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Showing 1 to 20 of 43 for “"Arginase"”.
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Arginase from Bacillus caldovelox
… in arginine-supplemented media and screened for arginase, arginine deiminase and arginine decarboxylase activity by a reversed-phase liquid chromatography method developed to detect these arginine-catabolizing enzymes in cell-free extracts. Thermus, Bacillus and Sulfolobus strains produced …
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ISOLATION AND CHARACTERIZATION OF ARGINASE ISOZYMES FROM BOVINE LIVER AND BRAIN
… chromatographic method was developed to isolate arginase isozymes from bovine brain and liver. The purified proteins were characterized as to their native molecular weight, subunit structure, amino acid composition, hexose content, behavior on disc gel electrophoresis, and reaction with …
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Signaling Cross-Talk Regulating the Expression of Arginase 1 in Murine Macrophages
… resolution) phenotype. Expression of arginase 1 in macrophages is a key marker of the M2 phenotype. Arginase 1 expression is induced by interleukin 4 (IL-4), a cytokine secreted by Th2 helper cells. All-trans retinoic acid (ATRA) is a product of metabolism of dietary retinol (vitamin …
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Structural Studies of Two Related Metallohydrolases: Human Histone Deacetylase 8 and Malarial Arginase
… α/β protein fold first identified in rat liver arginase. Despite insignificant overall amino acid sequence identity, these enzymes share a strictly conserved metal-binding site with divergent metal specificity and stoichiometry. HDAC8, originally thought to be a Zn2+-metallohydrolase, exhibits …
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An investigation into Toxoplasma gondii infection and the expression of Arginase-1 and iNOS in human lung tissue
… the expression of the immune modulating proteins Arginase-1 and iNOS in human lung biopsy samples. Previously, a 100% toxoplasmosis infection rate was found in 72 lung biopsy samples acquired from patients. This compared with 10% in healthy controls. Little is known of immune mechanisms acting on …
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Immunological control of toxoplasma gondii infection
… adaptive immunity. Parasite induced macrophage arginase-1 expression, for example, has been shown to be influential during T. gondii infection. Arginase-1 expression is negatively regulated by Map Kinase Phosphatase-2 (MKP-2), the second major aim of the project was to study the effect of MKP-2 …
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Investigation of genetic and epigenetic mechanisms of iNOS gene expression in infection of natural populations of Apodemus sylvaticus with the parasite Toxoplasma gondii
… animals. The DNA sequences of the iNOS and Arginase genes of A. sylvaticus were not available in the DNA databases. PCR amplification, based on development of primers, from related rodent sequences were used to build up a partial sequence of both genes. Additional use of a partial genome …
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Regulation of nitrogen metabolism in vibrio alginolyticus
… The inducible nitrogen catabolic enzymes arginase, alanine dehydrogenase and histidase were not subject to nitrogen catabolite repression. Various amino acids and ammonium ions stimulated the production of histidase and arginase. Urocanase and formiminoglutamate hydrolase were repressed by …
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Generation of nitric oxide and its protective and toxic actions in the gastrointestinal tract
… NO synthase inhibitors, but was reduced by the arginase inhibitors NG-hydroxy-L-arginine and L-ornithine. Thus, the predominant pathway of arginine metabolism involves arginase and ornithine transcarbamoylase, not NO synthase. Pretreatment of rats with NG-nitro-L-arginine promoted activation of …
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EFFECTS OF RED BLOOD CELLS ON NITRIC OXIDE BIOACTIVITY
… for converting L-arginine to NO and citrulline. Arginase as another enzyme converts L-arginine to urea and L-ornithine. Nor-NOHA as arginase inhibitor is supposed to block the arginase pathway so that increase NO production. However, our data suggest that nor-NOHA reacts with hemoglobin to form …
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THE EFFECTS OF ORAL ARGININE ON ITS METABOLIC PATHWAYS IN SPRAGUE-DAWLEY RATS
… is metabolized by multiple enzymes including arginase, nitric oxide synthase, arginine decarboxylase, and arginine:glycine amidinotransferase. Our lab has recently published a study on the physiological impact of oral arginine at a dose of 500 mg/kg/day administered for 4 weeks in male …
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Immunopathogenic mechanisms of non-healing cutaneous leishmaniasis caused by Leishmania amazonensis infection
… transport was detected, no measurable iNOS or arginase activities were observed in IFN-g-treated, infected MFs. These data suggest an involvement of a novel L-arginine usage independent of iNOS and arginase activities during IFN-g-mediated parasite growth enhancement. The ability to scavenge …
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Ameliorative effect of the methanolic Crude extracts of Inula glomerata and Salacia kraussii on erectile dysfunction in Sprague Dawley rats
… uric acid as well as acetylcholinesterase, ACE, arginase activities were assessed. The crude extracts’ cytotoxicity was also determined using MTT assay. vii The result showed that both plants contain tannins, flavonoids, terpenoids, and alkaloids. At a varying degree of efficiency, the crude …
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Erectogenic activity of the Maytenus procumbens roots extract in type 2 diabetic rats
… (IC50 values ranging from 0.02 and 0.1 mg/ml), arginase (IC50 = 0.1 mg/ml), AChE, and ACE activities. The in vivo study further confirmed the erectogenic potential of the methanolic extract as it effectively lowered the activities of arginase, AChE and ACE along with increased serum testosterone …
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Investigating the relationship between lung cancer and the infection with the parasite Toxoplasma gondii
… have demonstrated the involvement of iNOS and Arginase gene expression in T. gondii infection. Nothing is known about the role of iNOS and Arginase in human lung tissue infected with Toxoplasma. We have established and optimised an IHC protocol to detect iNOS and Arg-1 in lung cancer samples …
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Immune related gene expression of parasite-resistant sheep during a Haemonchus contortus infection
… expressed Th2 genes such as interleukin-4 and arginase-1. Results from these two experiments indicate an adaptive immune response as early as 3 days post challenge infection in hair sheep. Thus, the rapid protective immunity generation of parasite-resistant hair sheep is a key difference by …
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Pathology and mechanisms of increased M.tb growth and dissemination during co-infection with HIV-1
… seen during co-infection. We have seen that arginase levels in the lung are highly and significantly correlated with pulmonary bacterial growth. However, the basis of this association is yet to be determined. We have also begun the adaption of our humanized mouse model for the study of …
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Biomolecules that impact phenotypic transitions in fibroblasts and macrophages
… are associated with this response. Increased arginase activity, increased iNOS activity, and morphological changes, indicating phenotypic changes in macrophages, were seen when CS-A, CS-B, CS-C, and CS-E were cultured with RAW 264.7 macrophages of five different phenotypes.</p>
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DNA analysis of Ornithine Transcarbamylase (OTC) deficiency in South African patients
… mental status changes. The fifth defect, arginase deficiency presents as progressive spastic quadriplegia and mental retardation but with milder elevation of blood ammonia levels. The molecular genetics of these disorders in South Africans has not been explored and there is thus very …
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