Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Ancestral sequence reconstruction"”.
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Reconstructing Cross-Species Ancestral Adeno-Associated Viruses for Enhanced Gene Therapy Delivery
… we developed a computational framework based on ancestral sequence reconstruction (ASR) to engineer synthetic ancestral AAV capsids with the goal of enhanced targeting specificity and potency. We first validated this computational framework by replicating the previously engineered Anc80L65 …
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Modeling Sequence Uncertainty in Comparative Genomics with a Probabilistic DNA Representation
Uncertainty in nucleotide sequences is widespread in bioinformatics, arising from somatic mutations, population-level variation, sequencing errors, and ancestral state inference. Yet, standard formats like FASTA encode DNA deterministically using ASCII string characters, omitting this uncertainty …
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Reengineering a human-like uricase for the treatment of gout
… computational and experimental approach of Ancestral Sequence Reconstruction to search functional sequence space of uricase proteins to engineer an enzyme with high sequence identity to the human pseudogene, and possessing therapeutic levels of activity for the breakdown of uric acid. This …
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The evolution of AroA and MurA enzymes from Bacillus
… is known about the evolution of AroA and MurA. Ancestral sequence reconstruction (ASR), a technique used to infer sequences of ancestral proteins and study their properties in the laboratory, was used to study the evolution of AroA and MurA enzymes from Bacillus. The ancestral AroA and MurA …
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The evolution and diversity of noncanonical microbial nitrogen metabolisms
… gene resulted from a chimeric domain fusion in ancestral Chloroflexi. I also identify an underreported variant of nitric oxide reductase (eNOR) within Chloroflexi MAGs, and provide a detailed phylogeny of this enzyme variant, revealing much broader diversity than previously reported. Next, I …
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Strategies to increase production of betalain pigments and their use as SynBio tools
… unsurpassed titres of betalains. Finally we used ancestral sequence reconstruction and enzyme activity assays in yeast to identify the residues important for high DODA activity in the Globular Inclusion clade, containing the Cactaceae. With this approach, we also confirm that the Globular …
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Breaking carbon-carbon bonds with cytochrome P450s from plants, animals, and bacteria
This Dissertation was approved for publication on 2022-04-19 at 13:48.
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Unsupervised inference models for structural and functional properties of protein sequences
L'abstract è presente nell'allegato / the abstract is in the attachment
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Molecular Evolution and Functional Characterization of the Visual Pigment Proteins of the Great Bowerbird (Chlamydera nuchalis) and Other Vertebrates
… to experimentally investigate the functional consequences of genetic variation in vertebrate visual pigments. The focus is on great bowerbirds (Chlamydera nuchalis), which are a model system in visual ecology due to their spectacular behaviour of building and decorating courtship bowers. There …