Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"ApoA-I"”.
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Effects of phosphatidylinositol on ApoA-I metabolism: Implications in HDL metabolism
… (PI), have been shown to increase plasma apoA-I levels and HDL levels in animal models and human subjects; but the mechanism remains to be elucidated. Since in humans, HDL is primarily synthesized in the liver, the objective of the present study was to evaluate the underlying molecular …
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Characterization of the Functional Role of the Intracellular Cholesterol Transporter StARD4 in Knockout Mice, and Investigation of Epigenetic Modulation of ApoA-I Transcription
… liver for excretion. The major protein of HDL is apoA-I and in mouse models it has been shown that animals transgenic for apoA-I have increased HDL levels. This suggests increased apoA-I transcription as a mechanism for increasing HDL, which might be preventive or therapeutic for coronary heart …
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The Role of Lipid-Free Apolipoprotein A-I and PCPE2 in HDL Metabolism
… is to investigate the role of apolipoprotein apoA-I (apoA-I) and procollagen C-endopeptidase enhancer 2 (PCPE2) in high density lipoprotein (HDL) metabolism. ApoA-I comprises ~70 percent of the protein within HDL and has an essential role in cholesterol efflux from peripheral cells as part of …
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Molecular level characterisation of apolipoprotein A-I aggregation leading to fibrils comprising of both α-helical and β-sheet structures
… all of which contain characteristic features. ApoA-I, the main component in high-density lipoprotein, aggregates and becomes deposited as amyloid, either full-length apoA-I fibrils within atherosclerotic plaques, or N-terminal fragments of mutant apoA-I within organs. The work here aims to …
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The Role of Apolipoprotein A-I in the Modulation of Cholesterol and Immune Homeostasis
The goal of this work was to examine the role of apoA-I in atherosclerosis and autoimmunity. We used a mouse model lacking the LDL receptor and the apoA-I gene (LDLr-/-,ApoA-I-/- or DKO). LDLr-/- (SKO) mice were used as controls. When fed a cholesterol-containing diet, DKO mice exhibited …
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Structural and functional studies of the C-terminal domain of human apolipoprotein A-I: Limited proteolysis and deletion mutagenesis
… N-terminal fragment of human apolipoprotein A-I (apoA-I). Digestion of reconstituted high density lipoprotein (rHDL) prepared with apoA-I and dipalmitoyl phosphatidylcholine (DPPC) or palmitoyloleoyl PC (POPC) by chymotrypsin, trypsin, elastase, or subtilisin generated a major fragment of $\sim$22 …
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Lipid acquisition by apolipoprotein A-I in ER and Golgi compartments of primary mouse hepatocytes
… of intracellular lipidation of newly synthesized apoA-I in hepatocytes were in plasma HDL formation. By labeling primary mouse hepatocytes with 3H-choline, we showed that phospholipidation of apoA-I is most significant in endoplasmic reticulum (ER) and medial Golgi compartments with minor …
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The study of spherical high-density lipoproteins and the intermediates involved in their formation
… discoidal rHDL, containing apo-lipoprotein A-I (apoA-I), cholesterol, and either palmitoyloleoyl-phosphatidylcholine or dipalmitoylphosphatidylcholine, with LCAT and low density lipoproteins. These spherical rHDL are characterized in terms of their composition, size, apoA-I structure and …
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Transcriptional Regulation of Chicken Apolipoprotein A-I Gene Expression
… c-Jun in CEF, the chicken apolipoprotein A-I (apoA-I) gene has been identified in our laboratory as one of the target genes whose expression is repressed in response to v-Jun overexpression in CEF. The overall objective of this study is to investigate the underlying molecular mechanisms by …
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Overexpression, purification and characterization of human proapolipoprotein A-I and mutants
The cDNA coding the human proapoA-I was cloned into an Escherichia coli vector, overexpressed and purified to 99% homogeneity and characterized together with apoA-I purified from human plasma. SDS-PAGE, mass spectrometry and Edman sequence analysis showed that the initial Met residue is post …
