Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Amphiphysin"”.
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Functional Characterization of Amphiphysin in Drosophila melanogaster
Amphiphysin (Amph) is a multi-domain protein that has been implicated in synaptic vesicle (SV) endocytosis. In vertebrates, Amph1 associates with SVs and binds to known endocytic proteins, such as dynamin and clathrin. Overexpression of the vertebrate Amph1 SH3 domain is sufficient to inhibit SV …
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Lipid and Protein Sorting by, and Generation of, Membrane Curvature in Model Systems
… Finally, the curvature generation of Drosophila amphiphysin N-BAR domain is quantitatively assessed through the tube-pulling assay as before but performed using homogenous lipid membranes. Fluorescently-labeled protein preferentially sorted into the high (positive) curvature of the tubes from the …
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Understanding Membrane Curvature Sensing
… the method I tested proteins from the Bin/Amphiphysin/Rvs (BAR) superfamily, which are inherently curved and have known curvature preferences. My method was capable of recapitulating the behaviour of BAR domains with different curvature preferences. I then expanded the range of targets and …
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Computational investigations of protein dynamics and its implication in cellular functions: two cases on membrane sculpting by protein complexes and molecular origin of Parkinson's disease
… in eukaryotic cells. Proteins of the Bin/Amphiphysin/Rvs (BAR) domain family, in particular, play a role in cellular morphogenesis, for example curving planar membranes into tubular membranes. However, it is still unclear how F-BAR domain proteins act on membranes. Electron microscopy …
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Cell Biology of the ICA69 protein family in Neurosecretory cells
… paralogues containing the BAR domain, such as amphiphysin 1 and 2 or Rvs167p and Rvs161p, ICA69 and ICA69-RP were shown not to form heterodimers. Furthermore, ICA69-RP did not show any interaction with Rab2 or Rab1, involved in the anterograde transport between ER and Golgi. Thus, its …
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Pil1, an Eisosome Organizer, Plays an Important Role in the Recruitment of Synaptojanins and Amphiphysins to Facilitate Receptor-Mediated Endocytosis in Yeast
The eisosome protein Pil1 is known to be implicated in the endocytosis of Ste3, but the precise biological function of it during endocytosis is poorly understood. Here, I present data to reveal Pil1's role in receptor-mediated endocytosis. Using live cell imaging, I show that endocytic patches …