Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 260 for “"Antisense"”.
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Correlating antisense RNA performance with thermodynamic calculations
Antisense RNA (asRNA) strategies are identified as an effective and specific method for gene down-regulation at the post-transcriptional level. In this study, the major purpose is to find a correlation between the expression level and minimum free energy to enable the design of specific asRNA …
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Selective targeting of MYC by antisense oligonucleotides
… MYC. The goals of this research were to develop antisense oligonucleotides specifically targeted against the MYC mRNA to achieve potent inhibition of MYC translation, and to characterize the activity of these molecules as specific modulators of MYC expression and as prototypical MYC-directed …
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Enhancing spinal cord repair with connexin43 antisense oligodeoxynucleotides
… five days. The segments were treated with Cx43 antisense oligodeoxynucleotides (Cx43 AsODN) resulting in viable, organotypic, airliquid interface cultures that were then used for repair studies. Fresh peripheral nerves were grafted into the spinal cord segments with Cx43AsODN applied …
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CNS delivery of antisense oligodeoxynucleotides using biodegradable microspheres
Antisense oligodeoxynucleotides can selectively inhibit gene expression provided they are delivered to their target site successfully for a sufficient duration. Biodegradable microspheres have previously been developed for the potential systemic delivery of antisense oligodeoxynucleotides and offer …
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Synthesis and bio-pharmacological activity of antisense phosphatidyl-oligonucleotides
… are of great interest in the field of antisense oligonucleotides used for functional genomics, gene target validation and therapeutic pourpose. Although various lipid conjugates of oligonucleotides have already been prepared, it was not possible until now to prepare phosphatidyl …
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Delivery and stability of antisense oligonucleotide conjugates in vitro
The efficacy of antisense oligonucleotide (ODN) therapy is dependent on four major parameters: delivery to cells, intracellular stability and localisation and efficient action at the target site.The aim of this project was to study the delivery of ODNs to macrophages and to assess the stability of …
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Bioconjugate Strategies for Antisense Therapeutic Delivery to Glioblastoma Stem Cells
Antisense therapeutics, including antisense oligonucleotides (AONs) and small interfering ribonucleic acids (siRNAs), are powerful tools for regulating genes, making them a promising therapy for diseases such as cancer where oncogenic genes are over-expressed. The delivery of antisense therapeutics …
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Biodegradable polymers for the sustained release of antisense nucleic acids
Antisense oligodeoxynucleotides can selectively inhibit individual gene expression provided they remain stable at the target site for a sufficient period of time. Thus, the efficacy of antisense oligodeoxynucleotides may be improved by employing a sustained release delivery system which would …
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The sustained delivery of antisense oligodeoxynucleotides using biodegradable polymer microspheres
Antisense technology is a novel drug discovery method, which provides an essential tool for directly using gene sequence information to rationally design specific inhibitions of mRNA, to treat a wide range of diseases. The efficacy of naked oligodeoxynucleotides (ODNs) is relatively short lived due …
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Improving Cardiac Delivery of Antisense Oligonucleotides with Peptidomimetic Targeting Agents
… strategy. Here, we investigate the delivery of antisense oligonucleotides (ASOs) to inhibit the expression of an overexpressed miRNA in CVD, miRNA-21. One of the challenges in delivering ASOs and other gene therapies is achieving delivery to the desired tissue before the therapeutic is …
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Targeted 177 Lu antisense radiotherapy of B-cell non-Hodgkin's lymphoma
… to develop a [superscript 177]Lu-labeled bcl-2 antisense peptide nucleic acid (PNA)-peptide conjugate designed for dual modality NHL therapy, consisting of a radiopharmaceutical capable of simultaneously down-regulating apoptotic resistance and delivering cytotoxic internally emitted radiation. …
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Knockdown of nfa1 Gene Cloned from Naegleria fowleri by Antisense RNA
… cytotoxicity of N. fowleri to target cells, an antisense RNA or siRNA of nfa1 gene were transfected into N. fowleri trophozoites. By the synthetic dsRNA of nfa1 gene ORF, the expression of nfa1 gene and the Nfa1 protein were knockdowned about 50% and 30%, respectively. However, by antisense RNA …
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Peptide-mediated delivery of antisense oligonucleotides and chemotherapeutics across biological barriers
… methods for the peptide-mediated delivery of antisense oligonucleotides (ASOs) and chemotherapeutics. First, we address the issue that the optimal peptide sequence for the delivery of a macromolecular cargo is often context-dependent and specific to that cargo. With one class of ASO, we …
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SPINAL MUSCULAR ATROPHY ORGANOIDS REVEAL DEVELOPMENTAL DEFECTS RESCUED BY ANTISENSE OLIGONUCLEOTIDES TREATMENT
… hyperexcitability. Optimized peptide-conjugated antisense oligonucleotide tested in SMA SCOs successfully reverted both morphological and functional deficits. Of note, SMA cerebral organoids displayed similar functional deficits, revealing the widespread impact of the disease, beyond the spinal …
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USING ANTISENSE MESSENGER RNA TO DOWNREGULATE LON MEDIATED PROTEOLYSIS IN ESCHERICHIA COLI
… recombinant proteins. Consequently, a variety of antisense strategies have been examined and shown to effectively control endogenous gene expression and function in E. coli. For this investigation we explored using a 300 base pair sequence of the 5' coding region of E. coli lon gene, including the …
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DNA chip designed antisense oligodeoxynucleotides targeting EGFR MRNA for brain tumour therapy
… 40% of GBM. Novel forms of treatment such as antisense therapy may allow for the specific inhibition of aberrant genes and thus they are optimistic therapies for future treatment of GBM. Oligodeoxynucleotides (ODNs) are small pieces of DNA that are often modified to increase their stability to …
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Thermodynamics and kinetics of antisense oligonucleotide hybridization to a structured mRNA target
Antisense oligonucleotides have the potential to selectively inhibit the expression of any gene with a known sequence. Antisense-based therapies are under development for the treatment of infectious diseases as well as complex genetic disorders. Although there have been some remarkable successes, …
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Requirements and strategies for winning the battle against antibiotic resistance by antisense technology
… and conditions for efficiency and specificity of antisense molecules. For specific therapy, an antibacterial RNA must be able to distinguish between its designed targets and its off-targets. This distinction is reflected in the binding energy calculations. The major component of efficiency and …
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DNA methylation analysis and identification of a novel antisense transcript in the Peg3 imprinted domain
… this region for both CpG islands and long antisense transcripts, two common features of imprinted gene clusters. First, we performed a systematic survey of DNA methylation status of the CpG islands in this region of the mouse, cow, and human genomes. We identified two previously unreported …
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