Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Aqueous two phase systems"”.
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Countercurrent chromatography of proteins using aqueous two-phase systems
… purify small organic molecules using aqueous - organic phase systems. Aqueous two - phase systems (ATPS) have been used historically to purify bio-molecules whilst retaining their biological function. This thesis focuses on extending CCC to the separation of proteins, using a model …
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Thermodynamics of salt-polymer aqueous two-phase systems: Theory and experiment.
A theoretical and experimental study of the phase behavior of aqueous salt-polymer two-phase systems has been done. A statistical mechanical model has been developed for the chemical potential of every component in the salt-polymer-water system. The model incorporates the effect of short-range …
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Neural Networks in Bioprocessing and Chemical Engineering
… principles and practical aspects of neural networks, focusing on their applications in bioprocessing and chemical engineering. This study introduces neural networks and provides an overview of their structures, strengths, and limitations, together with a survey of their potential and …
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Optimization of sequential purification of beta-glucosidase from tricoderma reesei in aqueous two-phase system
… with chitosan followed by separation with an aqueous two-phase system (ATPS), was implemented for the purification of beta-glucosidase from the culture filtrate of T. reesei. The cultivation medium (nutrient) was optimized for the production of betaglucosidase from T. reesei cell culture. …
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ABC terpolymers : micelles, polymersomes and stabilisation of water in water emulsions
… based on coupling the separation properties of aqueous two phase systems (ATPSs), which are able to provide w/w emulsions, with templated self-assembly of polymersomes. This novel method provides high encapsulation efficiencies of payload species which is effective, scalable and …
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Enantioseparation using a counter-current bioreactor
… by selective partitioning in the stationary phase of the CCC centrifuge, was accompanied by separation of unreacted L-ABA from KBA by the countercurrent action of the centrifuge. For effective bioreactor/separator action, a high partition of the biocatalyst to the stationary phase was …
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POLYELECTROLYTE NANOPARTICLE MEMBRANES AT WATER-WATER INTERFACES
… (NPs) and polyelectrolytes (PEs) on aqueous two-phase systems (ATPSs) provides a scalable route to assemble functional, stimuli-responsive membranes. The aim of this thesis is to identify the membrane self-assembly mechanism of such systems. We study two PE/NP systems in a dextran/PEG …
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Monoclonal antibody (mAb) purification by counter current chromatography (CCC)
… (mAb) from mammalian cell culture, using aqueous two-phase systems (ATPS) of inorganic salts and polymer. A polyethylene glycol (PEG) and sodium citrate system was found to be the most appropriate by robotic phase system selection. The search for an economical alternative to protein A HPLC …
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Termodinâmica e otimização da transferência de norbixina em sistemas aquosos bifásicos
Partição do corante natural norbixina ( ) foi estudado em sistemas aquosos bifásicos formados pela mistura de soluções de polímero ou copolímero com solução aquosa de sal (tartarato ou succinato de sódio). O coeficiente de partição do corante norbixina foi investigado como função do pH, massa molar …
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Termodinâmica de partição do corante natural carmim de cochonilha em diferentes sistemas aquosos bifásicos
Atualmente, corantes e pigmentos são largamente utilizados nas indústrias de alimentos, cosméticos e fármacos. Devido a diversos corantes sintéticos terem sidos considerados carcinogênicos, a demanda por corantes naturais tem crescido. Dentro deste contexto, o pigmento vermelho extraído do corpo …
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Self-assembly behavior in hydrophilic block copolymers
… were biopolymers or polymer chimeras used in aqueous two-phase partition systems. Copolymers based on dextran and poly(ethylene glycol) blocks were able to form aggregates in water. Dex6500-b-PEG5500 copolymer spontaneously formed vesicles with PEG as the “less hydrophilic” barrier and dextran …