Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 64 for “"Argonaute"”.
-
Building the Human Argonaute 2 Interaction Network
… and translation in the cytoplasm. However, Argonaute (AGO) proteins, which are the major catalytic component of the RNA-induced silencing complex (RISC), have been found to be involved in nuclear roles outside of the canonical RNAi pathway. Within non-mammalian systems such as yeast and …
-
Development and applications of programmable DNA-guided Argonaute-based artificial restriction enzymes
… defined sticky ends of varying length. Argonaute proteins are a family of nucleic acid guide-dependent proteins which can be found in all domains of life. Some prokaryotic Argonaute proteins (pAgos) are able to use short single-stranded DNA molecules as guides to target complementary DNA …
-
RACE-Seq identifies the Argonaute-2 cleavage products of RNA interference-based oligonucleotides
… by Dicer or incorporate directly into the Argonaute-2 RNA-induced silencing complex (AGO2-RISC) and directs the protein complex to homologous RNA. Cleavage of target RNA occurs opposite bases 10-11 when counting from the 5’ end of the hybridized siRNA guide strand. The capture and …
-
Functional analysis of the Argonaute family proteins during early embryonic and germ cell development in the mouse
Argonaute proteins are known regulators of gene expression through their association with small non-coding RNAs. Specifically in mammals, microRNAs (miRNA) and short interfering RNAs (siRNAs) bind to Ago-like subclade of the Argonaute family to regulate gene expression, while Piwi-interacting RNAs …
-
The interactions of the cucumber mosaic virus 2b protein with the viral 1a and host Argonaute 1 proteins
… interaction with the host RNA silencing factor Argonaute 1 (AGO1). The consequent inhibition of AGO1 activity induces developmental symptoms such as stunting and organ deformation in CMV-infected plants. In *Arabidopsis thaliana*, if 2b-induced inhibition of AGO1 is too strong this de-represses …
-
Discovery and biochemical characterization of RNA interference in budding yeast
… are then loaded into the effector protein Argonaute to guide the cleavage of target transcripts. RNAi and related RNA-silencing pathways are found in plants, animals, fungi, and protists, suggesting origins in an early eukaryotic ancestor and selective pressures to maintain the pathway. A …
-
RNAi-related genes in Toxoplasma gondii.
… triggers degradation of homologous transcripts. Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNAi effector complex, named RNA-induced silencing complex (RISC). Our group previously reported that dsRNA-induced specific gene silencing is …
-
Regulation of APOBEC3 activity and HIV-1 replication by P-body associated proteins
… identified. The most intriguing of these are the Argonaute proteins, as the interaction is at least partially resistant to RNase treatment. The Argonautes are integral components of RISC, which is involved in miRNA mediated translational repression and mRNA decay. Components of this pathway, as …
-
Small RNAs Regulate Transcription by Interacting with Noncoding RNA Transcripts
… targeted duplex RNA has been shown to recruit argonaute proteins to the promoter DNA and these proteins are necessary for duplex RNAs to regulate transcription. Argonaute proteins are known to recognize RNA:RNA interactions. However, genes targeted with duplex RNAs have no characterized …
-
Biochemical and cell biological analysis of the mechanism of RNA interference in human cells
… Einzelsträngige siRNAs und miRNAs werden in Argonaute-Proteine enthaltende, sogenannte RNA-Silencing-Komplexe eingebaut, welche die Inaktivierung der komplementären mRNAs durch Spaltung oder durch translatorische Hemmung vermitteln. Es gibt acht Argonaute-Proteine, die im Menschen exprimiert …
-
PERPETUATION OF NON-GENETIC CHANGES AT A TRANSGENE LOCUS IN C. ELEGANS
… the conserved dsRNA importer SID-1 and the Argonaute RDE-1, mating-induced silencing required the Piwi-interacting Argonaute PRG-1. Both processes engaged the same germline Argonaute HRDE-1 to maintain heritable silencing suggesting that both processes trigger silencing independently but …
-
The Mechanism of RNA Interference in Neurospora
… recognition and cleavage of the target mRNA by Argonaute. In biochemical experiments, Argonaute generates single-stranded siRNA by cleaving the passenger strand of the siRNA duplex. Mutational analysis of Neurospora homologue of Argonaute-2, known as Quelling Deficient -2 (QDE-2), revealed that …
-
The Function and Mechanism of RNA Interference in Neurospora
… silencing complex (RISC), in which an Argonaute family protein, guided by a siRNA, mediates the cleavage of homologous RNAs. In the filamentous fungus Neurospora, we show that dsRNA not only trigger RNAi, it also transcriptionally activates several key components of RNAi pathway, …
-
Functional study of miRNA-mRNA interactions in malaria mosquito An. gambiae
… miRNAs expression and their association with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) in the malaria mosquito Anopheles gambiae (An. gambiae) during the 72-h period immediately after blood feeding. I found the abundance and Ago loading of most of the mature miRNAs were relatively stable after …
-
Dynamic Shuttling of AGO2 to the Nucleus Relieves MicroRNA Repression
The Argonaute (AGO) and the Trinucleotide Repeat Containing 6 (TNRC6) family proteins are the core components of the mammalian microRNA-induced silencing complex (miRISC). This machinery mediates post-transcriptional silencing of mRNA targets by sequence-specific hybridization with microRNAs …
-
The Mechanism of Double-Stranded RNA Response in Neurospora
… in post-transcriptional gene silencing. Argonaute proteins play a critical role in this conserved pathway, which is present in protists, fungi, plants and animals. In addition, dsRNA can trigger the interferon response as part of the immune response in vertebrates. In this study, we show …
-
Biochemically informed modeling of miRNA targeting efficacy
… (miRNAs) are short pieces of RNA that load into Argonaute (AGO) proteins and base-pair to complementary sequences in mRNAs. Upon binding an mRNA, AGO- miRNA complexes recruit machinery that translationally represses and degrades the mRNAs. Mammalian genomes encode hundreds of miRNAs, and most …
Page 1 of 4