Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Antibody Discovery"”.
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Development of a Versatile Antibody Cloning and Expression System
… In Biopharmaceutical communities specialised in antibody production, expression levels of grams per litre have been reached. However, lack of a suitable manufacturing platform, which ensures consistent antibody production, has always been one of the major impediments to the development of …
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Rational Design and Application of Antibodies for Amylin and Amyloid Beta
… and affinity for targets. However, traditional antibody discovery methods often fail to produce antibodies with desirable properties such as epitope selectivity, conformational stability, and solubility. Computational antibody design methods combining epitope targeting, solubility calculation …
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Site-Specific Labelling of the Flavivirus Envelope Protein using Genetic Code Expansion
… efforts to identify ZIKV-specific B cells for antibody discovery. Additionally, I explored how different labelling approaches might be used to probe dimer stability and the conformational dynamics of the virion. This work lays the foundation for novel approaches using genetic code expansion and …
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Methods to Study Antigen-Specific B cells
… a microfluidic platform for studying antibody secreting cells (ASCs) and a reporter mouse model that enables fate-mapping of stimulated B cells. Firstly, I introduce a screening platform for studying single ASCs. Studying ASCs with high throughput is inherently difficult due to the …
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Systematic development of rationally designed antibodies targeting predetermined epitopes of interest and protein aggregation
… Using a rationally designed αSyn antibody as a template for evolution, it was found that protein engineering via directed evolution and phage display is indeed capable of producing stable variants of the designed antibodies with improved affinity and crucially, retention of the …
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Developing Antibody Tools against Glycine Receptors
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Establishment of recombinant antibody technologies allowing for the generation of SNAP-tag fusion proteins
… have been conjugated to cytotoxic drugs to form antibody-drug conjugates, which may exhibit multiple advantages over their unconjugated counterparts, but their general use in clinical application has been restricted due to developmental deliberations. Historical conjugation strategies used for …