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Showing 1 to 20 of 81 for “"Annexin-V"”.

  1. Mechanism of Action of Antipsychotics, Haloperidol and Olanzapine in vitro

    Mahapatra, VijaylaxmiVirginia Tech2001

    … probes (7-aminoactinomycin D and Annexin-V) were employed to evaluate mechanisms of drug-induced cell death in PC-12 pheochromocytoma cells exposed to HP or Olz. At low dose (50 µM), HP was more cytotoxic than Olz. At high concentrations (150 mM) each of these antipsychotic drugs …

    vt Repository record

  2. The effects of jasmonates on the proliferation of human prostate cancer cell lines in culture

    Ezekwudo, Daniel Emeka

    … analyzed using flow cytometry (BrdU assay and Annexin V-FITC/PI staining) and fluorescence microscopy. The mechanism of inhibition was studied using 5-lipoxygenase enzyme. All agents (CJ, JA and MJ) inhibited proliferation of the cells in dose- and kinetic-dependent manners. All the assays …

    unlv Repository record

  3. Host cell-pathogen interactions between erythrocytes and P. falciparum: apoptotic mimicry?

    CHAN CHUU LING2013

    … were phagocytosed by THP-1 cells, addition of Annexin V did not inhibit phagocytosis. However, an increase in IL-6 production and a decrease in IL-10 production were observed, suggesting upregulation of pro-inflammatory signals through the blocking of PS. In conclusion, the role of PS still …

    nus Repository record

  4. The Effects Of Curcumin On The Expression Of P53 And Bcl-2 Oncoproteins On The Human Breast Carcinoma Cell Line MDA-MB 468

    Shajan, Angelin S.2015

    … and viability data were determined using an Annexin V and Dead Cell Kit, p53 expression was determined using a Multi-Colored DNA Damage Kit, and Bcl-2 expression was determined using a Bcl-2 Activation Dual Detection Kit. Results show a significant dose-dependent increase in both cell death …

    columbus-state Repository record

  5. Apoptotic markers in ejaculated human spermatozoa

    Brooks, Nicole LisaUniversity of the Western Cape2005

    … in ejaculate sperm. These assays included the Annexin-V staining for the determination of phosphatidylserine exposure, APO-Direct to identify DNA fragmentation, caspase-3 to detect expression of this active protease during early apoptosis and Fas expression. For the Annexin-V and caspase-3 …

    western-cape Repository record

  6. Development of novel isolation methodologies for microvesicles and exosomes, as potential biomarkers for health (Therapeutic Lifestyle Changes), and use of microvesicle-delivered β-Gly in erythroleukaemia

    Grant, Ryan ConradLondon Metropolitan University2016

    … relative ability to bind FITC and PE conjugated annexin V, anti-LAMP1, anti-CD63 and anti-CD11b antibodies using flow cytometry. Protein gels were run with MVs and exosome samples isolated by different techniques to identify some distinct proteins in MVs/exosomes that may be uniquely present on …

    london-metro Repository record

  7. Homing of 111In-Labeled Bone Marrow Mesenchymal Stem Cells in Acute Brain Trauma Model

    박, 복남2011

    … the 14th day. FACS analysis after staining with Annexin V- fluorescein isothiocyanate (FITC) and propidium iodide (PI) was performed at early (3 and 12 hr) and late (7 days) stages with higher doses of 111In (11.1 and 33.3 Bq/cell) to evaluate apoptotic or necrotic change of labeled BMSCs. The …

    ajou Repository record

  8. The Role of Neutrophil Apoptosis in Horses with Acute Abdominal Disease

    Krista, Kathryn MortonVirginia Tech2012

    … using a commercially available staining kit (Annexin V-PE Apoptosis Detection Kit I, BD Pharmingen™). The Mann-Whitney U test was used to detect significant differences in neutrophil apoptosis between the two groups as well as between lesion types in the abdominal surgery group. Correlations …

    vt Repository record

  9. Investigations on the effects of Typha capensis on male reproductive functions

    Ilfergane, AbdulkaremUniversity of the Western Cape2016

    … cell early apoptosis (determined by means of Annexin V-Cy3 binding), DNA fragmentation (determined by means of the TUNEL assay). Part 3: NMR spectrometric analysis was performed on a 13C spectra were recorded at 400 MHz. TM3-Leydig cells and LNCaP cells incubated with different concentrations …

    western-cape Repository record

  10. Production and Characterization of an Human Monoclonal Antibody IgG to TRAIL Receptor 2

    유, 화니2012

    … 정제하였으며 western blotting을 통해 확인하였다. MTT assay, Annexin V-FITC/PI staining, FACS analysis, sub-G1 분석을 통해 DR5-18 IgG가 림프 암세포 주 Jurkat 에서 apoptotic cell death를 유도하는 것을 확인 하였다. DR5-18 IgG는 Fc 분절을 가지고 있으므로 in vitro 상에서 IgG 단독 처리에 비해 보체와 함께 처리하였을 때 암세포 치사율이 더 높아짐을 확인하였다. 따라서 DR5-18 IgG는 성공적으로 만들어졌음을 …

    ajou Repository record

  11. Adaptation of Three Different Apoptotic Methods in Equine Bronchoalveolar Cells and Comparison of Bronchoalveolar Lavage Cell Apoptosis in Normal and COPD Affected Horses Before and After Dexamethasone Administration

