Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 28 for “"Apoa"”.
-
Effects of phosphatidylinositol on ApoA-I metabolism: Implications in HDL metabolism
… (PI), have been shown to increase plasma apoA-I levels and HDL levels in animal models and human subjects; but the mechanism remains to be elucidated. Since in humans, HDL is primarily synthesized in the liver, the objective of the present study was to evaluate the underlying molecular …
-
ROLE OF APOLIPOPROTEIN A-V IN TRIGLYCERIDE METABOLISM
ApoA-V, a 366 amino acid protein synthesized by the liver, was identified in 2001 by comparative sequence analysis. The APOA5 gene is located downstream of the AI/CIII/AIV gene cluster on human chromosome 11q23. Apolipoprotein A-V (apoA-V) plasma concentrations are low (~100-200 µg/L) in comparison …
-
The Role of Lipid-Free Apolipoprotein A-I and PCPE2 in HDL Metabolism
… is to investigate the role of apolipoprotein apoA-I (apoA-I) and procollagen C-endopeptidase enhancer 2 (PCPE2) in high density lipoprotein (HDL) metabolism. ApoA-I comprises ~70 percent of the protein within HDL and has an essential role in cholesterol efflux from peripheral cells as part of …
-
IMPACT OF HEPATIC APOLIPOPROTEIN A-IV EXPRESSION ON VLDL PARTICLE EXPANSION, TRIGLYCERIDE SECRETION, AND STEATOSIS
… and secretion. Here we explore the impact of apoA-IV on VLDL particle expansion. Previous studies demonstrated that apoA-IV expression promotes apoB lipoprotein-mediated TG secretion in transfected enterocytes and hepatoma cells. We therefore examined the impact of apoA-IV expression on VLDL …
-
Molecular level characterisation of apolipoprotein A-I aggregation leading to fibrils comprising of both α-helical and β-sheet structures
… all of which contain characteristic features. ApoA-I, the main component in high-density lipoprotein, aggregates and becomes deposited as amyloid, either full-length apoA-I fibrils within atherosclerotic plaques, or N-terminal fragments of mutant apoA-I within organs. The work here aims to …
-
The Role of Apolipoprotein A-I in the Modulation of Cholesterol and Immune Homeostasis
The goal of this work was to examine the role of apoA-I in atherosclerosis and autoimmunity. We used a mouse model lacking the LDL receptor and the apoA-I gene (LDLr-/-,ApoA-I-/- or DKO). LDLr-/- (SKO) mice were used as controls. When fed a cholesterol-containing diet, DKO mice exhibited …
-
Structural and functional studies of the C-terminal domain of human apolipoprotein A-I: Limited proteolysis and deletion mutagenesis
… N-terminal fragment of human apolipoprotein A-I (apoA-I). Digestion of reconstituted high density lipoprotein (rHDL) prepared with apoA-I and dipalmitoyl phosphatidylcholine (DPPC) or palmitoyloleoyl PC (POPC) by chymotrypsin, trypsin, elastase, or subtilisin generated a major fragment of $\sim$22 …
-
Characterization of the Functional Role of the Intracellular Cholesterol Transporter StARD4 in Knockout Mice, and Investigation of Epigenetic Modulation of ApoA-I Transcription
… liver for excretion. The major protein of HDL is apoA-I and in mouse models it has been shown that animals transgenic for apoA-I have increased HDL levels. This suggests increased apoA-I transcription as a mechanism for increasing HDL, which might be preventive or therapeutic for coronary heart …
-
Lipid acquisition by apolipoprotein A-I in ER and Golgi compartments of primary mouse hepatocytes
… of intracellular lipidation of newly synthesized apoA-I in hepatocytes were in plasma HDL formation. By labeling primary mouse hepatocytes with 3H-choline, we showed that phospholipidation of apoA-I is most significant in endoplasmic reticulum (ER) and medial Golgi compartments with minor …
-
Caracterización bioquímica y genómica de las respuestas inducidas por lípidos de la dieta durante el desarrollo larvario del lenguado senegalés (Solea senegalensis, Kaup 1858)
… y caracterizado a nivel molecular la apoA-I, cuatro parálogos codificantes para la apoA-IV, tres parálogos para la apoB, la apoC-I, la apoC-II, dos parálogos para la apoE, cinco parálogos para la apoD y la apo14 en lenguado senegalés. El estudio de sintenia de las apoA, apoC, y apoE ha …
