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Using CRISPR/Cas9 to modify the genome of cattle

Lotti, Samantha NicoleUniversity of Illinois at Urbana-Champaign2017

… with increased milk production. MAC-T cells and Angus fetal fibroblasts were transfected with a Cas9 plasmid, pSpCas9(BB)-2A-GFP, and a ssODN to insert the desired mutation. Using the CRISPR/Cas9 system we were able to create a double strand break resulting in indels and deletions at the (+15) …

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