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Showing 1 to 20 of 123 for “"Aptamer"”.
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Studying the Structural Dynamics and Aptamer-Ligand Interactions in the Cocaine-Binding Aptamer through Fluorescence Spectroscopy
Since its first report in 1990, aptamers have been utilized in biosensor modeling technologies. One of the most important advantages of using aptamers is the structural flexibility and thermal stability of nucleic acids. These structural merits enable aptamers to be linked on solid surfaces, …
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Aptamer Interactions and Applications in Cancer Treatment
Aptamers, small strands of RNA or DNA, are finding a rapidly expanding repertoire of applications. Aptamers have found numerous diagnostic and therapeutic uses both in vitro and in vivo. Specifically, their application in diagnosis and treatment of multiple cancers has been strongly advanced over …
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Methods of optimization for KCE-based aptamer selection
The success of KCE-based aptamer selection relies on three distinct steps of pre-selection optimization: (i) preventing protein adsorption to the inner walls of the capillary, (ii) maximizing the protein-DNA separation, and (iii) determining an appropriate aptamer-collection window. To perform the …
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Aptamer-based nano-scale dielectric sensor for protein detection
… nanoscale dielectric sensor functionalized with aptamer probes is developed. This work has been done in collaboration with Rational Affinity Devices LLC. The use of aptamer based detection offers several advantages over the traditional labor intensive antibody based immunosensing. In the initial …
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Development of an RNA aptamer to PD173955N through SELEX
… them less desirable for medical applications.;Aptamers are newly developed reagents and their utilization in medicinal applications is so far very limited. However their characteristics make them perfect tools to help solve problems associated with PD173955. Here we report a successful …
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An Optical Aptamer-Based Carbon Nanosensor for Cortisol Detection
… This work presents the development of an optical aptamer-based carbon nanosensor for the detection of cortisol. The sensor utilizes single-walled carbon nanotubes (SWCNTs) functionalized with a specific DNA aptamer to achieve high specificity and sensitivity. This work touches on the importance of …
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Aptamer-based Bioelectronic Sensors for Modular and Miniaturizable Molecular Monitoring
… electrochemically modified, structure-switching aptamers are incorporated onto the OECTs, enabled by a novel transistor operation method for kinetics-sensitive measurement. Finally, considerations for translation to point-of-care, blood-based applications are characterized. Through this …
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… by Exponential Enrichment (SELEX) method, RNA aptamers binding to the bacterial Tet Repressor protein (TetR) with low- to sub- nanomolar affinities were identified. This interaction is reversibly controlled by tetracycline in a manner analogous to the interaction of TetR with its cognate DNA …
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Achieving Cell-Specific Delivery of Multiple Oligonucleotide Therapeutics with Aptamer Chimeras
… somatic cells. </p><p>Advances in antibody and aptamer technology have enabled researchers to design those molecules to bind specifically to cancer cells, and deliver drugs that alter specific cellular processes. An aptamer designed to bind PSMA, a prostate cancer biomarker, only bound to a …
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Aptamer-Based Detection of Salivary Melatonin for Circadian Rhythm Sleep Disorders Monitoring
… in patients with circadian rhythm disorders. Aptamers, due to their numerous advantages such as small size and structural flexibility in tuning affinities, are preferable biorecognition molecules and can provide detection of melatonin with high sensitivity and specificity. The thesis aims to …
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2D layout of bead based thioaptamer/aptamer selection platform for therapeutics and diagnostics
… Especially, oligonucleotide agents (ODN or aptamers) are believed to affect cell function via complementary recognition or binding to specific proteins by forming tertiary structure. This opens new ways in therapeutics and diagnostics. The phosphoro- mono-/di- thioate substitutions in the …
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Mutational Analysis of HIV-1 Nucleocapsid Protein and Methods Development for Aptamer Discovery
… part focuses on the development of methods for aptamer discovery. Two prototype platforms were set up to imitate the two main-stream next-generation sequencing (NGS) technologies, bridge amplification based and emulsion PCR based NGS technologies. Proof-of-principle experiments were carried out …
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Immobilization Of The Anti-Thrombin DNA Aptamer: Attachment, Optimal Packing Density And Kinetics
Aptamers have been touted as viable complements to antibodies as molecular recognition elements in diagnostic assays. Several studies have demonstrated aptamer applicability in both homogeneous and heterogeneous assay formats. However, very few studies have been performed to investigate the …
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Aptamer-based sequence verification platform for rapid multiplexed detection of viral RNA targets
… and amplicon sequence verification using an aptamer-based colorimetric signal readout. With this method established, we then developed multiplexing detection platforms that target globally impactful mosquito-borne viral diseases and pathogens, including Dengue virus and Malaria, as well as …
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Catalysis by DNA enzymes: roles of lanthanide(III) ions and DNA aptamer modules
… possessing this ability are referred to as DNA aptamers and can also be identified via the in vitro selection process. Chapter 3 describes an effort to find DNA aptamers of ATP with a further goal of integrating these sequences into random DNA pools for deoxyribozyme selection. Providing a fixed …
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Optimizing a protein-RNA aptamer gene regulatory system using an engineered peptide library
… between proteins and proteinbinding RNA aptamers, takes advantage of the reversible interaction between TetR and its RNA aptamer binding partner 5-1.2 to modulate gene expression. With no tetracyclines present, TetR preferentially binds to aptamer 5-1.2 in the mRNA of a gene of interest …
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Discovery of Peptide and Aptamer Ligands for Targeted Drug Delivery to Hepatic Stellate Cells
… with monomer peptide-431. We also screened aptamer ligands using SELEX for targeted delivery to HSCs. In Chapter 4, IGFIIR-specific aptamers were identified using SELEX. The binding affinity and specificity of the aptamers were studied by flow cytometry and Surface Plasma Resonance (SPR). …
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Two-dimensional infrared spectroscopy of nucleic acids : application to tautomerism and DNA aptamer unfolding dynamics
… the structural dynamics of thrombin-binding aptamer (TBA), which is a 15mer DNA folded into a guanine-quadruplex (G-quadruplex). The 2D IR spectral signatures of G-quadruplex were established, and T-jump transient 2D IR was employed to investigate the unfolding dynamics of TBA. A mechanism of …
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The Cocaine-Binding Aptamer: A Thermodynamic and Structural Overview of Specific and Non-Specific Binding Interactions
… (Cp) among variants of the cocaine-binding aptamer were suggestive of two distinct binding mechanisms. Aptamer variants containing 6 base pairs in stem 1 are pre-folded and show little change in secondary structure with ligand binding. Aptamer variants with 3 base pairs in stem 1 are mostly …
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