular co-factors, remains poorly defined. To address this, several cellular proteins that interact with APOBEC3G (A3G) have now been identified. The most …
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The Role of Arabidopsis thaliana Clade A1 Heat Shock Transcription Factors in Growth and Development
… an interaction between HSFA1b (or HSFA1s) and ARGONAUTE proteins was established suggesting another pathway by which HSFA1s could regulate developmental genes under both normal and stress conditions via the action of microRNAs. Alongside confirming HSFA1b-regulated developmental genes, the QK2 …
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Development and applications of programmable DNA-guided Argonaute-based artificial restriction enzymes
… defined sticky ends of varying length. Argonaute proteins are a family of nucleic acid guide-dependent proteins which can be found in all domains of life. Some prokaryotic Argonaute proteins (pAgos) are able to use short single-stranded DNA molecules as guides to target complementary DNA …
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Dynamic regulation of DNA demethylation and RNA-directed DNA methylation in Arabidopsis
… study in which we focused on AGO6 and AGO4, two Argonaute proteins involved in RdDM. AGO6 is generally considered to be redundant with AGO4 in RdDM. However, our genome-wide DNA methylation profiles and immunofluorescence localization analyses showed that redundancy between AGO4 and AGO6 is …
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Functional analysis of the Argonaute family proteins during early embryonic and germ cell development in the mouse
Argonaute proteins are known regulators of gene expression through their association with small non-coding RNAs. Specifically in mammals, microRNAs (miRNA) and short interfering RNAs (siRNAs) bind to Ago-like subclade of the Argonaute family to regulate gene expression, while Piwi-interacting RNAs …
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Dynamic Shuttling of AGO2 to the Nucleus Relieves MicroRNA Repression
The Argonaute (AGO) and the Trinucleotide Repeat Containing 6 (TNRC6) family proteins are the core components of the mammalian microRNA-induced silencing complex (miRISC). This machinery mediates post-transcriptional silencing of mRNA targets by sequence-specific hybridization with microRNAs …
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The Mechanism of Double-Stranded RNA Response in Neurospora
… in post-transcriptional gene silencing. Argonaute proteins play a critical role in this conserved pathway, which is present in protists, fungi, plants and animals. In addition, dsRNA can trigger the interferon response as part of the immune response in vertebrates. In this study, we show …
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An RNA-interference-based platform for genome analysis and engineering in Saccharomyces cerevisiae
… evolution is mostly confined to individual proteins, due to the lack of efficient tools to introduce mutations globally and iteratively on a genome scale. Thanks to its simplicity and effectiveness, the pooled RNA interference (RNAi) screening should satisfy the requirement of directed …
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A functional role for transfer RNA-derived microRNAs in human B cells
… biogenesis, physical association with Argonaute proteins, and the ability to repress mRNA transcripts in a sequence-specific manner. Specifically, CU1276 represses endogenous expression of RPA1, a gene with critical functions in many aspects of DNA dynamics, including replication and …
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Building the Human Argonaute 2 Interaction Network
… and translation in the cytoplasm. However, Argonaute (AGO) proteins, which are the major catalytic component of the RNA-induced silencing complex (RISC), have been found to be involved in nuclear roles outside of the canonical RNAi pathway. Within non-mammalian systems such as yeast and …
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Genome engineering in saccharomyces cerevisiae
… evolution is mostly confined to individual proteins, due to the lack of efficient tools to introduce mutations globally and iteratively in a genome. In the rest of this dissertation, I sought to develop a new method, RNA-interference assisted genome evolution (RAGE), to apply directed …
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The Role of TNRC6 in RNA Interference
… (RNAi) pathway. TNRC6, also known as GW182, and Argonaute (AGO) are the core proteins of RNAi. TNRC6 is a scaffolding protein that associates with AGO and bridges its interactions with other proteins to control gene expression in many different processes. There are three paralogs in mammalian …