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Τα συστατικά στοιχεία του συστήματος λιπιδίων και λιποπρωτεϊνών ως κεντρικοί ρυθμιστές στην εμφάνιση της παχυσαρκίας και της μη αλκοολικής λιπώδους νόσου του ήπατος σε πειραματικά μοντέλα ποντικών
… εστιάσουμε στην μελέτη των απολιποπρωτεϊνών Α-Ι (apoA-I) και Ε (apoE) και του ενζύμου λεκιθινο-χοληστερολική ακυλοτρανσφεράση (LCAT). Η apoA-I αποτελεί το κύριο συστατικό των υψηλής πυκνότητας λιποπρωτεϊνών (HDL) και είναι υπεύθυνη για την σύνθεση τους, η LCAT εστεροποιεί την ελεύθερη χοληστερόλη …
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An overview of blood-based biomarkers in AD
… AD, with a significant increase of cholesterol/ApoA-I and ApoD/ApoA-I ratios and reduced ApoA-II/ApoA-I ratio. Additionally, cholesterol/ApoA-I ratio is also positively associated to increased ventricular volume, while ApoA-II/ApoA-I and ApoJ/ApoA-I ratios are positively associated with grey …
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The potential of sphingolipid depletion for the treatment of atherosclerosis
… myriocin may also be acting to increase hepatic apoA-I production via the inhibition of ERK phosphorylation. To address this, HepG2 cells and primary mouse hepatocytes were treated with myriocin. This significantly increased apoA-I mRNA, and protein levels. It also increased apoA-I secrection, …
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Molecular Basis of HDL-Mediated Endothelial Cell Migration and Reendothelialization
… injury is blunted in apolipoprotein A-I null (apoA-I-/-) mice, and reconstitution of apoA-I expression rescues normal reendothelialization. Furthermore, reendothelialization is impaired in SR-BI-/- mice. Thus, HDL stimulates endothelial cell migration via SR-BI-initiated activation of Rac …
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Caracterización bioquímica y genómica de las respuestas inducidas por lípidos de la dieta durante el desarrollo larvario del lenguado senegalés (Solea senegalensis, Kaup 1858)
… y caracterizado a nivel molecular la apoA-I, cuatro parálogos codificantes para la apoA-IV, tres parálogos para la apoB, la apoC-I, la apoC-II, dos parálogos para la apoE, cinco parálogos para la apoD y la apo14 en lenguado senegalés. El estudio de sintenia de las apoA, apoC, y apoE ha …
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Studies into novel therapies for the treatment of inflammatory artery disease
… the therapeutic efficacy of apolipoprotein A-I (ApoA-I), the major cardioprotective protein in high-density lipoproteins, and a class of immunomodulatory nanoparticles (INPs), which selectively target and disable a pro-inflammatory monocyte subset. Although ApoA-I treatment did not impact on the …
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Factors affecting the binding of lecithin:cholesterol acyltransferase with interfacial substrates
… principal protein of HDL, apolipoprotein A-I (apoA-I) is a major physiological activator of the LCAT reaction. Despite the importance of this reaction, the factors which influence LCAT affinity for the surface of HDL are poorly understood. To determine what properties of HDL influence LCAT …
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Cholesterol Binding Activity of ApoAI Mimetic Peptide L4F
… or treating several cardiovascular disorders. ApoA-I mimetic peptides are promising candidates in this respect. L4F is a synthetic apoA-I mimetic peptide containing 18 L- amino acid residues of which four are phenylalanine and hence the name. It has been found to be capable of reducing lesions …
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Biomarcadores relevantes para diagnóstico precoce e prevenção de doenças cardiovasculares.
… of blood coag ulation. Levels of apoB and apoA - I represent effective atherogenic and anti - atherogenic parameters of cardiovascular diseases . Natriuretic peptides act in cardiovascular and renal physiology regulating blood pressure and blood volume . The predictive power of these …
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Pre-Clinical Development Of Aav Mediated Gene Therapy For Familial Lecithin Cholesterol Acyltransferase Deficiency
… response studies in LCAT KO and LCAT KO/ human ApoA-I transgenic mice using AAV8 expressing human LCAT. AAV8-TBG-hLCAT induces significant increases in plasma % cholesterol esterified at a dose as low as 3e9 GC per mouse, which is approximately equivalent to 1.5e11 GC per kg. We also …
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