Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Amphipathic Helix"”.
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Biochemical and structural studies of the FtsZ:FtsA complex and polymerising abilities of the FtsA protein
… C-terminal motif, which is predicted to form an amphipathic helix and has been shown to localise FtsA (and with it FtsZ) to the membrane. FtsA belongs to the actin/HSP70 family of proteins, although its fold was shown to deviate by a subdomain deletion and addition. Many members of this family …
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Functional characterisation of the human mitochondrial ATP-Mg/Pi carriers and their disease-causing variants
… responsive to extra-mitochondrial calcium, an amphipathic helix, and a mitochondrial carrier domain, which catalyses substrate transport. In the proposed “locking-pin” mechanism, the amphipathic helix is bound to the regulatory domain in the presence of calcium, whereas it is bound to the …
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HAMP Domain and Signaling Mechanism of the Aer Protein
… The Aer HAMP domain is proposed to contain three amphipathic helices. The first amphipathic sequence (residues 207-233) was required for proper folding of the N terminus of Aer. Mutations that abolished FAD binding were clustered in the second putative amphipathic helix (residues 234-251), whereas …
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Holin-dependent secretion of a large clostridial cytotoxin by C. perfringens
… cell-surface biotinylation to determine if the amphipathic helix of TpeE changes its orientation in the presence of TpeL. Using FAST fluorescence, the mean pixel intensity for each frame did not change between TpeE-FAST and TpeE-FAST TpeL cultures, using a membrane impermeable dye. This …
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The Role of DNA Binding in Transcription Activation by Estrogen Receptor
… of a well defined, synthetic 18 amino acid amphipathic helix into the hinge region of the Xenopus estrogen receptor (XER). The resulting mutant, XER/2AH, provides us with a useful tool to study the mechanisms of gene activation by estrogen. XER/2AH exhibited 2-3 fold increased …
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Structural characterization of chaperone and substrates from the type three secretion system of bacterium aeromonas hydrophilla
… region of both translocators and the amphipathic helix region of AopD. We have also successfully shown complex formation between all three proteins AcrH-AopB-AopD.
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Mutagenic effects on protein folding and stability
… core, electrostatic interactions, turns and helix-capping motifs, and secondary-structure propensity have been shown to be important for folding and stability to varying degrees. The pattern of polar and nonpolar residues (binary pattern) in a sequence appears to be most critical for …
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Molecular Mechanisms of DNA Protection by Tardigrade Damage Suppressor Protein (Dsup): Structural Adaptation and Functional Interactions
… flexible N- and C-terminal regions and a central amphipathic helix forming a helix-turn-helix motif and highly charged C-terminal domain. The C-terminal domain, enriched in positively charged residues, mediates strong electrostatic interactions with DNA, while the helical region stabilizes the …
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Analysis of the interaction between the 2C protein of parechoviruses and lipid droplets
… we found that other domains, including a novel amphipathic helix are important. The results suggest that lipid droplets play a role in HPeV replication and so we investigated the effect of drugs which target lipid droplet formation and lipid homeostasis on HPeV replication. We demonstrated that …
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Activation of lipid kinase VPS34 by Rab GTPases
… While the autophagic complex I relies on an amphipathic helix in ATG14L for membrane association, the endocytic complex II uses mainly three aromatic loops in the Beclin 1 subunit to engage with membranes. I have found that Rab GTPases act as specific regulators for each complex. While Rab5 …
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Regulatory domains of the Arf GEF Sec7 scaffold autoinhibited and active conformations
… dimerization interface. We show dimerization, an amphipathic helix in the HDS1-2 linker, and switching to the active conformation modulates membrane avidity. We therefore conclude the GEF domain is in equilibrium between active and autoinhibited conformations governed by the affinity of the …
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Characterization of structure and dynamics of membrane proteins from solid-state NMR
… close proximity to water, indicating the post-amphipathic helix (AH) cytoplasmic domain is a dynamic random coil near the membrane surface. The influenza M2 ectodomain contains highly conserved epitopes but its structure is largely unknown. The M2(1-49) construct containing both the ectodomain …
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Bioinformatics Discovery And Functional Characterization Of Lipid-Binding Lov Photoreceptors
… likely driven by a conserved lipid-binding amphipathic helix, may serve to potentiate RGS effector activity on membrane-bound binding partners in the native fungal organism. As neither LOV photoreceptors nor RGS proteins nor PAS sensory proteins are known to traffic by light-gated and direct …
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Structure and dynamics of full-length M2 protein of influenza A virus from solid-state NMR
… domain (TM) (22-46) connected to an amphipathic helix (AH) and a Cterminal cytoplasmic tail (63-97). The TM domain of M2 is responsible for proton conduction ant the ectodomain has been the target for vaccine development. The cytoplasmic tail has been implicated in M2 interaction with …
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The Role Of The Membrane Proximal Region Of The M2 Cytoplasmic Tail In Virus Replication
Influenza A virus encodes M2, a proton channel that has been shown to be important during virus entry and assembly. The primary aim of this thesis was to investigate the role of the membrane proximal region, residues 46-69, of the M2 cytoplasmic tail during virus replication. A cholesterol …