Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Arf1"”.
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Characterization of the GBF1-Arf1 axis in enterovirus RNA replication
… we sought to characterize the role of the GBF1-Arf1 axis in enterovirus replication. First, we systematically investigated the conserved elements of GBF1 to understand which determinants are important to support poliovirus replication. We demonstrated that multiple GBF1 mutants inactive in …
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Beteiligung der Phospholipase D an der Regulation der Synthese von Phosphatidylinositol-4,5-bisphosphat durch Arf- und Rho-GTPasen
… and PIP-5-kinase are stimulated by the GTPases Arf1 and RhoA, we investigated in the following, if PLD is involved in the regulation of PIP2 synthesis by these GTPases. Both PLD1- and PLD2-induced PIP2 synthesis was completely blocked by coexpression of catalytically inactive Arf1 T31N. …
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PI(4)P-Dependent Recruitment of Clathrin Adaptors to the Trans-Golgi Network
… on the small GTPase ADP ribosylation factor 1 (Arf1), but paradoxically, Arf1 has a broader intracellular distribution than these adaptors. We found that the Golgi is particularly enriched in phosphatidylinositol 4 phosphate [PI(4)P] and that the clathrin adaptor AP-1 binds PI(4)P directly, …
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Charakterisierung der peroxisomalen ARF-Bindung
… bei Säugern bekannten ARF-Isoformen konnten ARF1 und ARF6 an der Peroxisomenmembran identifiziert werden. Funktionelle Studien zeigten, daß ARF1 nicht jedoch ARF6 für die Rekrutierung von Coatomer an Peroxisomen essentiell ist. Dabei modulieren ATP und Faktoren aus dem Cytosol in …
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Cellular Prion Protein (PrPC): Identification and Characterization of Novel Interacting Partners
… partners of the GTPase family (Rab7a and Arf1) which have a suggested role in vesicle trafficking as well as the cytoskeleton associated protein alpha-tubulin 1 were further investigated using confocal laser scanning microscopy and reverse co-immunoprecipitation. Both reverse …
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Novel interactions of the 5-HT₂ₐ and related receptors with intracellular signalling proteins
… with ARF. The 5-FIT₂ₐ receptor associates with ARF1, and the third intracellular loop domain of the M3 muscarinic receptor associates with both ARF1 and ARF6, as shown by in vitro GST interaction assays.
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Proteomic analysis of the sorting machineries involved in vesicular traffic between the biosynthetic and endosomal compartments
… recognize their target membranes by activated Arf1 GTPases, organelle specific phosphoinositides, PI-4P and PI-3P respectively, and distinct cargo molecules carrying intact signals in their cytoplasmic domains. The implication of PI-3P in AP-3 recruitment was further supported by in vivo …
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Submembrane cytoskeleton-regulated assembly and functional activity of gap junctions
… Aktivität der kleinen GTPasen Sar1 und ARF1 ab. Der Transport von Cx43 vom endoplasmatischen Retikulum zum Golgi-Apparat wurde durch die Expression einer GTP-restrikten Mutante des Sar1 blockiert. Die GTP-gebundene Mutante der ARF1 verhinderte den Transport des Cx43 vom Golgi-Apparat zur …
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Regulatory domains of the Arf GEF Sec7 scaffold autoinhibited and active conformations
… at the TGN is dependent on the small GTPase Arf1 which drives vesicle formation and membrane modification following its activation by the Arf GEF Sec7. A network of inter- and intramolecular interactions of Sec7 gives robust spatiotemporal specificity to the potent Arf GEF. The Sec7 homodimer …
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A study of the mechanism of action of novel inhibitors of tumour cell invasion
… cells. By testing other genes from the list, ARF1 was identified as a target that partially mediates the anti-invasive activity in human cells. The results of this body of work show that Rho and ARF1 are important molecular players in the mechanism of tumour cell invasion. This knowledge will …
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Funktionelle Charakterisierung und ultrastrukturelle Lokalisierung der Isotypen des Hüllprotein-Komplexes Coatomer
… identifiziert werden. Zfp289 ist als Protein mit Arf1-GAP Aktivität beschrieben worden (Singh et al. 2001) und ist möglicherweise identisch mit ArfGAP2. Während eine Beteiligung der kleinen GTPase Arf1 und deren ArfGAPs an der COPI-Biogenese unstrittig ist (Palmer et al. 1993), ist der Einfluß von …
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Steps to Reconstitute in vitro a Complete Round of COPI vesicle Budding, Uncoating and Fusion
… in vitro based assay using soluble coatomer and ARF1 together with synthetic liposomes containing p23 tail peptide yielded generation of COPI vesicles, thus establishing the minimal requirements for COPI coat assembly (Bremser et al., 1999). In a program to reconstitute one round of budding, …
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Signal transduction by the 5-HT2A receptor and its H452Y polymorphic variant
… 5-HT2AR and H452Y-5-HT2AR receptor variant. Both ARF1 and PLD1 have previously been shown to bind to the carboxy-terminal tail (ct) of the 5-HT2AR. In chapter three it is demonstrated that the 5-HT2AR can activate PLD in an ARF-dependent manner, primarily through the PLD1 isoform. GST-fusion …
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Molecular basis of cargo sorting by endosomal trafficking machinery
… interaction sites for phosphoinositide lipids, Arf1 and dileucine cargo. Finally, I show that in vitro reconstitution of the AP-3 complex on synthetic membranes with PI35P2, Arf1 and cargo motifs is sufficient to drive assembly of highly ordered protein coated tubules, which may represent …
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Biochemische Analysen zur Funktion und Regulation von Cytohesin-1 in humanen T-Zellen
… Cytohesin-1 der Guanin-Nukleotid-Austausch an ARF1 in vitro reguliert wird. ARF-Proteine sind entscheidend an der Zytoskelettreorganisation beteiligt, die während Zelladhäsionsprozessen stattfindet. In Zellen zeigte sich, daß die LFA-1-abhängige Zelladhäsion an ICAM-1 durch phosphoryliertes …
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Effects of Exposure to Abuse and Violence in Childhood on Adult Attachment and Domestic Violence in Women’s Same-Sex Relationships
… heterosexual domestic violence (Gosselin, <a>[ARF1]</a> 2003; Niolon, 2002), it remains understudied. Therefore, this study not only examines the relationship between childhood abuse and intimate lesbian violence but also secure and insecure attachment styles. Children who witness domestic …
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Characterisation of new factors regulating mitochondrial dynamics and cellular metabolism
… not been fully elucidated. Here, Golgi-localised Arf1 and its downstream effector PI(4)KIIIß, were responsible for localised generation of the lipid PI(4)P on Trans-Golgi network vesicles, which are involved in mitochondrial division. Indeed, silencing of both genes led to a drastic mitochondrial …
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Studies of proteins implicated in the folding and processing of tubulin
… with human ADP ribosylation factor 1 (ARF1). Mining L. major genomic data identified putative ARF/ARL family members, which were examined and classified, based on conservation of amino acid sequences combined with what is known about orthologous proteins in Kinetoplastids and in humans, …