Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"Antiport"”.
-
Functional expression and initial biochemical characterization of Yp-NhaP, cation-proton antiporter from Yersinia pestis
… of Yp-NhaP, putative sodium-proton antiporter from the dangerous human pathogen Yersinia pestis. We expressed Yp-NhaP in its functional form in the antiporter-deficient strain of E. coli, TO114. When assayed in inside-out sub-bacterial membrane vesicles, Yp-NhaP acted as an …
-
The design of synthetic transmembrane carriers for anions
… to function as efficient Cl-/NO3- and Cl-/HCO3- antiporters while the corresponding ureas were inactive. Of these receptors, a simple indolylthiourea was found to be an extremely potent transporter which could function at concentrations as low as 1:25 000 (molar ratio with respect to lipid). …
-
Non-Equilibrium Transport Across Liquid Membranes Using Coordination Cages
… mechanism to construct an artificial antiport system, driving nonequilibrium distribution of guest compounds temporarily, using the gradient of another guest as a driving force. This thesis provides strategic insight into molecular transport through coordination cages, which will be …
-
Neutral receptors for anion recognition: synthesis, selectivity studies and transport properties
… mechanism including Cl-/NO3- and Cl-/HCO3- antiport. Compound L14 was found to be the most active transporter of the series. As anion receptors, we observed that compounds L10 and L12 demonstrate strong 1:1 binding with TBA fluoride (Ka >104 M-1) and moderate 1:1 binding with TBA …
-
Energetics and mechanisms of substrate transport by the MATE transporter PfMATE
… PfMATE mediates electroneutral ethidium+ /H + antiport independent of membrane potential. Alongside with mutagenesis work, this investigation not only identified the essential carboxylates integral to PfMATE activity, but also revealed an alternative ion translocation pathway that can sustain …
-
Purification and characterisation of the Escherichia coli multidrug efflux protein MdtM
… vesicle transport assays illustrate the antiport activity of MdtM.<br/><br/>Site-directed mutagenesis studies of MdtM suggest that the residue D22 and the highly conserved residue R108 each play a role in proton recognition. MdtM was homologously overexpressed from a multicopy plasmid and …
-
On the chloride dependence of vesicular glutamate transport
… vom zellulären Kontext, einen Glutamat/Chlorid Antiport nachzuweisen, eine bislang nur vermutete Funktionsweise der vesikulären Glutamattransporter. Ausserdem konnte die Permeation von Chloridionen, unabhängig von einem Glutamattransport, durch den Tra nsporter selbst gezeigt werden, was die …
-
Energetics and Structural Aspects of Cation-Coupled Drug Transport by NorM Multidrug Transporters
… was shown to export drug (ethidium) in an antiport reaction that is coupled to the simultaneous uptake of protons and sodium ions down their electrochemical gradients across the plasma membrane. But NorM from Pseudomonas stutzeri (NorM-PS) was shown to transport DAPI by utilising proton …
-
Functional characterization of a putative Plasmodium falciparum calcium/hydrogen antiporter pfcha
… falciparum genome, a putative calcium hydrogen antiporter has been identified. To understand the role of this gene in the calcium metabolism of P. falciparum and to evaluate it as a drug target, we describe here the functional characterization by the heterologuous expression of this putative …
-
New Tools for Structural Biology and Biophysics: High-Throughput Fluorine Solid-State NMR and Applications to Membrane Proteins
… insight into the mechanism of the proton-coupled antiport.
-
Investigations into the Lipid Transport Mechanisms and Energetics of Bacterial ATP-Binding Cassette (ABC) Transporters MsbA and LmrA
… also shows a Na<sup>+</sup>/ethidium<sup>+</sup> antiport reaction that can be driven by the sodium-motive force, which when directed inwardly in cells gives rise to enhanced ethidium efflux, and when directed outwardly in proteoliposomes gives rise to ethidium uptake by purified inside-out …