Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Antibody staining"”.
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Immune Blocking of Fluorescent Antibody Staining of Streptococcus Group A Compared with Anti-streptolysin O
<p>The fluorescent antibody blocking antibody (FABA) test has been discribed and compared with the conventional antistreptolysin 0 antibody (ASOA) test in the detection of Streptococcus pyogenes group A antibodies in sera samples.</p> <p>A slide inhibition test was used to detect the presence of …
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A cell level automated approach for quantifying antibody staining in immunohistochemistry images. A structural approach for quantifying antibody staining in colonic cancer spheroid images by integrating image processing and machine learning towards the implementation of computer aided scoring of cancer markers.
… system for the automated quantification of antibody inside stained cell nuclei in immunohistochemistry images is proposed and demonstrated in this research. The system is based on a cellular level approach, where each nucleus is individually analyzed to observe the effects of protein …
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Rapid diversification and wing colour pattern evolution in tropical butterflies
… 3 through CRISPR-Cas9, in situ hybridisation and antibody staining. This chapter focuses on Mechanitis messenoides, but also contains work performed in Melinaea and Heliconius, all exploring the same colour loci. Chapter 5 studies the phylogeny of Ithomia, which has diversified slower than …
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Specific Roles of Kal-1 in Olfaction: A Model of Kallmann Syndrome in Drosophila
… which removes the entire kal-1 coding region. Antibody staining shows that Anosmin-1 specifically localizes to the dendrites of olfactory receptor neurons (ORNs) throughout development. The dendrites of ORNs sense odorants in the environment including the Drosophila melanogaster male-specific …
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ΣΥΓΚΡΙΤΙΚΗ ΜΕΛΕΤΗ ΤΗΣ ΧΟΡΙΟΓΕΝΕΣΗΣ ΣΕ ΕΝΤΟΜΑ ΟΙΚΟΝΟΜΙΚΗΣ ΣΗΜΑΣΙΑΣ
… ELECTROPHORESIS,AUTOFLUOROGRAPHY, SILVER STAINING METHOD OF GELS, IMMUNOCALIZATION OF PEROXIDASE WITH WESTERN BLOTTING AND DOUBLE ANTIBODY STAINING, PEROXIDASE LOCALIZATION IN THE LIGHT AND ELECTRON MICROSCOPE LEVEL USING THE O-DIANISIDINE AND DIAMINOBENZIDINE (DAB) METHODS, RESPECTIVELY.
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A study of treponemal antigens
… and T. refringens was studied using fluorescent antibody staining and those organisms were found to contain a group of common antigens. The cross-reactivity was removed when the antisera was absorbed with Reiter cells. There was a second group of common antigens that were shared by the subspecies …
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The Effects of Amphetamine Exposure on Neurodevelopment and Behavior in Early Life Stages of Danio Rerio
… deficits are further supported with fluorescent antibody staining in the spinal cord, where inhibitory expression was significantly higher in the high amphetamine dose compared to controls. This study using 24 and 48 hpf zebrafish offers a novel perspective on early amphetamine exposure during …
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Mutational analysis of JIL-1, a tandem kinase in Drosophila
… of the four mutations. Fluorescence-conjugated antibody staining of mutant chromosomes showed that for all four mutations the JIL-1 protein level was reduced and the localization of the JIL-1 staining was different from that of the wild type controls. Western blot analysis showed no detectable …
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Analysis of the Molecular Mechanisms of Drosophila Antennal Lobe Development
… to the disruption of the glomerular pattern. Antibody staining shows that Drl is located in the antennal lobe throughout the period of the development of the antennal lobe, from 16h after pupal formation (APF) to 40h APF and expressed by several types of cells including PNs, glial cells and a …
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Understanding HNF-1β through Identification of Interacting Proteins and Target Genes in the Kidney
… cAMP levels in mIMCD3 renal epithelial cells. Antibody staining showed that PDE4C is localized in the primary cilium and there interacts with a protein complex containing AKAP150, adenylyl cyclase 5/6 (AC5/6), protein kinase A (PKA), and polycystin-2 (PKD2). These results identify a …
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Development of amyloid beta binding bifunctional chelators for diagnosis of Alzheimer’s disease
… including ex vivo transgenic mouse brain section staining and autoradiography studies for assessment of amyloid plaques targeting ability, along with 64Cu radiolabeling studies and measurement of the octanol/PBS distribution coefficient (log D) values for prediction of blood-brain barrier (BBB) …
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Characterisation of a public T cell clonotype enriched in m.tb infected individuals who do not progress to tuberculosis
… kiif.tb T cells, quantified by custom monoclonal antibody (clone39a9d4) staining, were not different across study groups. However, active TB patients had higher frequencies of kiif.tb T cells expressing HLA-DR compared to IGRA- controls (p-value = 0.02). We did not include IGRA+ individuals, …
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Finding Common Ground: The Common Marmoset as a Model to Accessing and Providing Insight into the Social Brain
… characterize the entire region using iterative antibody staining with volumetric immunohistochemistry. From a genetic and circuits approach, social cognition has been implicated in numerous circuits across brain structures. One such circuit includes pyramidal neurons projecting from layer V of …
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Development and evaluation of methodologies for analysis of CTC and ctDNA in patients with ovarian carcinoma
… in this review (in Chapter 2), a multi-marker antibody staining protocol was developed to target epithelial (cytokeratin (CK) and EpCAM), mesenchymal (vimentin), and OC specific (PAX8) markers for detection of CTCs (Chapter 3). CD45/16 and CD31 were used for the exclusion of white blood and …