-
The study of spherical high-density lipoproteins and the intermediates involved in their formation
… discoidal rHDL, containing apo-lipoprotein A-I (apoA-I), cholesterol, and either palmitoyloleoyl-phosphatidylcholine or dipalmitoylphosphatidylcholine, with LCAT and low density lipoproteins. These spherical rHDL are characterized in terms of their composition, size, apoA-I structure and …
-
Transcriptional Regulation of Chicken Apolipoprotein A-I Gene Expression
… c-Jun in CEF, the chicken apolipoprotein A-I (apoA-I) gene has been identified in our laboratory as one of the target genes whose expression is repressed in response to v-Jun overexpression in CEF. The overall objective of this study is to investigate the underlying molecular mechanisms by …
-
An overview of blood-based biomarkers in AD
… AD, with a significant increase of cholesterol/ApoA-I and ApoD/ApoA-I ratios and reduced ApoA-II/ApoA-I ratio. Additionally, cholesterol/ApoA-I ratio is also positively associated to increased ventricular volume, while ApoA-II/ApoA-I and ApoJ/ApoA-I ratios are positively associated with grey …
-
Τα συστατικά στοιχεία του συστήματος λιπιδίων και λιποπρωτεϊνών ως κεντρικοί ρυθμιστές στην εμφάνιση της παχυσαρκίας και της μη αλκοολικής λιπώδους νόσου του ήπατος σε πειραματικά μοντέλα ποντικών
… εστιάσουμε στην μελέτη των απολιποπρωτεϊνών Α-Ι (apoA-I) και Ε (apoE) και του ενζύμου λεκιθινο-χοληστερολική ακυλοτρανσφεράση (LCAT). Η apoA-I αποτελεί το κύριο συστατικό των υψηλής πυκνότητας λιποπρωτεϊνών (HDL) και είναι υπεύθυνη για την σύνθεση τους, η LCAT εστεροποιεί την ελεύθερη χοληστερόλη …
-
Overexpression, purification and characterization of human proapolipoprotein A-I and mutants
The cDNA coding the human proapoA-I was cloned into an Escherichia coli vector, overexpressed and purified to 99% homogeneity and characterized together with apoA-I purified from human plasma. SDS-PAGE, mass spectrometry and Edman sequence analysis showed that the initial Met residue is post …
-
Synthetic efforts toward the eastern hemisphere of theonellamide C
… were not acceptable. The most recent approach to Apoa and Aboa utilized a key Horner-Wadsworth Emmons reaction followed by two conceptual sets of protecting group manipulations. The optimized conditions for the HWE reaction of (4S,5R)-tert-butyl …
-
The potential of sphingolipid depletion for the treatment of atherosclerosis
… myriocin may also be acting to increase hepatic apoA-I production via the inhibition of ERK phosphorylation. To address this, HepG2 cells and primary mouse hepatocytes were treated with myriocin. This significantly increased apoA-I mRNA, and protein levels. It also increased apoA-I secrection, …
-
Identifying circulating biomarkers in a cohort of hypercholesterolaemic patients with statin intolerance from Gauteng, South Africa
… analysis showed no significant difference in the ApoA-1 levels between participants in the control, statin-users, or statin naïve groups, (p= 0,4117). A significant difference between the CK levels between the different patient groups, p= <0,0001, was however noted with the average CK level for …
-
Molecular Basis of HDL-Mediated Endothelial Cell Migration and Reendothelialization
… injury is blunted in apolipoprotein A-I null (apoA-I-/-) mice, and reconstitution of apoA-I expression rescues normal reendothelialization. Furthermore, reendothelialization is impaired in SR-BI-/- mice. Thus, HDL stimulates endothelial cell migration via SR-BI-initiated activation of Rac …
-
Studies into novel therapies for the treatment of inflammatory artery disease
… the therapeutic efficacy of apolipoprotein A-I (ApoA-I), the major cardioprotective protein in high-density lipoproteins, and a class of immunomodulatory nanoparticles (INPs), which selectively target and disable a pro-inflammatory monocyte subset. Although ApoA-I treatment did not impact on the …
Page 1 of 2