    Leichner, Teri LynnVirginia Tech2001

    … (PI/Triton-X), 7-aminoactinomycin D (7-AAD), and Annexin V with propidium iodide (Annexin/PI) were evaluated in equine airway lymphocytes. A significant linear relationship was found for equine bronchoalveolar lavage (BAL) lymphocytes stained with 7-AAD and Annexin/PI . No relationship was …

    vt Repository record

  12. Gingko biloba extracts protect auditory hair cells from cisplatin-induced ototoxicity by inhibiting perturbation of gap junctional intercellular communication

    최, 성준2014

    … 24 h, and then analyzed by immunocytochemistry (Annexin V/ propidium iodide) and Western blots. The function of GJIC was evaluated by scrape-loading dye transfer (SLDT). Basal turn organ of Corti (oC) explants from neonatal (p3) rats were exposed to cisplatin (1–10μM) and EGb (50–400μg/ml). The …

    ajou Repository record

  13. TIS21 shifts p53-induced response of EJ bladder carcinoma cells from senescence to apoptosis

    최, 옥란2011

    … as demonstrated by staining with EthD-1 and Annexin V. Moreover, the expression of TIS21 increased apoptosis-associated acetylation of p53 molecule at lysine residues and its accumulation in the nuclei. Based on the evidences presented herein, I conclude that TIS21 directs p53-induced …

    ajou Repository record

  14. Effect of TIS21 on DNA Damage Repair Induced by Etoposide

    최, 규성2011

    … Caspase 3/7 activity, chromatin condentation and annexin V staining data suggest that TIS21 inhibited Chk2-p53 DNA damage pathway. Furthermore, TIS21 regulated PRMT1 activity and induced hypermethylation of Mre11 protein. In conclusion, TIS21 involved DNA damage signaling and inhibited Chk2-p53 …

    ajou Repository record

  15. Characterization of Cytotoxicity against Cancer Cells induced by Human Monoclonal Antibody Specific to TRAIL-R1

    이, 유리2011

    … cell death was confirmed by flow cytometry after Annexin-V/PI staining and DNA fragmentation assay. Caspase activation was measured by Apo-ONE caspase3/7 Assay kit, and cleavage of caspase-3 and PARP were detected by immunoblotting. To investigate mechanisms of DR4-4 Fab-induced cytotoxicity, …

    ajou Repository record

  16. Circulating microparticles: a risk factor for recurrent abortion in women at the University Teaching Hospital

    Chibuye., SylviaUniversity of Zambia2016

    … poor plasma by flow cytometry using fluorescent annexin V-fluorescence isothiocyanate conjugate.Microparticle levels in cases was compared to controls using unpaired t-test and logistic regression used to determine the parameters of the questionnaire associated with abortion. Increased …

    zimbabwe Repository record

  17. Circulating microparticles: a risk factor for recurrent abortion in women at the University Teaching Hospital

    Chibuye., SylviaUniversity of Zambia2016

    … poor plasma by flow cytometry using fluorescent annexin V-fluorescence isothiocyanate conjugate.Microparticle levels in cases was compared to controls using unpaired t-test and logistic regression used to determine the parameters of the questionnaire associated with abortion. Increased …

    zambia Repository record

  18. Verstärkung Butyrat induzierter Apoptose in kolorektalen Karzinomzellen durch Aspirin und Tumor Nekrose Faktor alpha

    Kudlich, TheodorUniversität Würzburg

    … diesem Zweck wurde ein durchflußzytometrischer Annexin V – Propidiumjodid – Assay etabliert. Mit Hilfe dieses Assays konnte gezeigt werden, daß der Apoptose induzierende Effekt sich sowohl durch eine Kombination mit Aspirin als auch durch eine Kombination mit TNFalpha im Sinne einer additiven …

    wurz-thes Repository record

  19. Caffeine Supplementation and Moderate Intensity Exercise Modulates the Cytotoxic Lymphocyte Subset (CD+8) in NaIve and Tolerant Individuals

    Fedor, Elizabeth Ann

    … Blood was stained with antibody markers (Annexin V to determine apoptotic cell counts, CX3CR1 to determine cell migration, CD4=helper T cells, CD8=cytotoxic T cells, CD19=B cells). Blood was analyzed using flow cytometry. We found that cytotoxic T cells showed significant increases …

    wku-diss Repository record

  20. Phospholipase A2 Induced Monocyte Chemotaxis to Apoptotic Cells

    Karikari, Kwasi2014

    … (CD-95) on Jurkat cells and monitored through annexin-V binding and propidium iodide(PI) staining followed by flow cytometric analyses. THP-1 monocytes are employed in chemotaxis assay with monocyte chemotactic protein (MCP-1) as a positive control. The preliminary data show equivalent group …

    vcu Repository record

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