Global ETD Search
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Showing 1 to 20 of 20 for “"Argonaute (Ago)"”.
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Biochemically informed modeling of miRNA targeting efficacy
… (miRNAs) are short pieces of RNA that load into Argonaute (AGO) proteins and base-pair to complementary sequences in mRNAs. Upon binding an mRNA, AGO- miRNA complexes recruit machinery that translationally represses and degrades the mRNAs. Mammalian genomes encode hundreds of miRNAs, and most …
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Understanding microRNA targeting with high-throughput biochemistry
… (miRNAs) are short RNAs that, in complex with Argonaute (AGO) proteins, guide repression of mRNA targets. miRNAs negatively regulate most mammalian mRNAs, and disruption of this regulation often results in severe defects at the cellular and organismal level. miRNA repression occurs primarily …
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Elucidating the Role of RanBP2/Nup358 in the Regulation of Cytokines mRNAs
… in ANE1. We showed that RanBP2 SUMOylates Argonaute (AGO) proteins, enhancing their interaction with target mRNAs and their binding partner GW182 to promote miRNA-mediated silencing of cytokine mRNAs. This repression depends on RanBP2’s localization at the nuclear pore, supporting a model …
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Building the Human Argonaute 2 Interaction Network
… and translation in the cytoplasm. However, Argonaute (AGO) proteins, which are the major catalytic component of the RNA-induced silencing complex (RISC), have been found to be involved in nuclear roles outside of the canonical RNAi pathway. Within non-mammalian systems such as yeast and …
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Genome-Wide Decoding of mRNP and miRNA Maps
… By generating crosslinking of native Argonaute (Ago) protein-RNA complexes in mouse brain, Ago HITS-CLIP produced two simultaneous datasets—Ago-miRNA and Ago-mRNA binding sites—that were combined with bioinformatic analysis to identify miRNA-target mRNA interaction sites. We validated …
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Dynamic Shuttling of AGO2 to the Nucleus Relieves MicroRNA Repression
The Argonaute (AGO) and the Trinucleotide Repeat Containing 6 (TNRC6) family proteins are the core components of the mammalian microRNA-induced silencing complex (miRISC). This machinery mediates post-transcriptional silencing of mRNA targets by sequence-specific hybridization with microRNAs …
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The Role of TNRC6 in RNA Interference
… (RNAi) pathway. TNRC6, also known as GW182, and Argonaute (AGO) are the core proteins of RNAi. TNRC6 is a scaffolding protein that associates with AGO and bridges its interactions with other proteins to control gene expression in many different processes. There are three paralogs in mammalian …
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Regulatory Mechanism of the RNAi Pathway
… siRNA guides the endoribonuclease Argonaute (Ago) to catalyze sequence-specific cleavage of complementary mRNA. It remains unclear as to what constitutes holo-RISC, how is RISC assembled and how is RISC regulated. Here we took a candidate approach to reconstitute for the first time …
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RNA Deregulation in Metastatic Breast Cancer
… For the post-transcriptional regulatory protein Argonaute (AGO), which binds mRNA in complex with small non-coding microRNAs (miRNAs), HITSCLIP derived 3'UTR binding sites have been shown to correspond to sites of functional miRNA-mediated target down regulation. In this study, a molecularly …
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Regulation of microRNA degradation in Caenorhabditis elegans via the E3 ubiquitin ligase EBAX-1
… repression through their associated Argonaute (AGO) proteins. Animal genomes encode hundreds of miRNAs that, together, regulate a majority of mRNAs and tune spatiotemporal gene expression programs. The production and degradation of many miRNAs occurs in a regulated manner, but …
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Regulation of miRNA degradation
… gene expression by guiding the effector protein Argonaute (Ago) to complementary sites in the 3′ UTRs of target genes. The levels of a miRNA in a given cell-type are determined by the balance of its rates of production and decay. Although much is known about miRNA production, relatively little is …
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RNA Dynamics in T Cell Activation
… We hypothesize that RNA-binding proteins such as Argonaute (Ago) and HuR play critical roles in shaping the dynamic immune response in T cells. We have previously demonstrated the ability to map Ago-microRNA binding sites using high-throughput sequencing of RNA isolated by cross-linking …
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Mechanism and Biological Scope of Target-Directed MicroRNA Degradation
… are small, ~21 nucleotide RNAs that guide Argonaute (AGO) proteins to complementary target RNAs using base-pairing interactions. In canonical miRNA-mediated repression, AGO recruits repressive trans-acting factors to bound targets to promote their deadenylation, decapping, and subsequent …
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A Genomic Portrait of Hepatitis C Virus and MicroRNA-122
… (HITS-CLIP) experiments of human Argonaute (AGO) during HCV infection. We demonstrate robust AGO binding on the 5' untranslated region of HCV RNA at known and predicted miR-122 sites, thereby establishing conclusive biochemical evidence of endogenous miR-122 action on HCV RNA that …
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Studies of Resistance to Aphids and Viruses in Arabidopsis thaliana and Capsicum annuum
… thaliana plants carrying mutant alleles for Argonaute (AGO) 1 to determine if it was due to accumulation of a cyanogenic compound, 4-hydroxyindole carbonyl nitrile (4-OH-ICN). Exogenous application of 1 mM SA to pepper plants prior to CMV inoculation induced resistance to CMV, which was …
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Modulation of Ago-miRNA regulatory networks by cis-sequence elements and target competition
… and biological processes. Each miRNA guides Argonaute (Ago) protein complexes to target and repress hundreds of genes in a sequence-dependent manner. To identify all targets of miRNA regulation, we performed UV crosslinking and immunoprecipitation (CLIP) of Ago complexes in mouse embryonic …
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Investigation sur la fonction du gène AGO5 dans le développement des plantes et la défense antivirale
… médié par les petits ARNs, et les protéines Argonaute (AGO) sont des endoribonucléases essentielles au bon fonctionnement de ce mécanisme. Les protéines AGO reconnaissent et se lient à des duplexes de petits ARNs, provenant de la transformation d'ARN double brin (ARNdb) endogènes ou viraux, …
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Transcriptome-Wide Characterization of APOBEC1-Catalyzed RNA Editing Events in Macrophages
… immunoprecipitation (HITS-CLIP) of the Argonaute (Ago) proteins was performed on wild-type and APOBEC1-deficient cells. HITS-CLIP yielded transcriptome-wide maps of Ago binding and potential miRNA seed regions. While there was considerable overlap between loci targeted by both Ago and …
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Molecular requirements for FMRP and RNA helicase MOV10 in the translational regulation of co-bound mRNAs
… FMRP has been known to directly associate with Argonaute (AGO), a key effector protein of the RNA induced silencing complex (RISC). MicroRNAs regulate key cellular processes in mammalian genomes by post-transcriptionally regulating gene expression. However, FMRP's role in microRNA-mediated …
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Biochemical and cell biological analysis of the mechanism of RNA interference in human cells
… Einzelsträngige siRNAs und miRNAs werden in Argonaute-Proteine enthaltende, sogenannte RNA-Silencing-Komplexe eingebaut, welche die Inaktivierung der komplementären mRNAs durch Spaltung oder durch translatorische Hemmung vermitteln. Es gibt acht Argonaute-Proteine, die im Menschen exprimiert …