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Showing 1 to 20 of 37 for “"Aminoacylation"”.
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Aminoacylation kinetics and specificity for viral genomic RNAs
… (average MW = 1 x 10⁶ ). The rate of TYMV-RNA aminoacylation was extensively studied. In the presence of enzymes from E. coli, yeast and bean, the rate of aminoacylation was inhibited 4 fold by 47.5 mM KCl. In the presence of rat enzyme a 1.5 fold increase in rate was observed. In identical …
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Investigating the Role of Conformational Flexibility in tRNA Aminoacylation
Proteins are dynamic macromolecules. According to Koshland's classical "induced fit" model of enzyme regulation, proteins have essential conformational flexibility for ligand binding, which promotes catalysis by structural rearrangement. Proteins undergo structural rearrangements to bind ligands, …
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Assembly And Function Of Macromolecular Complexes For Accurate Trna Aminoacylation In Helicobacter Pylori
… OF MACROMOLECULAR COMPLEXES FOR ACCURATE TRNA AMINOACYLATION IN <i>HELICOBACTER PYLORI</i> </strong> </p> <p>by</p> <p> <strong>GAYATHRI SILVA</strong> </p> <p>January 2014</p> <p>Advisor: Dr. Tamara L. Hendrickson</p> <p>Major: Chemistry (Biochemistry)</p> <p>Degree: Doctor of Philosophy</p> …
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Biochemical Analysis of the Molecular Factors of Leucyl -Trna Synthetase That Ensure Aminoacylation Fidelity
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.
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Biochemical Analysis of the Molecular Factors of Leucyl-Trna Synthetase That Ensure Aminoacylation Fidelity
The tRNA synthetases catalyze aminoacylation of transfer RNA (tRNA) with specific amino acids. Accurate aminoacylation is critical to the fidelity of protein synthesis. Leucyl-tRNA synthetase (LeuRS) can misactivate a broad scope of non-leucine amino acids. In these cases, mischarged tRNA products …
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2', 3' isomeric specificity at the C-C-A end of tRNA during aminoacylation and protein synthesis.
Thesis. 1975. Ph.D.--Massachusetts Institute of Technology. Dept. of Biology.
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Characterization of Leucyl -Trna Synthetase From Homo Sapiens and Escherichia Coli in Aminoacylation, Amino Acid Editing and Interdomain Interactions
… hscLeuRS possesses a secondary function beyond aminoacylation reliant on a doubly charged Leu-Leu-tRNALeu. Further biochemical analysis of the hscLeuRS focused on its editing pocket. The editing site of hscLeuRS includes a highly conserved threonine discriminator and universally conserved …
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Characterization of Leucyl -Trna Synthetase From Saccharomyces Cerevisiae and Escherichia Coli in Aminoacylation, Amino Acid Editing and Enzyme Inhibition
Because they are essential to protein synthesis, aaRSs offer a promising target for the development of novel drugs. Leucyl-adenylate analogs were tested for inhibition activity against LeuRS from different sources. These analogs efficiently inhibited the E. coli and Saccharomyces cerevisiae …
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Characterization of leucyl-TRNA synthetase from homo sapiens and escherichia coli in aminoacylation, amino acid editing and interdomain interactions
… the translocation of mischarged tRNA from the aminoacylation to the editing active site where mischarged tRNA binds for hydrolysis of the noncognate amino acid to enhance fidelity. Based on crystal structure analysis, the CP1 domain rotates 30° relative to the canonical core where …
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Characterization of Leucyl -Trna Synthetase From Escherichia Coli and Saccharomyces Cerevisiae in Aminoacylation, Amino Acid Editing, and Protein-Dependent RNA Splicing
… domain has uniquely adapted to accommodate aminoacylation, amino acid editing, and RNA-protein interactions.
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tRNA-dependent amino acid discrimination by Escherichia coli valyl-tRNA synthetase
… and cysteine. We also observe low levels of aminoacylation of wild type and 3'-end mutants of tRNAVal with isoleucine. ValRS is unable to hydrolytically deacylate misacylated tRNAVal terminating in 3' pyrimidines, but can deacylate tRNAVal terminating in purines (G or A). Evidently, a purine …
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Potential prebiotic roles of (amino-)acylation in the synthesis and function of RNA
… advantage for their non-enzymatic replication. Aminoacylation of RNA is an important process in modern biology but the intermediacy of aminoacyl-adenylates is considered to be prebiotically implausible. A potentially prebiotic aminoacylation of nucleoside-3'-phosphates, selective for the …
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Nucleotides in yeast phenylanyl - transfer-RNA required for the specific recognition by its cognate yeast phenylalanyl - transfer-RNA synthetase
… Phe}$ mutants were synthesized and their aminoacylation kinetics with FRS determined. In every case where the substitutions of the tertiary nucelotides resulted in a mutant which should maintain the tertiary structure of the tRNA, little effect on the aminoacylation kinetics was observed. …
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Structural And Functional Characterization Of Proteins Of Unknown Function (hp0495, Hp0100 And Hp1259) In Helicobacter Pylori
… Consequently, H. pylori uses an indirect aminoacylation pathway to generate Gln-tRNAGln and Asn-tRNAAsn. Within this process, Asn-tRNAAsn is produced by misacylation of tRNAAsn with aspartate by a non-discriminating aspartyl-tRNA synthetase (ND-AspRS). Next, the heterotrimeric, …
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Characterization of Molecular Structure-Function Relationships of Escherichia Coli Leucyl-Trna Synthetase
… aaRSs have the potential to make mistakes during aminoacylation due to the nature of their amino acid substrate. However, the synthetases have idiosyncratically evolved to include various modules to optimize activity and also enhance fidelity. LeuRS, along with a number of class Ia aaRSs, have …
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Substitution of the Anticodon Loop of Yeast Tyrosine Transfer Ribonucleic Acid (Rna)
… and Pset 1 polynucleotide kinase. The rate of aminoacylation of anticodon loop substituted tRNA('TYR)s by yeast tyrosyl-tRNA synthetase was found to depend upon the sequence of the oligonucleotide inserted. This suggests that the nucleotides in the anticodon loop of yeast tRNA('Tyr) are …
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On the fidelity of translation
… is not strong enough to fully suppress misaminoacylation and mistranslation in these organisms. Pre-transfer editing is considered as a more ancient proofreading pathway, and we investigated its detailed mechanism by unbiased molecular dynamics simulations and umbrella sampling in ∆CP1 …
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Experimental and Computational Dynamics of an Aminoacyl-tRNA Synthetase System
… is dependent upon correct recognition and aminoacylation of transfer RNA (tRNA) by their cognate aminoacyl-tRNA synthetases (AARSs). Methionine is the amino acid that acts as the start codon for every protein and can also be found within a protein message. Methionyl-tRNA synthetase (MetRS) …
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Characterization of the Operational Trna Code for Amino Acids Through Supervised Machine Learning
… or structure-dependent rules governing the aminoacylation of tRNAs outside and independent of the anticodon. Previous efforts to uncover these rules have included experimental studies using synthetic and chemically modified transfer RNAs (tRNAs), as well as computational analyses aimed at …
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Identification and Evolution of New Orthogonal Aminoacyl-tRNA Synthetase/tRNA Pairs for Genetic Code Expansion
… and amino acids) that are orthogonal in their aminoacylation specificity. An orthogonal pair is composed of an aaRS which can interact with its partner tRNA, but not with any other tRNAs in the host, and a tRNA which is substrate to its partner aaRS, but not to any other aaRS in the host. In …
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