Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Aplysia californica"”.
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Cardioregulatory Role of the Abdominal Ganglion of APLYSIA CALIFORNICA
<p>An isolated heart-abdominal ganglion preparation of this mollusc was developed and used to study the cardioregulatory function of the ganglion and nerves. The heart was perfused with artificial sea water through an atrial cannula and the gastro-esophageal artery was catheterized for blood …
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Modelling the synaptic plasticity underlying habituation, sensitization and classical conditioning of the Aplysia Californica Gill Siphon withdrawal reflex
Part I of this thesis aims to familiarize the reader with some of the basics of neuroscience. It includes a simplified description of the functioning of real neurons, as well as an introduction to several of the well known neuron models, including the Hodgkin Huxley equations and the Leaky …
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Analysis of D-Aspartate as a Signaling Molecule in the Aplysia Californica Central Nervous System Using Capillary Electrophoresis and Radioisotopic Labeling
… in the central nervous system. The mollusk Aplysia californica has shown high levels of D-Asp in the neural ganglia; and this, along with its relatively simple invertebrate system, makes Aplysia a viable model system for studying the function and neurochemistry of D-Asp. Using Aplysia …
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Amino acid racemase responsible for the biosynthesis of D-aspartate and D-serine signaling molecules in Aplysia californica central nervous system
… systems (CNS) of some marine mollusks including Aplysia californica, a model animal for cellular and systemic neuroscience study, and D-Asp showed neurotransmitter behaviors in Aplysia CNS, indicating D-Asp signaling pathway is conserved among animal kingdoms. Thus, studying D-Asp in Aplysia can …
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Novel Approaches for the Measurement of Unusual Post-Translational Modifications in Neuropeptides
… a D-amino acid-containing peptide found in Aplysia. These cells are then utilized in the development of a method combining the specificity of an enzyme with liquid chromatography/mass spectrometry to locate novel D-amino acid-containing peptides in the central nervous system of Aplysia …
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Off-Line CE/MALDI MS: Sampling and Deposition Techniques to Incorporate Complementary Analyses for Cellular Assays
… and single cells from the marine mollusc, Aplysia californica , assaying neuronal release, analyzing cone snail venoms, and incorporating radionuclide detection with CE/MALDI MS. The application of the CE/MS technique toward the analysis of these samples shows that this method is capable of …
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Neurotransmitter and Neuromodulator Analysis Using Capillary Electrophoresis With Laser -Induced Fluorescence Detection
… is involved in cell to cell communication in Aplysia californica. In addition, D-Glu is found in the sensory neurons of the sensorimotor synapse and induces a larger cellular response on the motor neurons than L-Asp. A method for single cell analysis of D-amino acid containing peptides in …
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Mass spectrometry-based investigations to characterize specific cell types within the brain
… this case, single cells from the model organism, Aplysia californica, are isolated for analysis. Mass spectrometry (MS) is well-suited for such applications because it has limits of detection in the attomole range for both small molecule metabolites and peptides, has a wide dynamic concentration …
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Neuropeptidomics Across Metazoan Life
… research areas. For example, the sea hare (Aplysia californica) is an excellent model to understand the formation, function and plasticity of neuronal networks, while the honey bee (Apis mellifera) is important for uncovering the mechanisms and evolution of social behavior. As studies in …
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Measurement of neuropeptides: From presence to release
… the questions about the presence of peptides. Aplysia californica cerebral neurons were used to verify predicted peptide processing of the putative prohormone AC2. However, the peptides were found to be absent in the forms given by the predictive software when analyzed with MALDI MS. Later …
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Analysis of enzymatic L/D-peptide isomerization in animals
… of DAACPs in the neurobiological model organism Aplysia californica. Two novel DAACPs were discovered using this method. Based on these results, it was hypothesized that an L/D-peptide isomerase exists in Aplysia which is responsible for the biosynthesis of DAACPs. An assay for isomerase activity …
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Immunocytochemistry, Mass Spectrometry and Microengineering for the Study of Cell Signaling
… by neuropeptide transmitters in the brain of Aplysia californica . This combination of techniques provides complementary qualitative information on the peptide structural identity, localization and action within a particular neuronal circuit. The study tests the hypothesis that specific …
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Transient intra- and extra-cellular thermometry as probes of thermogenesis
… mitochondrial proton uncoupling in neurons of Aplysia californica. We find that a transient proton motive force dissipation is more dominant than steady-state substrate oxidation in stimulated thermogenesis. Our measurements demonstrate the utility of transient intracellular thermometry in …
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Analysis of D-amino acid-containing neuropeptides in mollusks and rodents
… funnel is used to discover new DAACPs in the Aplysia californica nervous system and is applied, as pioneering work, to nervous system structures in rodents.
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Measuring D-amino acids in the central nervous system using capillary electrophoresis
… of the physiological responses it elicits within Aplysia californica (A. californica). Capillary electrophoresis (CE) is an ideal method to characterize D-AAs in the central nervous system due to the compatibility of CE with small size sample and the low detection limits it provides when coupled …
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Mass spectrometry imaging for probing molecular distributions in the nervous system
… cord from Rattus norvegicus and neurons from Aplysia californica. Mass spectrometry imaging (MSI) is a technique that allows for mass measurement of analytes, while also providing their spatial location and does not require a priori knowledge of the analytes to be imaged. MSI is capable of …
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Characterization of D-amino acid-containing neuropeptides from metazoa
… neurophysiological model organism the sea hare Aplysia californica, a suite of analytical methodologies have been integrated into a discovery funnel that allows identification of DAACPs from complex biological samples. With some modifications, these methods can be applied to a wide range of …
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Using microfluidics and mass spectrometry to study peptide release from neurons
… apply chemical stimulations to cultured Aplysia californica neurons within the device. By controlling the length of stimulation iv additions, the amount of stimulation necessary to enact release was determined for two different chemical stimulations, elevated K+ and insulin. A robust …
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Hyphenation of analytical techniques to single cell capillary electrophoresis-mass spectrometry
… enable RNA extraction from buccal ganglia from Aplysia californica with simultaneous delivery of an aqueous and organic phase. The biphasic extraction reduces the number of preprocessing steps, preventing sample loss, and successfully extracts suitable amounts of RNA for MS analysis. Finally, we …
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Novel molecular engineering approaches for genotyping and DNA sequencing
… long term memory studies in the model organism Aplysia californica. We reported the design, synthesis, and characterization of a binary probe (BP) that consists of carboxylic quantum dot (CdSe/ZnS core shell)-DNA (QD-DNA) conjugated donor and a cyanine-5 (Cy5)-DNA acceptor for the detection of a …
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