Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Antisense Oligonucleotide"”.
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Delivery and stability of antisense oligonucleotide conjugates in vitro
The efficacy of antisense oligonucleotide (ODN) therapy is dependent on four major parameters: delivery to cells, intracellular stability and localisation and efficient action at the target site.The aim of this project was to study the delivery of ODNs to macrophages and to assess the stability of …
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Thermodynamics and kinetics of antisense oligonucleotide hybridization to a structured mRNA target
Antisense oligonucleotides have the potential to selectively inhibit the expression of any gene with a known sequence. Antisense-based therapies are under development for the treatment of infectious diseases as well as complex genetic disorders. Although there have been some remarkable successes, …
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Role Of Gravin In Endothelial Wound Healing
… and a 96-well based cell migration assay using antisense oligonucleotide and siRNA treatments. The </p> <p>effect of antisense oligonucleotide and siRNA treatments on gravin expression was assessed by Western blotting and cell based ELISA. Finally, the reorganization of cortical actin band was …
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First evidence of skelemin, a myosin-associated protein, in smooth muscle and its involvement in cell adhesion, and, The role of the cell cycle in cell type choice during mammalian development
… may be more organized than has been assumed. Antisense oligonucleotide treatment was used to analyze the underexpression of skelemin protein in developing embryoid bodies. Skelemin underexpression was seen to cause loss of cell-cell as well as cell-substrate adhesion. Time lapse video …
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The Role of ACAT2 in the Initiation and Treatment of Atherosclerosis
… the treatment of atherosclerosis, we utilized an antisense oligonucleotide (ASO) to knockdown ACAT2 expression in the liver to determine how depleting atherogenic particles of CO would effect atherosclerotic lesion regression.
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Hypoxia-inducible factor-1 alpha (HIF-1alpha) and its role in the hypoxic preconditioning and gene expression patterns in zebrafish ( Danio rerio) embryos
… embryos deficient in HIF-1alpha (using antisense oligonucleotide morpholinos) were assessed under hypoxic conditions. No significant difference between preconditioning capacities of control- and HIF-1alpha deficient embryos was observed. In addition to assessing Pcrit, a suite of hypoxia …
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An investigation into the regulation and expression of the tumour suppressor gene clusterin in oral, cervical and nasopharyngeal cancer
… because of its anti-apoptotic function, with an antisense oligonucleotide currently undergoing clinical evaluation, results suggest that further consideration needs to be given to the possible tumour suppressor function of nCLU.
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SPINAL MUSCULAR ATROPHY ORGANOIDS REVEAL DEVELOPMENTAL DEFECTS RESCUED BY ANTISENSE OLIGONUCLEOTIDES TREATMENT
… hyperexcitability. Optimized peptide-conjugated antisense oligonucleotide tested in SMA SCOs successfully reverted both morphological and functional deficits. Of note, SMA cerebral organoids displayed similar functional deficits, revealing the widespread impact of the disease, beyond the spinal …
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Role of protein kinase C isoforms in human breast tumor cell survival
… MCF-7 and MDA-MB-231 human breast tumor cells by antisense oligonucleotides significantly impaired survival following radiation insult. Antisense oligonucleotides were shown to be effective and selective inhibitors of specific PKC isoforms. The PKC delta inhibitor, rottlerin reduced cell survival …
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Synthesis and bio-pharmacological activity of antisense phosphatidyl-oligonucleotides
Lipid conjugated oligonucleotides are of great interest in the field of antisense oligonucleotides used for functional genomics, gene target validation and therapeutic pourpose. Although various lipid conjugates of oligonucleotides have already been prepared, it was not possible until now to …
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Acute Manipulation of Erna Level For Dissecting Its Roles In Transcriptional Regulation
… based CRISPR activation or inhibition system and antisense oligonucleotide (ASO) based RNA inhibition system. I found that these CRISPR based systems show variable effects and sub-optimal acute perturbation of target eRNA, while ASOs are more acute for target perturbation. My study benchmarked …
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Blood-Derived Plasminogen Modulates the Neuroimmune Response in Both Alzheimer's Disease and Systemic Infection Models
… plasminogen in an AD mouse model through antisense oligonucleotide (ASO) technology lessened AD pathology and decreased glial cell activation in the brain, whereas an increase in plasmin activity through α2-antiplasmin ASO treatment exacerbated AD mouse pathology. Moreover, blood-derived …
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Trypanosoma Cruzi Phosphoinositide -Specific Phospholipase C (tcpi-Plc) and Its Role in Differentiation
… is involved in this differentiation step, antisense inhibition using phosphorothioate-modified oligonucleotides, and overexpression of the gene were performed. Antisense oligonucleotide-treated parasites showed a reduced rate of differentiation in comparison to controls, as well as …
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Investigating the roles of lncRNA TERRA in telomerase-to-ALT transition using cancer cell lines and multicellular organisms
… line in which we downregulated TERRA through antisense oligonucleotide (ASO) and measured changes in the ALT phenotype. In addition, we employed C. elegans mutant strains to study the dynamics of TERRA in worms lacking telomerase and elongating their telomeres using an ALT-like mechanism. We …
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Computational prediction of allosteric nucleic acids
… we built upon previous work that showed that antisense oligonucleotides bearing reporter moieties could be used to denature aptamers. Upon addition of ligands, the conformational equilibrium is shifted towards release of the antisense oligonucleotide and a concomitant increase in fluorescence. …
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Identification and Application of Novel Therapeutic Targets for PRPH2-Associated Disorders
… relevance of this strategy, we employed an antisense oligonucleotide (ASO), mRho ASO1, to reduce RHO levels in Prph2Y141C /+ mice. ASO intervention at multiple time points was able to successfully recapitulate the previously observed photoreceptor functional and ultrastructural improvements. …
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Deciphering The Role of Huntingtin In Endosomal Functions
… coding region of Huntingtin (HTT) gene. While antisense oligonucleotide (ASO) strategies aimed at lowering mutant HTT levels seemed promising, recent clinical trials were unsuccessful due to worsening patient outcomes in the ASO treatment group compared to the placebo. This may be attributed to …
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Design of Nuclear-Targeting Peptides for Macromolecule Delivery via Machine Learning
… design and characterize highly active CPPs for antisense oligonucleotide delivery were explored. Machine learning is a promising method for de novo design of functional peptide sequences. A deep learning model inspired by directed evolution was used to optimize abiotic sequences that traffic …
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The role of long non-coding RNA LINC00673 in lung tumorigenesis
… in cis. Interestingly, locked nucleic acid antisense oligonucleotide (LNA ASO) knockdown of LINC00673 enhances cell growth contrary to our hypothesis that the lncRNA functions as an oncogene. Knock-down of LINC00673 by CRISPRi and LNA ASOs suggest that downregulation of the lncRNA at the …
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