Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Anti-CRISPR"”.
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Mechanisms of bacterial CRISPR immunity and a phage counter-defense
… line of defense is the adaptive immune system CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats). Upon infection, the bacterial host uptakes a genetic memory from the bacteriophage to incorporate into its genome. This molecular memory is used to target CRISPR-associated (Cas) …
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Anti-CRISPRs and CRISPR-Cas: Characterization and Biotechnology
… systems, and the more recently discovered CRISPR-Cas immune systems. Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) loci together with their accompanying CRISPR-associated (Cas) genes form the only known bacterial adaptive defense mechanism that effectively protects …
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Mammalian genome engineering: Fundamental investigation and method development
… Activator-Like Effector Nucleases) and CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems are the most widely employed gene-editing tools presently. The modularity and specificity of these gene-editing proteins has made it possible to efficiently engineer …
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A novel CRISPR-Cas9 platform with divergent targeting capabilities
RNA-guided DNA endonucleases of the CRISPR-Cas system are widely used for genome engineering and thus have numerous applications in a wide variety of fields. The range of sequences that CRISPR endonucleases can recognize, however, is constrained by the need for a specific protospacer adjacent motif …
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THE CRISPRATOR: A FEEDBACK CONTROLLER IN MAMMALIAN CELLS FOR ROBUST PERFECT ADAPTATION
… applications. Gene networks implementing an Antithetic Integral Controller have only been presented very recently using mRNA pairs and inteins. In this thesis, I implemented the CRISPRaTOR, an Integral Controller based on the interaction between a CRISPR/Cas9-derived transcription factor and …
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Development of high-throughput nanoparticle screening technologies to facilitate the delivery of genetic therapies to non-liver cell types
Genetic drugs (such as siRNAs, mRNAs, and CRISPR/Cas9) have the potential to be curative therapies for countless diseases. However, gene therapies will only work if the genetic drug is delivered to the diseased cell type. One promising delivery method is through the use of Lipid Nanoparticles …
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Uncovering New Mechanisms of Type III CRISPR-Cas System Inhibition and Effector Function
… is the bacterial adaptive immune system called CRISPR-Cas, which consists of clustered, regularly interspaced short palindromic repeats (CRISPRs) and a set of genes that encode CRISPR-associated (Cas) proteins. While there are seven distinct types of CRISPR-Cas systems identified thus far, we …
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Development of Improved Genetic Methods for Enterococcus Faecium
… of drug resistance and nosocomial infections. Antibiotic usage has afforded antibiotic-resistant and pathogenic isolates from livestock and humans. Nevertheless, commensal Enterococcus strains possess beneficial health functions in mammals to upregulate host immunity and prevent microbial …
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Recombination and Excision: DNA Repair Proteins in Prokaryotic Host-Virus Conflicts
… tools, such as restriction enzymes and CRISPR-Cas9. My thesis investigates how mechanisms of DNA repair, specifically recombination and base excision, have been co-opted by phages and bacteria to execute non-canonical immune and counter-immune functions in prokaryotic host-virus genetic …