Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 19 of 19 for “"Antibody Engineering"”.
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Antibody engineering for cancer therapy
… cancer. In this Thesis, novel antibodies and antibody-conjugate against two tumor antigens, AF-20 antigen and human aspartyl (asparaginyl) [beta]- hydroxylase (HAAH), were developed. Previously, these two tumor antigens have been shown to be present on a variety of tumor cells, while they have …
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Antibody engineering for tumor immunotherapy
… therapy may be improved is the retention time of antibody in the tumor relative to normal tissue. In this Thesis, we have attempted to elucidate the mechanisms that are most influential to improving antibodies as cancer therapeutics. Carcinoembryonic antigen (CEA) has long been identified as a …
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Antibody engineering to evaluate binding, internalisation, and intracellular routing of tumour-targeting fusion proteins
… diseases, holds promise for precision medicine. Antibody-drug conjugates (ADCs) use antibodies to deliver drugs selectively. This study focused on a novel recombinant fusion protein format for ADCs using a single chain variable fragment (scFv) specific to chondroitin sulphate proteoglycan 4 …
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Antibody detection of Burkholderia pseudommallei and Burkholderia mallei
… found to be visually very similar. A monoclonal antibody raised to B. mallei LPS has been found to recognise both B. mallei and B. pseudomallei strains. However, in a small number of B. pseudomallei strains a visually atypical LPS profile has been demonstrated. A monoclonal ant ibody rai sed …
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Development of a Novel Bioconjugation Platform for the Generation of Homogenous Antibody-Drug Conjugates
Antibody-drug conjugates (ADCs) are therapeutic entities which leverage the specificity of antibodies to selectively deliver potent cytotoxins to specific cell types, such as cancer cells. The pharmacology of an ADC is critically dependent on its stability, homogeneity, and drug-to-antibody ratio …
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The Development of Divinyl-Heteroaryl Linkers for the Synthesis of Stable and Functional Antibody-Drug Conjugates
Antibody-drug conjugates (ADCs) are a class of targeted therapeutics that utilise the exquisite selectivity of antibodies for membrane receptors to selectively deliver cytotoxic warheads to specific cell types, such as cancer cells. Much effort has been invested in recent years to deliver ADCs with …
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Engineering the antibody-TRIM21 interface for the improved intracellular clearance of pathogenic targets
… However, limited mechanistic understanding of antibody action has constrained clinical success. Recent work shows that extracellular tau-antibody complexes can be internalised by neurons and degraded via the intracellular Fc receptor and E3 ubiquitin ligase TRIM21, and that passive tau …
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STUDIES OF B CELL DEVELOPMENT AND V(D)J RECOMBINATION
… responding B cells that ultimately give rise to antibody secreting plasma cells. Understanding the complexity of the recombination processes and the diversity of the resulting antibody repertoire has been a major focus of academic and industrial research alike. Therapeutic monoclonal antibodies …
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CHARACTERIZATION OF DENGUE NEUTRALIZING ANTIBODIES USING RECOMBINANT ANTIBODIES TECHNOLOGY
… FOR SUBSEQUENT VACCINE AND THERAPEUTIC ANTIBODY DEVELOPMENT AS WELL AS FOR TODAY?S EXPLODING IMMUNOTHERAPY RESEARCH. THE FIRST HALF OF THIS THESIS IS DEDICATED TO THE CLONING, EXPRESSION, AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES OBTAINED FROM TWO NATURALLY INFECTED DENGUE PATIENTS. …
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Study of a Transferrin Receptor Brain Shuttle for the Delivery of Nanobodies Against GABAA Receptors
… evident for biological drug classes, such as antibody-based therapies, contributing to their limited application in the treatment of neurological disorders. Technologies have emerged to improve drug delivery by targeting receptors present at the BBB, such as transferrin receptor (TfR), …
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Structural investigation of teleost immunoglobulin M tetramer assembly and engineering of chimeric antibodies consisting of teleost motifs
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-05-01
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Combatting pathogens through directed evolution, single-cell sequencing, and synthetic immunology
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-05-01
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In vitro diversification of chimeric human/chicken antibodies
… the system by finding an alternative approach to antibody humanization and evolution that overcomes the obstacles encountered during the evolution of human scFvs in DT40. I tested different approaches and, although presenting new challenges, I found that chimeric human/chicken antibodies could be …
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TUMOR-TARGETED RELEASE OF IMMUNOSTIMULATORY AGENTS: SYNTHESIS AND BIOLOGICAL EVALUATION OF IMIDAZOQUINOLINE-BASED PRODRUGS AND ANTIBODY CONJUGATES
… the different delivery vehicles proposed, antibody drug conjugates (ADCs) have been especially successful in the clinic. Recently, the involvement of the immune system in cancer treatment has been exploited. Following this strategy, immune-stimulating antibody conjugates (ISACs) have …
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Isolation and Characterization of Anti-SLP Single Domain Antibodies for the Therapy of C. difficile Infection
… enhances survival in hamsters, suggesting that antibody-mediated bacterial neutralization may be an effective alternative therapeutic strategy. Using a recombinant-antibody phage display library, and SLPs from strain QCD 32g58 as bait antigen, we isolated and extensively characterized 11 …
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The structure, biophysics and bioengineering of secretory immunoglobin A
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-12-01
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Development of Radiochemical Quality Control Methods for Ra-223 and Th-227 Radiotherapies
… this work through evaluation of a Th-227-labeled antibody construct. <strong>Conclusion.</strong> Our results develop a novel quality control protocol for Th-227 and Ra-223 on three different, readily available instruments. These results can be applied to both research and clinical settings as new …
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Characterisation of DNA i-motif structures in the human genome and the role of non-canonical nucleic acid structures
… By utilising the newly developed high-affinity antibody and conducting immunoprecipitation followed by sequencing to map i-motifs in the genomic DNA, we have mapped the possible forming locations. We validated these immunoprecipitated sequences, mapped locations by biophysical forms and …