Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"AraC"”.
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Characterizing an AraC-Differentiated, Heterogeneous NT2-Derived Population
… research. Aim: The current study aims to characterize one such novel in vitro culture system that reduces these time and financial costs. Methods & Results: NTera2/cl.D1 (NT2) cells, a clonally-derived pluripotent human embryonal carcinoma cell line, are known to differentiate into …
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Mechanistic Studies of the Roles of the Transcriptional Activator ExsA and Anti-activator Protein ExsD in the Regulation of the Type Three Secretion System in Pseudomonas aeruginosa
… with host contact. ExsA, a member of the AraC-type transcription factor family, is the main transcriptional activator of all the genes necessary for expression of the T3SS. Members of the AraC family are characterized by the presence of two helix-turn-helix (HTH) motifs, which bind to the …
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Identifying Mechanisms Of Resistance And Potential Therapeutic Targets For Pediatric Acute Myeloid Leukemia
… cell lines, CMK and CMY, to the combination of araC and one of either the aurora A kinase inhibitor MLN8237, the aurora B kinase inhibitor AZD1152-HQPA, the Plk1 inhibitor BI6237, or the wee1 inhibitor MK-1775. It was found that MK-1775, in contrast to the other three agents, synergized with …
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Aberrant hippocampal neurogenesis contributes to learning and memory deficits in a mouse model of repetitive mild traumatic brain injury
… Previous findings in rodent models have characterized the effect of moderate traumatic brain injury (TBI) on neurogenesis and found learning and memory shortfalls correlated with limited neurogenic capacity. While there are no substantial changes after one mild TBI, research has yet to …
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Oncogenic roles of RAS in acute myeloid leukemia cooperated with Mll-AF9.
… disease (MPD)-like AML relapsed characterized by cells that did not express NRAS G12V . In comparison with primary AML, the MPD-like AML showed significantly reduced aggressiveness, reduced myelosuppression and a more differentiated phenotype. We conclude that, in AML induced by an …
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Proteindesign zur Verbesserung des Nucleosidanaloga-Umsatzes in menschlichen Zellen: Desoxycytidin-Kinase und UMP/CMP-Kinase
… mit dCyt und den zwei wichtigen Nucleosidanaloga AraC und Gemcitabin erhalten werden. Daraus ließen sich wichtige Informationen über die Bindung und Phosphorylierung dieser Substrate ableiten. Die UMP/CMP-Kinase konnte in der Substrat-freien Konformation kristallisiert werden. Dies erlaubte die …
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DNA -Binding Proteins That Facilitate Integration of Transmissible DNA Elements in Bacteroides Thetaiotaomicron
… both DNA elements. The proteins isolated and characterized include BHP, a non-specific DNA-binding protein that is most likely involved in nucleoid organization, BHP2, a homolog to BHP that probably functions in a similar capacity, and BT 1847, a specific DNA-binding protein that is a member of …
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Regulation der Genexpression bei der Erythroiden Differenzierung
… Leukämie (CML) mit verschiedenen Substanzen wie AraC oder Butyrat die Proliferation unterdrücken und eine weitere Differenzierung induzieren lässt. Bei CML-Zellen geht man davon aus, dass durch die genannten Chemikalien die Differenzierung in Richtung der erythroiden Reihe stimuliert wird, da …
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Switching in bacterial gene expression networks
… under identical growth conditions. We further characterized the differences within class 2 and class 3 gene expression and showed that a secretion-dependent feedback loop involving flagellar specific sigma factor, σ28, is responsible for partitioning cells into two fractions. Together, these …
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War Criminal, Multiculturalism, and Post-war Liberalism in Canada
In 1985, the federal government of Canada responded to concerns that Nazi war criminals were resident in the country by establishing a Royal Commission. Under the leadership of Judge Jules Deschênes the Commission of Inquiry on War Criminals was asked to investigate three questions: if war …
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The Role of Periplasmic Disulfide Bond Status in the Regulation of the Salmonella SPI1 Type Three Secretion System
… gene hilA is controlled directly by three AraC-like regulators: HilD, HilC and RtsA. A periplasmic disulfide bond oxidoreductase DsbA is required for SPI1 T3SS function. RtsA directly activates dsbA and deletion of dsbA leads to loss of SPI1-dependent secretion. We have studied the dsbA …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
Neurotransmitter release occurs by fusion of the synaptic vesicle membrane with the plasma membrane. Formation of a highly stable complex, known as the SNARE (soluble NSF-attachment protein receptors) complex, brings the two membranes close in space. SNARE complex formation is required but probably …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
Neurotransmitter release occurs by fusion of the synaptic vesicle membrane with the plasma membrane. Formation of a highly stable complex, known as the SNARE (soluble NSF-attachment protein receptors) complex, brings the two membranes close in space. SNARE complex formation is required but probably …
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Insights into the Molecular Mechanism of Streptomyces ipomoeae Pathogenesis: Thaxtomin C Biosynthesis and Regulation
… that of any storage roots it encounters. An AraC/XylS family transcriptional regulator TxtR encoded in the thaxtomin C gene cluster is shown here to be essential for S. ipomoeae pathogenicity, though the putative host-derived ligand that binds TxtR to activate thaxtomin C production remains …
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Examination of the role of early viral protein expression in MVA-induced NF-κB activation
… found that infection with MVA in the presence of AraC, a drug that inhibits viral DNA replication and intermediate and late gene expression, results in NF-κB nuclear translocation. However, under conditions where either viral entry is blocked, the virions are inactivated, or viral mRNA synthesis …
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Endothelin-1 is over-expressed in Amyotrophic Lateral Sclerosis and induces Motor Neuron cell death
… (ALS) is a neurodegenerative disorder characterized by progressive loss of motor neurons (MNs) and astrogliosis. Recent evidences suggest that factors secreted by activated astrocytes might contribute to degeneration of MNs. We focused on endothelin-1 (ET-1), a peptide which is strongly …
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Solution structure of unusual DNA sequences by high resolution NMR spectroscopy
… From my studies on d(CCAGGCCTGG) and d(CCAGGC (araC) TGG), I find that there are no significant differences between the two structures that can be localized to the arabinosyl cytosine residue or its neighbours but the arabinosyl moeity may be causing local rigidity that propagates beyond the …
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Novel regulators of antibiotic biosynthesis in Serratia sp. ATCC 39006
… sensing. This project aimed to identify and characterise new regulatory genes that modulate antibiotic biosynthesis in S39006. Novel regulators were sought through random transposon mutagenesis, using the wild type (WT) as parental strain and the prodigiosin production phenotype (red pigment) …
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Molecular Interactions of Type III Secretion System Transcriptional Regulators in Pseudomonas aeruginosa: ExsA and ExsD
… and an up-regulation of the T3SS. ExsA is an AraC/XylS-type transcriptional regulator and belongs to a subfamily of activators that regulate the T3SS in a variety of Gram-negative pathogens. These regulators are characteristically difficult to purify due to the low solubility of their …
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Rhamnose metabolism and metronidazole resistance in B. thetaiotaomicron VPI-5482
… displayed a MIC = 8 μg/ml was isolated and characterized. Southern hybridization was used to identify the insertion of a single copy of the transposon in an intergenic region of a gene cluster involved in the uptake and catabolism of L-rhamnose. RNA hybridization studies confirmed that the …
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